ISCte7
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP006704.1 | ND | Comamonas testosteroni | Comamonas testosteroni KF-1 Comamonas testosteroni TK102 |
DNA section
IS Length : 885 bp
Ends
IR Length : 14/16
IRL : GAGCCGCTAACGCCACTTTCCTTTGAGATCAGCCAGAAGCATTTATGCTT
IRR : GAGCGGCAAACACCACCGATCCACGAAGCGCGCACAGATGATCGCAGCGG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCAGCTCGT | TAA | GGATGGCCCC | 0 |
DNA sequence
GAGCCGCTAACGCCACTTTCCTTTGAGATCAGCCAGAAGCATTTATGCTTTTGGCCCTCATGGCAAGACAACCCGTTAGCAAAGAACTGTGGCGACAGCT
GCAGCCCCTGATCCTCGCCTTCACACCTTCCGTCAAAGGTGGCGCACGCAAGCTCACCGTGAGCGATGAAGCAGCGCTCAATGGCATTCTGTTCGTGCAG
CAAACAGGCATCCCATGGGAAGACTTGCCCCAGTCCTTGAATTACGGCAGTGGTATGACCTGCTGGCGGCGCTTGCGTGACTGGAATGCCAATGGCGTTT
GGCCGCGTTTGGTAGCGTTTGCACCAAGCCATGCTGGTACGTCTGCGCGAGCATGACCAGATTGATTGGAGCCGAGCCAGCATAGATGGCTCCTCGGTGC
CAAGCCCCCGGGGGGGCCAGGAAACGGGCCCCAACCCCACCGACAGAGGCAAGCTCGGCTCCAAACGACATATCGTCGTAGATGCCAGAGGCATCCCACT
GTTGATCCTCGTCAGTGGAGCCAACAGGCATGATTCCATGATGTTCGAGAAGTGCATGGATGCGATTGCCGCCATTGCGGGCTTGCCGGGGCGGGCACGC
AAGCGGCCGGCGAAGCTGCATGCGGACAAAGGCTACGATTACAAGCGCTGCCGCGCCTATCTCAGGCGACGAGGCATTACCAGCCGGATTGCCAGGCGAG
GTGTCGAGAGCAGCGAAAAGCTAGGCAAGCATCGCTGGGTTGTAGAGCGCACACATGGCTGGTTTGCAGGTTTTGGCAAGCTGCGCATCCGCTTTGAAAG
GCGGCTGGATATCCACGAAGCGTTGCTGAAACTGGCCGCTGCGATCATCTGTGCGCGCTTCGTGGATCGGTGGTGTTTGCCGCTC
GCAGCCCCTGATCCTCGCCTTCACACCTTCCGTCAAAGGTGGCGCACGCAAGCTCACCGTGAGCGATGAAGCAGCGCTCAATGGCATTCTGTTCGTGCAG
CAAACAGGCATCCCATGGGAAGACTTGCCCCAGTCCTTGAATTACGGCAGTGGTATGACCTGCTGGCGGCGCTTGCGTGACTGGAATGCCAATGGCGTTT
GGCCGCGTTTGGTAGCGTTTGCACCAAGCCATGCTGGTACGTCTGCGCGAGCATGACCAGATTGATTGGAGCCGAGCCAGCATAGATGGCTCCTCGGTGC
CAAGCCCCCGGGGGGGCCAGGAAACGGGCCCCAACCCCACCGACAGAGGCAAGCTCGGCTCCAAACGACATATCGTCGTAGATGCCAGAGGCATCCCACT
GTTGATCCTCGTCAGTGGAGCCAACAGGCATGATTCCATGATGTTCGAGAAGTGCATGGATGCGATTGCCGCCATTGCGGGCTTGCCGGGGCGGGCACGC
AAGCGGCCGGCGAAGCTGCATGCGGACAAAGGCTACGATTACAAGCGCTGCCGCGCCTATCTCAGGCGACGAGGCATTACCAGCCGGATTGCCAGGCGAG
GTGTCGAGAGCAGCGAAAAGCTAGGCAAGCATCGCTGGGTTGTAGAGCGCACACATGGCTGGTTTGCAGGTTTTGGCAAGCTGCGCATCCGCTTTGAAAG
GCGGCTGGATATCCACGAAGCGTTGCTGAAACTGGCCGCTGCGATCATCTGTGCGCGCTTCGTGGATCGGTGGTGTTTGCCGCTC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 297 bp | 103 aa | 60 | 356 | + | No |
Description : First part of the transposase
ORF sequence :
MLLALMARQPVSKELWRQLQPLILAFTPSVKGGARKLTVSDEAALNGILFVQQTGIPWEDLPQSLNYGSGMTCWRRLRDWNANGVWPRLVAFAPSHAGTS
ARA
ARA
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 570 bp | 190 aa | 316 | 885 | + | No |
Description : Second part of the transposase
ORF sequence :
RLHQAMLVRLREHDQIDWSRASIDGSSVPSPRGGQETGPNPTDRGKLGSKRHIVVDARGIPLLILVSGANRHDSMMFEKCMDAIAAIAGLPGRARKRPAK
LHADKGYDYKRCRAYLRRRGITSRIARRGVESSEKLGKHRWVVERTHGWFAGFGKLRIRFERRLDIHEALLKLAAAIICARFVDRWCLPL
LHADKGYDYKRCRAYLRRRGITSRIARRGVESSEKLGKHRWVVERTHGWFAGFGKLRIRFERRLDIHEALLKLAAAIICARFVDRWCLPL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 826 bp | 280 aa | 60 | 885 | + | Yes |
Chemistry : DDE
ORF sequence :
MLLALMARQPVSKELWRQLQPLILAFTPSVKGGARKLTVSDEAALNGILFVQQTGIPWEDLPQSLNYGSGMTCWRRLRDWNANGVWPRVARLHQAMLVRL
REHDQIDWSRASIDGSSVPSPRGGQETGPNPTDRGKLGSKRHIVVDARGIPLLILVSGANRHDSMMFEKCMDAIAAIAGLPGRARKRPAKLHADKGYDYK
RCRAYLRRRGITSRIARRGVESSEKLGKHRWVVERTHGWFAGFGKLRIRFERRLDIHEALLKLAAAIICARFVDRWCLPL
REHDQIDWSRASIDGSSVPSPRGGQETGPNPTDRGKLGSKRHIVVDARGIPLLILVSGANRHDSMMFEKCMDAIAAIAGLPGRARKRPAKLHADKGYDYK
RCRAYLRRRGITSRIARRGVESSEKLGKHRWVVERTHGWFAGFGKLRIRFERRLDIHEALLKLAAAIICARFVDRWCLPL
Blast result :
Comments
ISCte7 is 82% aa similar to ISIS1421.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The STOP codon is done by the insertion site (TAA).
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The STOP codon is done by the insertion site (TAA).
References
1] ISfinder annotation (2018)
2] Schleheck,D., Knepper,T.P., Fischer,K. and Cook,A.M. (2004) Appl. Environ. Microbiol. 70 (7), 4053-4063.
2] Schleheck,D., Knepper,T.P., Fischer,K. and Cook,A.M. (2004) Appl. Environ. Microbiol. 70 (7), 4053-4063.