ISfinder Mirror Site

ISHsal5

Family IS200/IS605
Group IS1341

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP038631	ND	Halobacterium salinarum	Halobacterium salinarum DSM 3754 Halobacterium salinarum 91-R6

DNA section

IS Length : 1687 bp

Ends

Left end : ATAATTGTATTTAGGCGCGAAGTTCCCCTCCTCAAGGAGTCGTTCGAGAGAGCAGAGCTCTCTCGTGATCACGAAATCGGGGATTTTCGGGCAACAACGC II struct. : Yes
Right end : GTGGATACACTTGTATCTGCAAAGCACGTCGTAGAAGCAGGAAGCCCCACTAAATGAGCGAACCGTATCAGCGGTGAGCGAGTCGGGTGGGGTAGTTCAC II struct. : Yes

Insertion site

Left flank	LE cleavage site	Right flank	RE cleavage site
GCGACCAGACTC	ATAA	AACGGGGTACGC	TCAC

DNA sequence

TTGTATTTAGGCGCGAAGTTCCCCTCCTCAAGGAGTCGTTCGAGAGAGCAGAGCTCTCTCGTGATCACGAAATCGGGGATTTTCGGGCAACAACGCAGGC
CGTAGGCTGAGTAGCGTTAACAAGAACACGAAGCGTTCTTGAGATGCAGTAGGGCGGGGAAACAGCGCCCCAGAAATCTTCGGTTTCTGGTGTGTGAGCG
ACACGCTTCGCGCCTCGTCTCCTCGACGCCGTCATCGTCTCCTAAACACTCCGCGACGACTGACCCAGGGACCCATGCAATCACAATCCTTATTTGTGTA
GTTTATGTATAATATGTTGTGGCGACTCGCAAGAACATCTCCATCCGTGACGACCAAGAGGAGTGGATTCAGGACAATCACCTGAACCTTTCCTCGTTCG
TCCAAGAGAAACTGGACGAACTCATCGAGGAACGAGAGTCATAGATGCACTACAACTACAAGTATCGACTCGACCCACCGGAAGCTCTCGCGGAGACGCT
TCTGAATCACGTCGATACTTGTAGGCAACTCTACAACCACGTCCTCTATCTGCTCAACGAGGCAGACGACAGTCCAGCCCGCTACGAAGTGCAGGGACGA
CTCCCGGACCTCAAATCGTGGTGGGGCGACCTGCGCGACGTTCACTCGAAAGTATTGCAGATGGTGGTCAAGCGCGTCTACGACAATCTCTCCACGCTGA
AAGCACAGAAGGAGAACGGATGCGCCGTGGGAAACCTCAAGTGGAAACCGCCCCGAGAGTATCGGTCGCTCACCTACAACCAGTTCGGCTTCAAACTCAA
GAATACGAGTGGTCGGCCTGTCCTGTGGTTGAGCAAAATCGGAGAGATACCGATTCACCTCCACCGAGACATTCCCGAGAACGCGACCATCAAACAGGTC
ACGGTCAAGCAAGAACCCACGGGCGACTGGTTCGCCACGTTCGGCATCGACGTGGACGAAGACACTCCCGAGAAACCGGAGAATCCAGAGCGAGTCATCG
GAATTGACGTTGGTATCCTGAAGTACGTACATGATACGGATGGAATCGCTGTCGAGTCACCAGACTTCTCCGACGAGCGCGAACGATTGGAACGTGCCCA
ACGCGAACTTTCGCGGAAGGAACACGGCTCTGCTAATTGGGAGAAACAACGGCAGGTTGTGGCAGAACGCCACGCCGACCTAAAGCGGAAGCGACGAGAC
TTCCTCCACAAGCTCTCGAACTACTACGCCCGCGAGTATGACCTTGTGGCGGTCGAAGACTTGGACGCGAAGGGTTTGGTCGAACTGCCGAGTAACTCAC
GGAATCGAGCAGGTGCGGCGTGGGGAACGTTCCAGCGGATGCTCGAATCCAAGTGCGAACGCGAGGGAACGCACTTCGTCGCGGTTGACCCGAAGGACAC
GACAAAAGAGTGTGCTTCCTGCGGTGTCAAGACGGACAAACCGCTGTGGGTTCGTGAACACTCCTGTCCCGCCTGCGGGTTCGAGGCGGACAGGGACGCG
AACGCAGCGTGGAATATTCTGTCTCGCGGTCTTGAAGACGTAGGAGTGGGTCACTCCGAATCAACGCCTGTGGAGACTGCGCTCCCTGTGGATACACTTG
TATCTGCAAAGCACGTCGTAGAAGCAGGAAGCCCCACTAAATGAGCGAACCGTATCAGCGGTGAGCGAGTCGGGTGGGGTAGTTCAC

Protein section

ORF number : 2

ORF 1

Length		Begin	End	Strand	Fusion ORF
126 bp	41 aa	319	444	+	No

ORF function : Passenger Gene
Annotation : conserved hypothetical proteinDescription :

ORF sequence :

MATRKNISIRDDQEEWIQDNHLNLSSFVQEKLDELIEERES

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1197 bp	398 aa	445	1641	+	No

ORF function : Accessory Gene
AG : TnpB

ORF sequence :

MHYNYKYRLDPPEALAETLLNHVDTCRQLYNHVLYLLNEADDSPARYEVQGRLPDLKSWWGDLRDVHSKVLQMVVKRVYDNLSTLKAQKENGCAVGNLKW
KPPREYRSLTYNQFGFKLKNTSGRPVLWLSKIGEIPIHLHRDIPENATIKQVTVKQEPTGDWFATFGIDVDEDTPEKPENPERVIGIDVGILKYVHDTDG
IAVESPDFSDERERLERAQRELSRKEHGSANWEKQRQVVAERHADLKRKRRDFLHKLSNYYAREYDLVAVEDLDAKGLVELPSNSRNRAGAAWGTFQRML
ESKCEREGTHFVAVDPKDTTKECASCGVKTDKPLWVREHSCPACGFEADRDANAAWNILSRGLEDVGVGHSESTPVETALPVDTLVSAKHVVEAGSPT

Blast result :

Comments

ISHsal5 is 94% (TnpB) aa similar to ISNamo25.
In the genome, this element is interrupted by a copy of ISHsal2 and a copy of ISNpe16.

References

1] Pfeiffer F., Marchfelder A., Habermann BH., Dyall-Smith M. (2018) Direct submission.
2] Pfeiffer F, Marchfelder A, Habermann B, Dyall-Smith ML. (2019) Microbiol Resour Announc. 11;8(28).