ISWco1
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CAGH01000055 | ND | Weissella confusa | Weissella confusa LBAE C39-2 Weissella paramesenteroides strain FDAARGOS_414 |
DNA section
IS Length : 1434 bp
Ends
IR Length : 18/24
IRL : tgaactgaaccccatgagttggagtaaatctaactcatggggttttctta
IRR : Tgaactgaaccccgaaaattggagtgacgtttaagcggctaactggtcgg
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| gaactgaaccccatgagttggagt | actccaattttcggggttcagttc | 0 |
DNA sequence
TGAACTGAACCCCATGAGTTGGAGTAAATCTAACTCATGGGGTTTTCTTATGTTCTCGAAAGAAATTCAAATTGAAGCAGTATCGATGTATCTCAAAGGT
GTTACGCCCGTTGCAATACGGCAAAAATTCGGAATCAAAGCTCATTCAACTCTTGAAATCTGGGTGAACTCAATCAGACAGGATGGTATTTATGGAATCA
AAGATTCCCACAGAAACAAGACAAGTTATCCGTATTCCTTTAAAATCAAGGTAATTAATTGGCGACTGAATCACAACGCGTCGTATCCCATAACGGCTAG
AAAGTTTAGGATAACAAGTCCATCTTTAATTTGGCATTGGGAACGTGCACTAGAGTCTGGTCGTCTAAAACCTGATAAACGAAGGCGGAAACGAACCATG
AATAAGCAAGAACACGACAAGATCAAGAAGCTCGAAGAAGAGAATGAACTACTTCGGATTAAGGTAGCCTACTTGGAAAAACTCGAGGCCTTAGCTCAGG
AAAAGAAGAAATTACAAACCAAGACAAAGCGGTAGTAATCACTGTGCTAAGGCAAGAATTGAAGTGCAAGTTGAAGCTGCTTCTTCAAGTGGCCGAGATG
TCTAGCAGTAGTTATCATGACGCGTGCAAGCGTGCGTACAAGGAGTATCCACGTGAGTTAATTGATGCGATTATATCAATTCGTGATGACAACCCGGACT
ACGGTTACCGTCGTGTCACACAAGAGCTTCGTAATCGTGGCTTCAACACCAACCATAAGGTTGTGCTGAAAATTATGAAGATGAACGGGCTTCTCTGTAC
TGCTTTCAACCGTCTGACACGGAAGTACAATTCTTACGCCGGCACTGTCGGACATATCTCACCGAATCGTGTAAACCGTCGGTTCACAACGGATCGACCA
TATCAGAAGGTTCTCACTGACGTCACTGAAGTCCGTTGGGGATCAGGCACGATGGCTGAACGTGCTTATTTCACCGCCTATATTGATGTTTATAGTGGTG
AGATTCTCACTTGGAATATCGATCTTCATCCAACTGTCGAGTTCGTCACAAAGCCATTAGATGAGCTTCTACAAAATAGGCCAGAATTGAATTATCGAAT
GACAATTCACTCTGACCAAGGTTTCCAATATCAAAATTATGAGTATGTATCACGGCTAAAGACTAACCGTGTGTTCCAAAGCATGAGTCGCAAAGCGACA
TGCCTAGATAACGCAATGGCTGAGAGTATCTTCCACATCTTGAAGGTAGGAACGGTACATAACAACCACTATCAAAGTTATGACGAATTAAAGTCCGCCA
TAACTAACTATGTCTATTACTACAACAATAAGCGGATAAAAACAAAACTGGCCGGAAGAACTCCGGTTCAATACCGAAATCTTTCCGACCAGTTAGCCGC
TTAAACGTCACTCCAATTTTCGGGGTTCAGTTCA
GTTACGCCCGTTGCAATACGGCAAAAATTCGGAATCAAAGCTCATTCAACTCTTGAAATCTGGGTGAACTCAATCAGACAGGATGGTATTTATGGAATCA
AAGATTCCCACAGAAACAAGACAAGTTATCCGTATTCCTTTAAAATCAAGGTAATTAATTGGCGACTGAATCACAACGCGTCGTATCCCATAACGGCTAG
AAAGTTTAGGATAACAAGTCCATCTTTAATTTGGCATTGGGAACGTGCACTAGAGTCTGGTCGTCTAAAACCTGATAAACGAAGGCGGAAACGAACCATG
AATAAGCAAGAACACGACAAGATCAAGAAGCTCGAAGAAGAGAATGAACTACTTCGGATTAAGGTAGCCTACTTGGAAAAACTCGAGGCCTTAGCTCAGG
AAAAGAAGAAATTACAAACCAAGACAAAGCGGTAGTAATCACTGTGCTAAGGCAAGAATTGAAGTGCAAGTTGAAGCTGCTTCTTCAAGTGGCCGAGATG
TCTAGCAGTAGTTATCATGACGCGTGCAAGCGTGCGTACAAGGAGTATCCACGTGAGTTAATTGATGCGATTATATCAATTCGTGATGACAACCCGGACT
ACGGTTACCGTCGTGTCACACAAGAGCTTCGTAATCGTGGCTTCAACACCAACCATAAGGTTGTGCTGAAAATTATGAAGATGAACGGGCTTCTCTGTAC
TGCTTTCAACCGTCTGACACGGAAGTACAATTCTTACGCCGGCACTGTCGGACATATCTCACCGAATCGTGTAAACCGTCGGTTCACAACGGATCGACCA
TATCAGAAGGTTCTCACTGACGTCACTGAAGTCCGTTGGGGATCAGGCACGATGGCTGAACGTGCTTATTTCACCGCCTATATTGATGTTTATAGTGGTG
AGATTCTCACTTGGAATATCGATCTTCATCCAACTGTCGAGTTCGTCACAAAGCCATTAGATGAGCTTCTACAAAATAGGCCAGAATTGAATTATCGAAT
GACAATTCACTCTGACCAAGGTTTCCAATATCAAAATTATGAGTATGTATCACGGCTAAAGACTAACCGTGTGTTCCAAAGCATGAGTCGCAAAGCGACA
TGCCTAGATAACGCAATGGCTGAGAGTATCTTCCACATCTTGAAGGTAGGAACGGTACATAACAACCACTATCAAAGTTATGACGAATTAAAGTCCGCCA
TAACTAACTATGTCTATTACTACAACAATAAGCGGATAAAAACAAAACTGGCCGGAAGAACTCCGGTTCAATACCGAAATCTTTCCGACCAGTTAGCCGC
TTAAACGTCACTCCAATTTTCGGGGTTCAGTTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 486 bp | 161 aa | 50 | 535 | + | No |
Description : First part of the transposase
ORF sequence :
MFSKEIQIEAVSMYLKGVTPVAIRQKFGIKAHSTLEIWVNSIRQDGIYGIKDSHRNKTSYPYSFKIKVINWRLNHNASYPITARKFRITSPSLIWHWERA
LESGRLKPDKRRRKRTMNKQEHDKIKKLEEENELLRIKVAYLEKLEALAQEKKKLQTKTKR
LESGRLKPDKRRRKRTMNKQEHDKIKKLEEENELLRIKVAYLEKLEALAQEKKKLQTKTKR
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 942 bp | 313 aa | 463 | 1404 | + | No |
Description : Second part of the transposase
ORF sequence :
GSLLGKTRGLSSGKEEITNQDKAVVITVLRQELKCKLKLLLQVAEMSSSSYHDACKRAYKEYPRELIDAIISIRDDNPDYGYRRVTQELRNRGFNTNHKV
VLKIMKMNGLLCTAFNRLTRKYNSYAGTVGHISPNRVNRRFTTDRPYQKVLTDVTEVRWGSGTMAERAYFTAYIDVYSGEILTWNIDLHPTVEFVTKPLD
ELLQNRPELNYRMTIHSDQGFQYQNYEYVSRLKTNRVFQSMSRKATCLDNAMAESIFHILKVGTVHNNHYQSYDELKSAITNYVYYYNNKRIKTKLAGRT
PVQYRNLSDQLAA
VLKIMKMNGLLCTAFNRLTRKYNSYAGTVGHISPNRVNRRFTTDRPYQKVLTDVTEVRWGSGTMAERAYFTAYIDVYSGEILTWNIDLHPTVEFVTKPLD
ELLQNRPELNYRMTIHSDQGFQYQNYEYVSRLKTNRVFQSMSRKATCLDNAMAESIFHILKVGTVHNNHYQSYDELKSAITNYVYYYNNKRIKTKLAGRT
PVQYRNLSDQLAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1355 bp | 451 aa | 50 | 1404 | + | Yes |
Description :
ORF sequence :
MFSKEIQIEAVSMYLKGVTPVAIRQKFGIKAHSTLEIWVNSIRQDGIYGIKDSHRNKTSYPYSFKIKVINWRLNHNASYPITARKFRITSPSLIWHWERA
LESGRLKPDKRRRKRTMNKQEHDKIKKLEEENELLRIKVAYLEKLEALAQEKEEITNQDKAVVITVLRQELKCKLKLLLQVAEMSSSSYHDACKRAYKEY
PRELIDAIISIRDDNPDYGYRRVTQELRNRGFNTNHKVVLKIMKMNGLLCTAFNRLTRKYNSYAGTVGHISPNRVNRRFTTDRPYQKVLTDVTEVRWGSG
TMAERAYFTAYIDVYSGEILTWNIDLHPTVEFVTKPLDELLQNRPELNYRMTIHSDQGFQYQNYEYVSRLKTNRVFQSMSRKATCLDNAMAESIFHILKV
GTVHNNHYQSYDELKSAITNYVYYYNNKRIKTKLAGRTPVQYRNLSDQLAA
LESGRLKPDKRRRKRTMNKQEHDKIKKLEEENELLRIKVAYLEKLEALAQEKEEITNQDKAVVITVLRQELKCKLKLLLQVAEMSSSSYHDACKRAYKEY
PRELIDAIISIRDDNPDYGYRRVTQELRNRGFNTNHKVVLKIMKMNGLLCTAFNRLTRKYNSYAGTVGHISPNRVNRRFTTDRPYQKVLTDVTEVRWGSG
TMAERAYFTAYIDVYSGEILTWNIDLHPTVEFVTKPLDELLQNRPELNYRMTIHSDQGFQYQNYEYVSRLKTNRVFQSMSRKATCLDNAMAESIFHILKV
GTVHNNHYQSYDELKSAITNYVYYYNNKRIKTKLAGRTPVQYRNLSDQLAA
Blast result :
Comments
ISWco1 is 60% aa similar to ISLsa2.
References
1] Korcari D. (2018) Direct submission to ISfinder
2] Fortina M.G., Ricci G., Quattrini M., Korcari D., paper in preparation
2] Fortina M.G., Ricci G., Quattrini M., Korcari D., paper in preparation