ISfinder Mirror Site

ISHsal11

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP038631	ND	Halobacterium salinarum	Halobacterium salinarum DSM 3754 Halobacterium salinarum 91-R6

DNA section

IS Length : 1055 bp

Ends

IR Length : 20/26

IRL : TAGTAACCAGTAGAACTTTTTACTCAGAGTGTCTTGTAGCAGTTAGTGAA
IRR : TAGTAAACATTAGAAGTAATTGCTCACATTAGGGACGGAGAGTTGGTCGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGGGTGGGATGC	TA	TATGAACTGAGA	2

DNA sequence

TAGTAACCAGTAGAACTTTTTACTCAGAGTGTCTTGTAGCAGTTAGTGAAGCATCTCGACGATATCTCCGTCGAAGAACTGCAAGACGCCCTCGACAACG
CCGACGGAAAGAAGCCGACACAACGGCTCTTAGCCGCAATTGCGTACAAGAACGGTGTGACGCAGACCGAACTAGCCGAGTGGTACGACGTTCAGCGGCG
AACGATCTACAGCTGGCTCACGCGACTCGACACCGACGAGTCGCTTGAGCACGCCGCTTCTGATGATAAACGAACTGGGAGAAACAGAAAACTCTCAGAA
ACACAGCAAAACGAATTTGAAGCAACTGTTCACGACCCACCTGAAGAAGTTGGCCTCGACGCGCCGGCGTGGACGCCAGCGCTCGCCCAAGAGTATCTCG
AAGATACGTACGACGTGGAGTACTCAATCCCGAGTTGCCGGCGGTTGCTGAAAGAAGCGGGATTGAGCTATCAAAAACCGCGCCGTTCAGCCGCCGAAGC
CGACGAAGACGACCAAGAAGAATTCCACGACGAGCTCAAAAAAAGCGGCGGGAGATGGACGCCACCGTAGTCTGCATCGACCAAACCAAGAAATCCGTCC
AAGTTGAGCCGCGTGCCGCGTGGTTTCCGCGCGGCACGCGGCCCTCGGTCGAGCTTTCAGGTCAACGTGACTGGACGTGTTTGCTGGGCGCGATCACCGA
GAACGGTGATCGCTTTTTCTCCCGGTTCACCGAGTACGTTACCGCCGATCACGCGAAACATTTCATTTTAGCACTATGCAACGAATTTGAAGATAACTTG
ATCGTCGTGCTTGATGGAGCGTCGTATTTCCAGGCGTCGGTCGTCACGGACCTGGCGGCCCGTGACGACCTCGCCTTCGTGACGTTACCATCGTATTCGC
CGGAGCTAAATCCAGTCGAAGAGTGCTGGAGACAGCTTCAAGCGGCGCTGAGCAACCGCTTCTTTGACTCACTCGACGAGCTGACGACCGCGATTGATAC
CGCTCTCGACCAACTCTCCGTCCCTAATGTGAGCAATTACTTCTAATGTTTACTA

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
525 bp	174 aa	46	570	+	Yes

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKHLDDISVEELQDALDNADGKKPTQRLLAAIAYKNGVTQTELAEWYDVQRRTIYSWLTRLDTDESLEHAASDDKRTGRNRKLSETQQNEFEATVHDPPE
EVGLDAPAWTPALAQEYLEDTYDVEYSIPSCRRLLKEAGLSYQKPRRSAAEADEDDQEEFHDELKKSGGRWTPP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
702 bp	233 aa	345	1046	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

RSWPRRAGVDASARPRVSRRYVRRGVLNPELPAVAERSGIELSKTAPFSRRSRRRRPRRIPRRAQKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAITENGDRFFSRFTEYVTADHAKHFILALCNEFEDNLIVVLDGASYFQASVVTDLAARDDLAFVTLPSYSPELNPVEECWRQLQA
ALSNRFFDSLDELTTAIDTALDQLSVPNVSNYF

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1001 bp	333 aa	46	1046	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKHLDDISVEELQDALDNADGKKPTQRLLAAIAYKNGVTQTELAEWYDVQRRTIYSWLTRLDTDESLEHAASDDKRTGRNRKLSETQQNEFEATVHDPPE
EVGLDAPAWTPALAQEYLEDTYDVEYSIPSCRRLLKEAGLSYQKPRRSAAEADEDDQEEFHDELKKKRREMDATVVCIDQTKKSVQVEPRAAWFPRGTRP
SVELSGQRDWTCLLGAITENGDRFFSRFTEYVTADHAKHFILALCNEFEDNLIVVLDGASYFQASVVTDLAARDDLAFVTLPSYSPELNPVEECWRQLQA
ALSNRFFDSLDELTTAIDTALDQLSVPNVSNYF

Blast result :

Comments

ISHsal11 is 95% aa similar to ISHvo16.The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
In the genome, this element is interrupted by a copy of ISHsal7.

References

1] Pfeiffer F., Marchfelder A., Habermann BH., Dyall-Smith M. (2018) Direct submission.
2] Pfeiffer F, Marchfelder A, Habermann B, Dyall-Smith ML. (2019) Microbiol Resour Announc. 11;8(28).