ISVei4
- Family IS630
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008786 | ND | Verminephrobacter eiseniae | Verminephrobacter eiseniae EF01-2 |
DNA section
IS Length : 1516 bp
Ends
IR Length : 27/32
IRL : GTACCGCTGCACAGAGGTTTTGACGGAATGGCAGGGCTGCAGGACAGGCA
IRR : GTACCTCTGCACAGAGGTTATGACGGTTTGCCGCAAGCTGGTACCGGATA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCAGTGGAC | TA | GATGCAACCC | 2 |
DNA sequence
GTACCGCTGCACAGAGGTTTTGACGGAATGGCAGGGCTGCAGGACAGGCAAGCAGTTGTGGTCAATGAGCAGTTCGATACTGCGCGCCATGCCCGCTTGG
CGGCGGATCAGTCCATCGCGTTCGAGATGGAGCACCATCTGATGGATGGACGGTGCGGTGACACCAAAAAAGCGCTGCATATCACGCTCAGCCGGTGCGC
GGGCGTTGATCAGTCCATAGGCCCAGATGAAAGCCAGATACTGGCCTTGCTTGTTGGTGAACCCCGGGCAGCCTGAACGTGGCTCAGACAACGCCATCGA
TTTTTGATCCATTTGTTGATCCATTCTTGACTCCAGCGATCAAAGGAGGCTCCTTGAAGGAAGCCATCCGAATGAATCTACAGTATCGAGTCGAACTTGA
GCCGTCCGAGCAAGAAATGCTTGCCACCTTGCTGGGCGCAGGCAAGCATGCGGCCCGCACGCTCAAGCGAGCACAAATTCTGCTGGCTGCTCACGCTGGC
CGCGATGACGCGAGCATTGCCGCTACCGTGGCGGTGGGTGAATCCACGGTGTACCGTACCAAGCGCCGCTTCGTGGAGCTGGGCCTGGAGGCTGCGCTTG
CCGAGCAGGCTCGGGCTGGAGCGCAGCGCAAGCTCAGCGGCAAAGAGCAGGCCCTGCTGATTGCCACGGCCTGCGCCAATCCGCCAGCCGGGCGTGCGCG
CTGGACGCTGGAATTGCTGGCCGATGAGTTCGTGAAGCTGACCGAGCACGAAGCACTATCGTGCGAGACGGTACGCAGGCGCCTGGCCGAGAATGATCTG
AAGCCTTGGCGCAAGGACATGTGGTGCATCCCCAAGGTTGACGCTGAATACGTGGCGCGCATGGAGGACGTGCTCGACTTGTATGCCCAAGACCCCGACC
CGCAGCAGCCGGTGGTGTGTTTCGATGAAAGCCCGACCCAGCTCATTGGTGAGGTGCGCCAGCCGATCCCGGCCGAGCCGGGCCAGCCCTTGCGCTATGA
CTACGAATACAAGCGCAACGGCACCGCCAATCTGTTTGTCTTCCTCGATGCGCATCGCAGCTGGCGCAGGGTCAAAGTCACCGAGCGGCGCATGGCCCAG
GACTTTGCCCAGTGCATGCGTGATCTGGTGGACATCCACTACCCGCAGGCCGAGCGCATCCGCCTCGTGCTGGACAACCTGTCAACGCACACGGCTGCTG
CGCTGTACCAATCCATGCCACCACAAGAGGCACGCCGCATCCTGCAGCGCATTGAGTTCCACTACACCCCCAAGCACGCCAGTTGGTTGAACATGGTGGA
GATCGAGATCGGTGTGCTGCGGCGCCAGTGTCTGGATCGCCGCATTGATTGCCGTGAGCGGCTCATTGCTGAGATTGAGGCGTGGGAGCATCTGCGAAAT
GCCAGCGGTGCTCGCATCCACTGGATGTTCTCTACGGACAAGGCCAGGCAAAAGCTAGCCAAGGCCTATCCGGTACCAGCTTGCGGCAAACCGTCATAAC
CTCTGTGCAGAGGTAC
CGGCGGATCAGTCCATCGCGTTCGAGATGGAGCACCATCTGATGGATGGACGGTGCGGTGACACCAAAAAAGCGCTGCATATCACGCTCAGCCGGTGCGC
GGGCGTTGATCAGTCCATAGGCCCAGATGAAAGCCAGATACTGGCCTTGCTTGTTGGTGAACCCCGGGCAGCCTGAACGTGGCTCAGACAACGCCATCGA
TTTTTGATCCATTTGTTGATCCATTCTTGACTCCAGCGATCAAAGGAGGCTCCTTGAAGGAAGCCATCCGAATGAATCTACAGTATCGAGTCGAACTTGA
GCCGTCCGAGCAAGAAATGCTTGCCACCTTGCTGGGCGCAGGCAAGCATGCGGCCCGCACGCTCAAGCGAGCACAAATTCTGCTGGCTGCTCACGCTGGC
CGCGATGACGCGAGCATTGCCGCTACCGTGGCGGTGGGTGAATCCACGGTGTACCGTACCAAGCGCCGCTTCGTGGAGCTGGGCCTGGAGGCTGCGCTTG
CCGAGCAGGCTCGGGCTGGAGCGCAGCGCAAGCTCAGCGGCAAAGAGCAGGCCCTGCTGATTGCCACGGCCTGCGCCAATCCGCCAGCCGGGCGTGCGCG
CTGGACGCTGGAATTGCTGGCCGATGAGTTCGTGAAGCTGACCGAGCACGAAGCACTATCGTGCGAGACGGTACGCAGGCGCCTGGCCGAGAATGATCTG
AAGCCTTGGCGCAAGGACATGTGGTGCATCCCCAAGGTTGACGCTGAATACGTGGCGCGCATGGAGGACGTGCTCGACTTGTATGCCCAAGACCCCGACC
CGCAGCAGCCGGTGGTGTGTTTCGATGAAAGCCCGACCCAGCTCATTGGTGAGGTGCGCCAGCCGATCCCGGCCGAGCCGGGCCAGCCCTTGCGCTATGA
CTACGAATACAAGCGCAACGGCACCGCCAATCTGTTTGTCTTCCTCGATGCGCATCGCAGCTGGCGCAGGGTCAAAGTCACCGAGCGGCGCATGGCCCAG
GACTTTGCCCAGTGCATGCGTGATCTGGTGGACATCCACTACCCGCAGGCCGAGCGCATCCGCCTCGTGCTGGACAACCTGTCAACGCACACGGCTGCTG
CGCTGTACCAATCCATGCCACCACAAGAGGCACGCCGCATCCTGCAGCGCATTGAGTTCCACTACACCCCCAAGCACGCCAGTTGGTTGAACATGGTGGA
GATCGAGATCGGTGTGCTGCGGCGCCAGTGTCTGGATCGCCGCATTGATTGCCGTGAGCGGCTCATTGCTGAGATTGAGGCGTGGGAGCATCTGCGAAAT
GCCAGCGGTGCTCGCATCCACTGGATGTTCTCTACGGACAAGGCCAGGCAAAAGCTAGCCAAGGCCTATCCGGTACCAGCTTGCGGCAAACCGTCATAAC
CTCTGTGCAGAGGTAC
Protein section
ORF number : 2
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 328 bp | 108 aa | 327 | 0 | - | No |
Annotation : Description : Transcriptional Regulator factor
ORF sequence :
MDQQMDQKSMALSEPRSGCPGFTNKQGQYLAFIWAYGLINARAPAERDMQRFFGVTAPSIHQMVLHLERDGLIRRQAGMARSIELLIDHNCLPVLQPCHS
VKTSVQRY
VKTSVQRY
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1128 bp | 375 aa | 372 | 1499 | + | No |
Chemistry : DDE
ORF sequence :
MNLQYRVELEPSEQEMLATLLGAGKHAARTLKRAQILLAAHAGRDDASIAATVAVGESTVYRTKRRFVELGLEAALAEQARAGAQRKLSGKEQALLIATA
CANPPAGRARWTLELLADEFVKLTEHEALSCETVRRRLAENDLKPWRKDMWCIPKVDAEYVARMEDVLDLYAQDPDPQQPVVCFDESPTQLIGEVRQPIP
AEPGQPLRYDYEYKRNGTANLFVFLDAHRSWRRVKVTERRMAQDFAQCMRDLVDIHYPQAERIRLVLDNLSTHTAAALYQSMPPQEARRILQRIEFHYTP
KHASWLNMVEIEIGVLRRQCLDRRIDCRERLIAEIEAWEHLRNASGARIHWMFSTDKARQKLAKAYPVPACGKPS
CANPPAGRARWTLELLADEFVKLTEHEALSCETVRRRLAENDLKPWRKDMWCIPKVDAEYVARMEDVLDLYAQDPDPQQPVVCFDESPTQLIGEVRQPIP
AEPGQPLRYDYEYKRNGTANLFVFLDAHRSWRRVKVTERRMAQDFAQCMRDLVDIHYPQAERIRLVLDNLSTHTAAALYQSMPPQEARRILQRIEFHYTP
KHASWLNMVEIEIGVLRRQCLDRRIDCRERLIAEIEAWEHLRNASGARIHWMFSTDKARQKLAKAYPVPACGKPS
Blast result :
Comments
ISVei4 (transposase) is 84% aa similar to ISAzo9.
OrfA is a passenger gene, that is a potential transcriptionnal repressor.
This gene is on the complementary strand and the stop codon is located on the insertion site.
OrfA is a passenger gene, that is a potential transcriptionnal repressor.
This gene is on the complementary strand and the stop codon is located on the insertion site.
References
1] ISfinder annotation (2008)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stahl,D. and Richardson,P. (2006) Direct Submission GenBank.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Dalin,E., Tice,H., Pitluck,S., Chertkov,O., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Stahl,D. and Richardson,P. (2006) Direct Submission GenBank.