ISAs35

  • Family IS66
  • Group ISBst12
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
KY555070 ND Aeromonas salmonicida
Aeromonas salmonicida subsp. salmonicida 01-B526 plasmid pAsa9
DNA section
IS Length : 1533 bp

Ends


IR Length : 23/27

IRL : GTAACCGTTCACCGAGAGTGGGTTGACATGAAAAATGGGACGATCGTCTC
IRR : GTAACCGTTCACCGGCCGGGGGTTGACGCACACAACTCGACAGGTATGGG

Insertion site


Left flankDirect repeatRight flankDR Length
CCTCATTTTAAGGCTGTGAGAACTGGGGGAGATTCAGTAT0

DNA sequence

GTAACCGTTCACCGAGAGTGGGTTGACATGAAAAATGGGACGATCGTCTCTTGATCCTCCTTTGTCTGTTGTTTGATCCCGCCCGTATTGCATGCTTGCT
GGCATGACCAAAAAGCACTCGACACCCGCGGTTAACTCTCTGACCCTCGACCAGGCCCACGATCTCATACATAAGCTGCTGGACCGTATTACCGAGTTGG
AGGACCGCCTCAACCAGCACAGCGGCAACTCTTCCAAGCCCCCCTCCAGTGACGGGCCAGGAAGTGCTCCGCCCACTCGGCAACGTCCAGCTTCCGCTAA
AAGACGTGGCGCTCAGCCCGGCCATAAAGGCCAGCGGCGGGAACGCCATCTACCCGATGCCCGCCTGTCCGTGGTCTCTTACTATCCGCCTGCGGATTGC
CCCTGCTGTGGTGGCAAGGTGTTGGCCCATGGCAAGCCTTATCGGGTGCATCAGGTGTTTGACCTGCCCGAGGTGAGCTACTTCGTCACCGAGCACCAAC
TGTTTCGTGCTACCTGCTCACAGTGCATCAACACGACGACGGTGACGCTGCCCGAAACGGTGAGTGGTACCCAGATGGGGGCGAGCCTCCTCAGTTACAT
CGCCCTGCAAAGCGGGCAGTTTCATCAGAGTGTCAGCCAGATACAGCAACAGTTGAAACAGCACTTTGGCCTGAGCTTCAGTCGCGGGGCCATCAGCGAA
GCACAAGGGCGGGTCAGTGCCATGCTGACGCCCACTCATCAAGCCATCAAACAGCAGGTGCAGTCGGCCTCGCTTATCCATGCCGATAAAACCCGCCATC
AACGAGGTGGCGAGCGACGCTGGATGTGGATGGCGCTGAGCAAGGTCGCTGTCTGCTTCATGACTGCCTACGGGCGTGGTATCGATGCGGCCAAGCGGTT
GCTGGGGGCAGAGCTGGCAGGCGTGTTGGTGACGGACCAATATGTGGCCTACCGGTTTATCGATGCGAGTCAGCGCCAGCTCTGCTGGGCCCATGTGCTG
CGTAATGTGGCTGCCATCGGACAAAGTGGGGAGCAGGTTAATCAGACCATCGGCACCCGGTTGGTGCTGCTGGCCAACAGTGTGTTTCGTGCTCGCCATC
GCTATGAGGATGGCGAGCTTGGCAAGGAGCGGTATCTACGCCGGCTCAGGAGGTATAGGCAGAGCTGGTTAAAGCAGCTGGAGCAAGGAGCCATGTTGTG
CACCAAGCGCTATCGGGGCCGCTGTGAGTGGCTCATCAAGGATGACGAGATGCTGTGGCGGTTTATGATGGATGACGATATTCCGCTAACGAACAATGAG
GCGGAGCGAGCCCTGCGCGGCTATGTGCTGTGGCGCAAAGGGAGCTACGGCGTGTGTTCACATCGCGGGGAGTTGTTTCGCCAGCGAATACTGTCCTTGG
TGGAGACGGCCAAACGACTGGGGCGTTGTCCTCAGGAGTGGCTGCGCGCCGTTGTCCGAGCCTGCATCGAGAAATCGGATTACCCCATACCTGTCGAGTT
GTGTGCGTCAACCCCCGGCCGGTGAACGGTTAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1422 bp473 aa1041525+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKKHSTPAVNSLTLDQAHDLIHKLLDRITELEDRLNQHSGNSSKPPSSDGPGSAPPTRQRPASAKRRGAQPGHKGQRRERHLPDARLSVVSYYPPADCP
CCGGKVLAHGKPYRVHQVFDLPEVSYFVTEHQLFRATCSQCINTTTVTLPETVSGTQMGASLLSYIALQSGQFHQSVSQIQQQLKQHFGLSFSRGAISEA
QGRVSAMLTPTHQAIKQQVQSASLIHADKTRHQRGGERRWMWMALSKVAVCFMTAYGRGIDAAKRLLGAELAGVLVTDQYVAYRFIDASQRQLCWAHVLR
NVAAIGQSGEQVNQTIGTRLVLLANSVFRARHRYEDGELGKERYLRRLRRYRQSWLKQLEQGAMLCTKRYRGRCEWLIKDDEMLWRFMMDDDIPLTNNEA
ERALRGYVLWRKGSYGVCSHRGELFRQRILSLVETAKRLGRCPQEWLRAVVRACIEKSDYPIPVELCASTPGR

 

Blast result :
Comments
ISAs35 is 88% aa similar to ISKpn15.
References
1] Tanaka,K.H., Vincent,A.T., Emond-Rheault,J.-G., Adamczuk,M., Frenette,M. and Charette,S.J. (2017) Direct GenBank submission.