ISAcp5
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP001472 | ND | Acidobacterium capsulatum | Acidobacterium capsulatum ATCC 51196 |
DNA section
IS Length : 1751 bp
Ends
IR Length : 11
IRL : ATGACATGAGAAGCCGAAGCTTGGATTGCGGCGACACGGTGAGATTCTCG
IRR : TCCATGAGAAGCCACTCCGATGTCAACTATTTTTTCTTTTTTGCTGGCAA
Comments : IRs are not at the termini of the IS.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCCCCATTCA | AG | GGGGCGCACG | 2 |
DNA sequence
ATGACATGAGAAGCCGAAGCTTGGATTGCGGCGACACGGTGAGATTCTCGTCGCCAACGGTTACGAATCAAGGACTCGGATAATGGCTTCTTATGTCAGT
GCCGCCGCAGGCGCCGCGTGAGCGGTCGGAGCGAAGCGCAGAGCCTTGACGGGTTCCGACCGGCTCTGCCATAACGCCGCATGAAGCGGGGGCACTTGAA
CGAAGAAGAAAATTGGGCTACTTCCTTATTGCTGAACGCTTATACGCACGTGGACGGAGACAAAACGTAAGCTGCAATTATCGCGCTGTTGCGCACAGGA
GGTTCGGAATGAATAGAGTGATAGGGATTGACCTCGGAGACAAGAAGAGCCATTACTGCGTATTGGGTGAAGGCGGAGTTGTCGTCGAGGAAGGAACTGT
TGTTAGTACGCCAGCCGCATTCAACGAGCACTTCGGGGTGCTGACATCATCTCTGATTGCCATGGAAGTGGGAGTTCATTCCCGCTGGGCCAGTCGTGTG
TTGCGGGAACTCGGTCACGAGGTAGTCGTGGCAAATCCGGTGCGTCTGAGACTGATCCACAAGAGCAGCAAAAAGAGTGATCGAGTAGATGCGTGCGCTC
TGGCGCGTCTGGTGAGGGTCGATCCCGAGTTACTCGGTCCAGTTGAGCATCGCAGCGACGAAGACCAGAGCATGTTGGCGGTTTTGCGGGTACGAGATTT
GCTGGTGCGCACGCGGACGAGACTGATTAACTGTGCACGCGGCTTGGTGAAGCCCACCGGAACCAGATTGCCGAGTTGTTCCCCAGTGAGCTTCGCCAAT
CGTGCAAGGCCGGCGGTACCGTCGGATTTGCTGCTGGCGCTCGATCCGCTTCTTGATCAGATCCAAAGCCTGACGAACTTGATCAAACGCTACGATCGTA
AGATTGCCAGAATCGCGGAGACGAGTTATCCGGCAACCCAGCATTTAGAGCAGATTCGAGGCGTGGGGCCGCTGACCGCGCTCGGTTTTGTACTGACCAT
TGGACGCAGCAGTCGTATGGTTCACAGCCGCAATGCGGGAGCATATTTTGGACTTAGACCTCGGTGCTACCAATCAGGGGAAAGCAATCCGCAATTGGGA
ATCAGCAAAGAGGGGGATGGATTCATGCGGCGAATGCTGGTGCAGTCCGCACAGTACATCCTGGGTCCGTTCGGTCCGGACACGGATCTGCGGCGATGGG
GCGAACGCTTGATCGCTCGAGGCGGCCGGGGAGCAAGAAGTCGTGCGGTTGTGGCCGTTGCCCGCAAACTCGCTGTTCTTCTGCATCACTTGTGGGTCAC
AGGCGAGGTCTATGATCCGCTGTATAACAGCAGGATCAAGCAACGCGCTCTGGAACCAGTCTCTGCATAACGTTGTTTGTAAGGTACGAAATCGTCGAAA
CCGGAAACGAATGACTGCGGGCGCGCCCTGGCATCACAGAGAGGAGCTCTCCTCCTGTAGACCGAAGTCAAGGTAGCAGCATTCGCCAGTATGACAAGGT
GCACCGGACCGCATGGTCCATTTCAGAGTGCGGATGGAAGGCTGGCGGGCGAAATCTACCAACGTAAGGCTACAGCGGCTGCTTTTCTTTTTTGCTGGGT
GATCTGTGTAGTGCGTTGCGCAGAACCCGTCAAGGGTTCGCTTCGCCGCGCAACAAACACGCGCCCCTGACGGAACCTACGCAACGCACGGAGAACTTTC
CTTGCCAGCAAAAAAGAAAAAATAGTTGACATCGGAGTGGCTTCTCATGGA
GCCGCCGCAGGCGCCGCGTGAGCGGTCGGAGCGAAGCGCAGAGCCTTGACGGGTTCCGACCGGCTCTGCCATAACGCCGCATGAAGCGGGGGCACTTGAA
CGAAGAAGAAAATTGGGCTACTTCCTTATTGCTGAACGCTTATACGCACGTGGACGGAGACAAAACGTAAGCTGCAATTATCGCGCTGTTGCGCACAGGA
GGTTCGGAATGAATAGAGTGATAGGGATTGACCTCGGAGACAAGAAGAGCCATTACTGCGTATTGGGTGAAGGCGGAGTTGTCGTCGAGGAAGGAACTGT
TGTTAGTACGCCAGCCGCATTCAACGAGCACTTCGGGGTGCTGACATCATCTCTGATTGCCATGGAAGTGGGAGTTCATTCCCGCTGGGCCAGTCGTGTG
TTGCGGGAACTCGGTCACGAGGTAGTCGTGGCAAATCCGGTGCGTCTGAGACTGATCCACAAGAGCAGCAAAAAGAGTGATCGAGTAGATGCGTGCGCTC
TGGCGCGTCTGGTGAGGGTCGATCCCGAGTTACTCGGTCCAGTTGAGCATCGCAGCGACGAAGACCAGAGCATGTTGGCGGTTTTGCGGGTACGAGATTT
GCTGGTGCGCACGCGGACGAGACTGATTAACTGTGCACGCGGCTTGGTGAAGCCCACCGGAACCAGATTGCCGAGTTGTTCCCCAGTGAGCTTCGCCAAT
CGTGCAAGGCCGGCGGTACCGTCGGATTTGCTGCTGGCGCTCGATCCGCTTCTTGATCAGATCCAAAGCCTGACGAACTTGATCAAACGCTACGATCGTA
AGATTGCCAGAATCGCGGAGACGAGTTATCCGGCAACCCAGCATTTAGAGCAGATTCGAGGCGTGGGGCCGCTGACCGCGCTCGGTTTTGTACTGACCAT
TGGACGCAGCAGTCGTATGGTTCACAGCCGCAATGCGGGAGCATATTTTGGACTTAGACCTCGGTGCTACCAATCAGGGGAAAGCAATCCGCAATTGGGA
ATCAGCAAAGAGGGGGATGGATTCATGCGGCGAATGCTGGTGCAGTCCGCACAGTACATCCTGGGTCCGTTCGGTCCGGACACGGATCTGCGGCGATGGG
GCGAACGCTTGATCGCTCGAGGCGGCCGGGGAGCAAGAAGTCGTGCGGTTGTGGCCGTTGCCCGCAAACTCGCTGTTCTTCTGCATCACTTGTGGGTCAC
AGGCGAGGTCTATGATCCGCTGTATAACAGCAGGATCAAGCAACGCGCTCTGGAACCAGTCTCTGCATAACGTTGTTTGTAAGGTACGAAATCGTCGAAA
CCGGAAACGAATGACTGCGGGCGCGCCCTGGCATCACAGAGAGGAGCTCTCCTCCTGTAGACCGAAGTCAAGGTAGCAGCATTCGCCAGTATGACAAGGT
GCACCGGACCGCATGGTCCATTTCAGAGTGCGGATGGAAGGCTGGCGGGCGAAATCTACCAACGTAAGGCTACAGCGGCTGCTTTTCTTTTTTGCTGGGT
GATCTGTGTAGTGCGTTGCGCAGAACCCGTCAAGGGTTCGCTTCGCCGCGCAACAAACACGCGCCCCTGACGGAACCTACGCAACGCACGGAGAACTTTC
CTTGCCAGCAAAAAAGAAAAAATAGTTGACATCGGAGTGGCTTCTCATGGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1062 bp | 353 aa | 309 | 1370 | + | No |
Chemistry : DEDD
ORF sequence :
MNRVIGIDLGDKKSHYCVLGEGGVVVEEGTVVSTPAAFNEHFGVLTSSLIAMEVGVHSRWASRVLRELGHEVVVANPVRLRLIHKSSKKSDRVDACALAR
LVRVDPELLGPVEHRSDEDQSMLAVLRVRDLLVRTRTRLINCARGLVKPTGTRLPSCSPVSFANRARPAVPSDLLLALDPLLDQIQSLTNLIKRYDRKIA
RIAETSYPATQHLEQIRGVGPLTALGFVLTIGRSSRMVHSRNAGAYFGLRPRCYQSGESNPQLGISKEGDGFMRRMLVQSAQYILGPFGPDTDLRRWGER
LIARGGRGARSRAVVAVARKLAVLLHHLWVTGEVYDPLYNSRIKQRALEPVSA
LVRVDPELLGPVEHRSDEDQSMLAVLRVRDLLVRTRTRLINCARGLVKPTGTRLPSCSPVSFANRARPAVPSDLLLALDPLLDQIQSLTNLIKRYDRKIA
RIAETSYPATQHLEQIRGVGPLTALGFVLTIGRSSRMVHSRNAGAYFGLRPRCYQSGESNPQLGISKEGDGFMRRMLVQSAQYILGPFGPDTDLRRWGER
LIARGGRGARSRAVVAVARKLAVLLHHLWVTGEVYDPLYNSRIKQRALEPVSA
Blast result :
Comments
ISAcp5 is 68% aa similar to ISCARN52.
There is an empty site in the genome.There is a conflict with the name of this IS, we have renamed it because it used a name (ISAca5) already attributed to another sequence in Acinetobacter calcoaceticus.
There is an empty site in the genome.There is a conflict with the name of this IS, we have renamed it because it used a name (ISAca5) already attributed to another sequence in Acinetobacter calcoaceticus.
References
1] ISfinder submission (2018)
2] Ward,N.L., Challacombe,J.F., Janssen,P.H., Henrissat,B., Coutinho,P.M., Wu,M., Xie,G., Haft,D.H., Sait,M., Badger,J., Barabote,R.D., Bradley,B., Brettin,T.S., Brinkac,L.M., Bruce,D., Creasy,T., Daugherty,S.C., Davidsen,T.M., DeBoy,R.T., Detter,J.C., Dodson,R.J., Durkin,A.S., Ganapathy,A., Gwinn-Giglio,M., Han,C.S., Khouri,H., Kiss,H., Kothari,S.P., Madupu,R., Nelson,K.E., Nelson,W.C., Paulsen,I., Penn,K., Ren,Q., Rosovitz,M.J., Selengut,J.D., Shrivastava,S., Sullivan,S.A., Tapia,R., Thompson,L.S., Watkins,K.L., Yang,Q., Yu,C., Zafar,N., Zhou,L. and Kuske,C.R. (2009) Appl. Environ. Microbiol. 75 (7), 2046-2056.
2] Ward,N.L., Challacombe,J.F., Janssen,P.H., Henrissat,B., Coutinho,P.M., Wu,M., Xie,G., Haft,D.H., Sait,M., Badger,J., Barabote,R.D., Bradley,B., Brettin,T.S., Brinkac,L.M., Bruce,D., Creasy,T., Daugherty,S.C., Davidsen,T.M., DeBoy,R.T., Detter,J.C., Dodson,R.J., Durkin,A.S., Ganapathy,A., Gwinn-Giglio,M., Han,C.S., Khouri,H., Kiss,H., Kothari,S.P., Madupu,R., Nelson,K.E., Nelson,W.C., Paulsen,I., Penn,K., Ren,Q., Rosovitz,M.J., Selengut,J.D., Shrivastava,S., Sullivan,S.A., Tapia,R., Thompson,L.S., Watkins,K.L., Yang,Q., Yu,C., Zafar,N., Zhou,L. and Kuske,C.R. (2009) Appl. Environ. Microbiol. 75 (7), 2046-2056.