ISPye47
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei CCUG 32053 |
DNA section
IS Length : 1362 bp
Ends
IR Length : 11
IRL : ATGGGGTGGACGCCCCCCCGACGGCATCTTTGTGCCAAGGTGAGTCGTTG
IRR : TCTGTGGACGCCCCGTTGGATGCAAGAGGTAAAGTGCAACTGGGCTCGTA
Comments : Insertion within another IS (IS66 family) enabled precisely defining ends and DRs.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| gatcgaaacc | TG | caagatcaat | 2 |
| cctgcagacc | TG | caagatgaac | 2 |
DNA sequence
ATGGGGTGGACGCCCCCCCGACGGCATCTTTGTGCCAAGGTGAGTCGTTGAAAATCACCTAAGGGAGGCGCCCGTGAAGGAAGTTATCACAATTGGACTG
GACATCGCGAAGCATGTTTTCCACGCGCATGGAGCTGACGTTGATGGCCGCATGGTCTTCAGCCGGAAGATCCGGCGTGCGAAGCTGCTGGAGTTCTTTG
CCTCGCAGCAGCCATGCGTGGTTGCACTGGAAGCTTGTGGCGGCGCGCACCACTGGGCCCGTCAGATTACGCAGCAGGGCCATGAGGTGCGCCTGATCCC
GCCGATCTACGTGAAGCCCTTTGTGAAGCGGCAGAAGAACGATGCCGCGGACGCCGAGGCGATTTGCGAGGCAGCACAACGGCCTAGTATGCGCTTTGTA
GCGGTAAAGACCGAGCAACAGCAGGCCTCAGCCATGATCTTCCGCACGCGGGATCTACTGGTCCGCCAGCGCACCCAGCTGGTCAACGCTCTGCGAGGTC
ACCTGACTGAGTATGGCTGGGTGGCACCACAAGGCCGCGGGTATGTCACCATGCTCGCCGATCTTCTCGAAGGCGAGATGGGGCAGTCGCTGCCGGAGGC
AGCACTCGCGATGTTCAAGGTTATGTTGACGACGCTTGGTCATCTTGATCAGCAGATCGACGCGTTGGATCACGAGATCAAGAAGCGCGCACGTGAAGAC
GAAGTTGCGCGGCGGCTCATGACGATCCCAGGCATCGGGCCGATTGCGGCGACAGCCATCGCTGCCCTGGCGCCGCCGCCCGAGCACTTCAAGCGCGCGC
GGGATTTTGCGGCTTGGCTGGGGCTTACACCGCGGCAAATGTCGACTGGTGGCAAGCAAAAGCTGGGCTCCATCTCCAAAATGGGCGAGCGGACATTGCG
ACGGCTGCTCATAATCGGCAGCAGCGCCGTCGTGCTGCATGCTAACTGTCGGGGTGCAGCCCCCGGCTCGTGGTTGGGCCGCATGCTCGCGCGCAAGGCA
CGCATGCTGGTCATCGTCGCGCAAGCCAACAAGACAGCTCGGATCGTATGGGCGGTTTTGATGAAGAAAGAGGACTATAGGACTCCGGTGGCAGCTGCGG
CATAACGCCAAAACTGCGCCAGAGGCTGTCGGGGACGTAGGAGGTCGAAGGAAAGCAGGGCACAACAGTCGGCGAGACGGGGTCGAACAAACCAGAATCT
TGCAATGTGCCAAGAGCACGCAAAACCTGATCTGGCTTCGATCCGCGAACTCCCATGCAGGCCAGCGGCCATAACGCTGCAATCACAGGCCGGACAGATG
ACAGCATCCGACTACGAGCCCAGTTGCACTTTACCTCTTGCATCCAACGGGGCGTCCACAGA
GACATCGCGAAGCATGTTTTCCACGCGCATGGAGCTGACGTTGATGGCCGCATGGTCTTCAGCCGGAAGATCCGGCGTGCGAAGCTGCTGGAGTTCTTTG
CCTCGCAGCAGCCATGCGTGGTTGCACTGGAAGCTTGTGGCGGCGCGCACCACTGGGCCCGTCAGATTACGCAGCAGGGCCATGAGGTGCGCCTGATCCC
GCCGATCTACGTGAAGCCCTTTGTGAAGCGGCAGAAGAACGATGCCGCGGACGCCGAGGCGATTTGCGAGGCAGCACAACGGCCTAGTATGCGCTTTGTA
GCGGTAAAGACCGAGCAACAGCAGGCCTCAGCCATGATCTTCCGCACGCGGGATCTACTGGTCCGCCAGCGCACCCAGCTGGTCAACGCTCTGCGAGGTC
ACCTGACTGAGTATGGCTGGGTGGCACCACAAGGCCGCGGGTATGTCACCATGCTCGCCGATCTTCTCGAAGGCGAGATGGGGCAGTCGCTGCCGGAGGC
AGCACTCGCGATGTTCAAGGTTATGTTGACGACGCTTGGTCATCTTGATCAGCAGATCGACGCGTTGGATCACGAGATCAAGAAGCGCGCACGTGAAGAC
GAAGTTGCGCGGCGGCTCATGACGATCCCAGGCATCGGGCCGATTGCGGCGACAGCCATCGCTGCCCTGGCGCCGCCGCCCGAGCACTTCAAGCGCGCGC
GGGATTTTGCGGCTTGGCTGGGGCTTACACCGCGGCAAATGTCGACTGGTGGCAAGCAAAAGCTGGGCTCCATCTCCAAAATGGGCGAGCGGACATTGCG
ACGGCTGCTCATAATCGGCAGCAGCGCCGTCGTGCTGCATGCTAACTGTCGGGGTGCAGCCCCCGGCTCGTGGTTGGGCCGCATGCTCGCGCGCAAGGCA
CGCATGCTGGTCATCGTCGCGCAAGCCAACAAGACAGCTCGGATCGTATGGGCGGTTTTGATGAAGAAAGAGGACTATAGGACTCCGGTGGCAGCTGCGG
CATAACGCCAAAACTGCGCCAGAGGCTGTCGGGGACGTAGGAGGTCGAAGGAAAGCAGGGCACAACAGTCGGCGAGACGGGGTCGAACAAACCAGAATCT
TGCAATGTGCCAAGAGCACGCAAAACCTGATCTGGCTTCGATCCGCGAACTCCCATGCAGGCCAGCGGCCATAACGCTGCAATCACAGGCCGGACAGATG
ACAGCATCCGACTACGAGCCCAGTTGCACTTTACCTCTTGCATCCAACGGGGCGTCCACAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1032 bp | 343 aa | 74 | 1105 | + | No |
Chemistry : DEDD
ORF sequence :
VKEVITIGLDIAKHVFHAHGADVDGRMVFSRKIRRAKLLEFFASQQPCVVALEACGGAHHWARQITQQGHEVRLIPPIYVKPFVKRQKNDAADAEAICEA
AQRPSMRFVAVKTEQQQASAMIFRTRDLLVRQRTQLVNALRGHLTEYGWVAPQGRGYVTMLADLLEGEMGQSLPEAALAMFKVMLTTLGHLDQQIDALDH
EIKKRAREDEVARRLMTIPGIGPIAATAIAALAPPPEHFKRARDFAAWLGLTPRQMSTGGKQKLGSISKMGERTLRRLLIIGSSAVVLHANCRGAAPGSW
LGRMLARKARMLVIVAQANKTARIVWAVLMKKEDYRTPVAAAA
AQRPSMRFVAVKTEQQQASAMIFRTRDLLVRQRTQLVNALRGHLTEYGWVAPQGRGYVTMLADLLEGEMGQSLPEAALAMFKVMLTTLGHLDQQIDALDH
EIKKRAREDEVARRLMTIPGIGPIAATAIAALAPPPEHFKRARDFAAWLGLTPRQMSTGGKQKLGSISKMGERTLRRLLIIGSSAVVLHANCRGAAPGSW
LGRMLARKARMLVIVAQANKTARIVWAVLMKKEDYRTPVAAAA
Blast result :
Comments
ISPye47 is 77% aa similar to ISPko7.
ISPye47 was identified by in silico sequence analysis of Paracoccus yeei strain CCUG 32053. 2 copies present in the genome, inserted within other IS (IS66 family).
ISPye47 was identified by in silico sequence analysis of Paracoccus yeei strain CCUG 32053. 2 copies present in the genome, inserted within other IS (IS66 family).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.