ISAba46
- Family IS66
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP026864 | ND | Acinetobacter baumannii | Acinetobacter baumannii NCIMB8209 pNCIMB8209_134 |
DNA section
IS Length : 2512 bp
Ends
IR Length : 16
IRL : gtaagcgtccgctgaacacaccttctgaattcatcctataagccttaatt
IRR : gtaagcgtccgctgaatcccataccaatttttTAATTCGATTTAGGTTTC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNNN | gaaagaag | NNNN | 8 |
| CGATTACCAA | TCAGGAAA | ATACCAAATA | 8 |
DNA sequence
GTAAGCGTCCGCTGAACACACCTTCTGAATTCATCCTATAAGCCTTAATTTAGTCCACACTTAAAAACAAGTGGGGACTAAAATTTATGACTACAAATCA
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCGGACGTTTCAATT
GCTTCGGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGCTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCTTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAAAGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGCTTTCTACCC
AACCTCTGGATATGCGAGCAGGGATGGATATTGTCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGCTGGAACAGGGAAAATTCCACTGGGCGCAGGTTCAC
CAGGGTGAAAGCATGGCGATAAGTCCGGAACAGTTACAGGCACTGATCCAGGGTTTACCTTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTTT
AAACCAGACTGTTCCATTCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTCACCAGACAGTTGGCAATGCAGCATCAGTCTCTGGCAGAATCAAATCAAGAGTTAGAAAAATCAAACCAGC
AATTGGATGCCAAAGTTCAACATCTTTCTATTCTCACTCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGCCCAAAAGAATGAACA
CTTAACGGCAAAACAAATCCATCTGTGGGATGAAGCGGTTGAAGAAGATATTGCCGCGGTTGATCTAGAACTGGAGCGGCTGAATGCAGATAAAACCGAT
GCAGTGACACACAAAGCCACAGTCAATAAACCTAAACGTCGACCACTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAAT
GTGCTTGTGGATGCCAACTCCGGCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATATCCGTGGCAA
ATGGGTCTGTGATCAGTGCGACACCCTGACTCAGCAAGCGATGCCAGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTGTTG
GTATCGAAGTATGCCGATCATTTACCGCTGTACCGTCAGCGCCTGATCTATCAGCGGGCGGGCATTGATCTGTCCAGATCCACGTTATCTGACTGGATTG
GTCGCTGCGGTGTGGAACTGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTGCTGCAACAGCGGGTGATCCATGCCGATGAAACGCCGGTCACCATTAT
GCGGATGGGTGAGAATGAGAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACCACACAGTACAATCCAGTTCAAGCGGTGATCTATGACTTTCAG
GATAGCCGTTCAGGTCAGCATGCAGAAGAGTTCCTGAATGGCTGGCAGGGCCATCTGGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GCGATGTCATTGAGGTGGGCTGCATGGCACATGCACGTCGTAAATTTCATGAACTGCATGTGACCGGGAAAAGTCAGATCGCTGAACAGGCATTAGTGCT
GATTCAGAAACTGTATGCCATAGAAGCAGAACTCAGGAAAAAGACCGATAGTACAGCAGAACACCGCCGCGAATACCGACAACAGCACAGCCAACCGGTG
AGGCAACAACTGTATGAATGGCTCAACCAACATGATCTGACGGTGCCATCGAGTTCTCCAACCGCCAAGGCGATCAATTACAGTCTGAAGCGTTGGCCAG
CCTTAAGCCGCTATCTGGATGATGGCAATCTACCCATTGACAATAACTGGGCAGAGAACTCAATGCGTCCCTGGGCATTGGGCCGTAAGAACTGGTTGTT
TGCAGGTTCGCTGCGCAGTGGACAGCGAGCGGCAAATATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGCTTGGATCCGTATGCCTATTTAAGTGAT
GTGCTGAAAAAGCTACCGACACATAAAGTGACCCAGATTGAAGAGTTACTGCCACACTGCTGGAAACCTAAATCGAATTAAAAAATTGGTATGGGATTCA
GCGGACGCTTAC
CCAGACATCCATCGCATCTCTTGCGAAAAAACGAAGAACATACAGTGCTGAATTTAAACAGCAGATCGTTCAGGCTTGTAAAGCACCGGACGTTTCAATT
GCTTCGGTCGCTTTGCAACATGGATTGAATACAAATCTTGTATCCAAATGGATTCGCTTAATTGATGCTAAGCCAGGGAATGATCGCTCACCACTACCGA
ATAAACCTGCATTTATTGCCTTATCTTGCTCTGCACCATTAGATCCTACTCCTACTGACATGTTAAAGGTTCAAATTACTTTACCCCACTCAAAAGCAGA
AATTGGCTTGAAATGGCAAGTATCAGAAATATCTGCTTTAGCAGAATTACTCAAGGCACTTGCAACATGATCCGCATTGATGAAATCTGGCTTTCTACCC
AACCTCTGGATATGCGAGCAGGGATGGATATTGTCATGGCTCAGGTGGTGAGAGCCTTTGGCTACATCAAACCGCATTGTGCTTACCTGTTCTGTAATAA
ACGTGGCCATCGCATGAAAGTGCTGGTACATGATGGACTGGGCATCTGGCTGTGTGCCCGGCGGCTGGAACAGGGAAAATTCCACTGGGCGCAGGTTCAC
CAGGGTGAAAGCATGGCGATAAGTCCGGAACAGTTACAGGCACTGATCCAGGGTTTACCTTGGCAGCGCATTGGACGACAGCAGGTGGTGACGATGCTTT
AAACCAGACTGTTCCATTCTGCTATTCTCCAAACGTTCTATTTCATTCTTCTCATGACCTCAGGCATACTGCGGTCATGAATACGCTGCCTGACTTAAGC
CAACTGACCCATGAACAACTGCTGGAATTCACCAGACAGTTGGCAATGCAGCATCAGTCTCTGGCAGAATCAAATCAAGAGTTAGAAAAATCAAACCAGC
AATTGGATGCCAAAGTTCAACATCTTTCTATTCTCACTCAAAAATACGAGCATGAACTGGCGCTGTTTAAAAAGCACAAATTCGCCCAAAAGAATGAACA
CTTAACGGCAAAACAAATCCATCTGTGGGATGAAGCGGTTGAAGAAGATATTGCCGCGGTTGATCTAGAACTGGAGCGGCTGAATGCAGATAAAACCGAT
GCAGTGACACACAAAGCCACAGTCAATAAACCTAAACGTCGACCACTGCCAGATCATCTACACACCATCCGTATTGAGCATGAACCTGCATCAACCCAAT
GTGCTTGTGGATGCCAACTCCGGCGTATCGGCGAAGATGTCAGTGAAAAACTGCATTTCAGACCGGCACAGTTCTATAAGGAACAGCATATCCGTGGCAA
ATGGGTCTGTGATCAGTGCGACACCCTGACTCAGCAAGCGATGCCAGCCTATGTGATTGATAAAGGCATTGCTTCACCTGAACTGCTCAGCCATGTGTTG
GTATCGAAGTATGCCGATCATTTACCGCTGTACCGTCAGCGCCTGATCTATCAGCGGGCGGGCATTGATCTGTCCAGATCCACGTTATCTGACTGGATTG
GTCGCTGCGGTGTGGAACTGGAACCTCTGGCCAATGCCTTAAAACAGGTGGTGCTGCAACAGCGGGTGATCCATGCCGATGAAACGCCGGTCACCATTAT
GCGGATGGGTGAGAATGAGAAAAAACCGAAGAAAGGTTATGTCTGGGCCTATGCCACCACACAGTACAATCCAGTTCAAGCGGTGATCTATGACTTTCAG
GATAGCCGTTCAGGTCAGCATGCAGAAGAGTTCCTGAATGGCTGGCAGGGCCATCTGGTCTGTGATGATTACAGTGGTTATAAAGCACGCTTTAAATCAG
GCGATGTCATTGAGGTGGGCTGCATGGCACATGCACGTCGTAAATTTCATGAACTGCATGTGACCGGGAAAAGTCAGATCGCTGAACAGGCATTAGTGCT
GATTCAGAAACTGTATGCCATAGAAGCAGAACTCAGGAAAAAGACCGATAGTACAGCAGAACACCGCCGCGAATACCGACAACAGCACAGCCAACCGGTG
AGGCAACAACTGTATGAATGGCTCAACCAACATGATCTGACGGTGCCATCGAGTTCTCCAACCGCCAAGGCGATCAATTACAGTCTGAAGCGTTGGCCAG
CCTTAAGCCGCTATCTGGATGATGGCAATCTACCCATTGACAATAACTGGGCAGAGAACTCAATGCGTCCCTGGGCATTGGGCCGTAAGAACTGGTTGTT
TGCAGGTTCGCTGCGCAGTGGACAGCGAGCGGCAAATATCATGACTTTAATCCAGTCAGCAAAGCTGAATGGCTTGGATCCGTATGCCTATTTAAGTGAT
GTGCTGAAAAAGCTACCGACACATAAAGTGACCCAGATTGAAGAGTTACTGCCACACTGCTGGAAACCTAAATCGAATTAAAAAATTGGTATGGGATTCA
GCGGACGCTTAC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 384 bp | 127 aa | 87 | 470 | + | No |
AG : IS66 TnpA
ORF sequence :
MTTNHQTSIASLAKKRRTYSAEFKQQIVQACKAPDVSIASVALQHGLNTNLVSKWIRLIDAKPGNDRSPLPNKPAFIALSCSAPLDPTPTDMLKVQITLP
HSKAEIGLKWQVSEISALAELLKALAT
HSKAEIGLKWQVSEISALAELLKALAT
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 390 bp | 129 aa | 413 | 802 | + | No |
AG : IS66 TnpB
ORF sequence :
MASIRNICFSRITQGTCNMIRIDEIWLSTQPLDMRAGMDIVMAQVVRAFGYIKPHCAYLFCNKRGHRMKVLVHDGLGIWLCARRLEQGKFHWAQVHQGES
MAISPEQLQALIQGLPWQRIGRQQVVTML
MAISPEQLQALIQGLPWQRIGRQQVVTML
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1605 bp | 534 aa | 877 | 2481 | + | No |
Chemistry : DDE
ORF sequence :
MNTLPDLSQLTHEQLLEFTRQLAMQHQSLAESNQELEKSNQQLDAKVQHLSILTQKYEHELALFKKHKFAQKNEHLTAKQIHLWDEAVEEDIAAVDLELE
RLNADKTDAVTHKATVNKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHIRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIDLSRSTLSDWIGRCGVELEPLANALKQVVLQQRVIHADETPVTIMRMGENEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEEFLNGWQGHLVCDDYSGYKARFKSGDVIEVGCMAHARRKFHELHVTGKSQIAEQALVLIQKLYAIEAELRKKTDSTAEHRREY
RQQHSQPVRQQLYEWLNQHDLTVPSSSPTAKAINYSLKRWPALSRYLDDGNLPIDNNWAENSMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKKLPTHKVTQIEELLPHCWKPKSN
RLNADKTDAVTHKATVNKPKRRPLPDHLHTIRIEHEPASTQCACGCQLRRIGEDVSEKLHFRPAQFYKEQHIRGKWVCDQCDTLTQQAMPAYVIDKGIAS
PELLSHVLVSKYADHLPLYRQRLIYQRAGIDLSRSTLSDWIGRCGVELEPLANALKQVVLQQRVIHADETPVTIMRMGENEKKPKKGYVWAYATTQYNPV
QAVIYDFQDSRSGQHAEEFLNGWQGHLVCDDYSGYKARFKSGDVIEVGCMAHARRKFHELHVTGKSQIAEQALVLIQKLYAIEAELRKKTDSTAEHRREY
RQQHSQPVRQQLYEWLNQHDLTVPSSSPTAKAINYSLKRWPALSRYLDDGNLPIDNNWAENSMRPWALGRKNWLFAGSLRSGQRAANIMTLIQSAKLNGL
DPYAYLSDVLKKLPTHKVTQIEELLPHCWKPKSN
Blast result :
Comments
ISAba46 is 95% aa similar to ISAba16.
References
1] Guillermo Repizo (2018) Direct submission.
2] Repizo GD, Viale AM, Borges V, Cameranesi M, Taib N, Espariz E, Brochier-Armanet C, Gomes JP, Limansky A and Salcedo SP. (2017) Genome Biol Evol. 9:2292-2307.
2] Repizo GD, Viale AM, Borges V, Cameranesi M, Taib N, Espariz E, Brochier-Armanet C, Gomes JP, Limansky A and Salcedo SP. (2017) Genome Biol Evol. 9:2292-2307.