ISAba45
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP026125 | ND | Acinetobacter baumannii | Acinetobacter baumannii NCIMB8209 Acinetobacter baumannii ABNIH28 |
DNA section
IS Length : 1461 bp
Ends
IR Length : 19/26
IRL : TGAACTGCACCCCGAAAGTTGGACACATTCAGTCTAACTTGGAGGGTGCT
IRR : TCTGACCCCATAAAGTTGGACAATTAATTAAGCAGCTTCTAAGGACTGAG
Comments : Empty site in Acinetobacter baumannii strain B8300 (CP021347.1).
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AAAAAACCGCCTA | TCA | TCACAGGCGGTTT | 3 |
DNA sequence
TGAACTGCACCCCGAAAGTTGGACACATTCAGTCTAACTTGGAGGGTGCTTTTCTATGGCTAAATATTCTCAAGAATTTAAACTTGAGGTTGTCCAATAT
TACTTATCTGGTTTTGGCAGACACCAAGTAAGTCATAAATTTGGAATACATCATAGCGATGTTGCTAAATGGGTAGCCAATTATAATAATCATGGTGCTG
AGGGTCTGAGCAGGAAAAACACAAATACTGTATATACTCCTGAATTTAAATTTAAAGTTATTCAGTCTGTTCTTGAACAAGGTTTATCTGTACGTGAAGC
TACAAATCAATTTAATCTGAAATCACACACACAGTTATTAAGTTGGTTGCTCCAATACAAAGAACACGGTATAGATGGCTTAAAAGCTAAGCCGAAAGGA
CGACCTAAATCCGTGCCAAAGCCTAAGATAAAAAAAGTCAAAAATGCTCTGAATGATAGAGACAAAACCCAAGAACAGCTACTAGAAGAACTTGCATATC
TACGAGCTGAGAACGCTTATTTAAAAAAGCGGAGAGCCTTGATTCAGAAGCAGAAAGAACAGGAACAAGCCGAACAGCAACGGTTACAAGATTCATTTCT
GAATTAAGGCAAAACTATAAATTGAAATATCTATTACGTGCATCACAGATGGCTCGAAGCACGTACTTTTATCATGAGCAGCGATCAAAGCTTAACGACA
AATACAGTGACCTTAAGCAGCAAATTAAGATGATCTATCATAAGCATAAAGGTCGGTATGGCTATCGTAGAATTACTCTTGCTTTAAAGAATATGGGATT
AACAATTAACCATAAATGTGTACAAAGACTCATGCAGTCAATGAAACTTAAATCACGCATTAGAATCGCAAAATATCGTTCTTACAAAGGGGACATCGGG
CAAGTTGCAGATAATGTACTACAGCGCCAATTTAAAGCTGACAAACCAAATCAGAAATGGGTAACGGATGTAACCGAGTTTAATATTCGAGGAGATAGGC
TTTACCTATCACCAATTATGGACTTGTTTAATGGGGAAATTATTGCCTTCCAAACCCACCGTAGACCAATGTATGAGTTAGTAAAAAATATGTTGAAAGA
TGCACTTACGAAGCTTGCAGATCATGAAAAGCCTATCATCCATTCAGATCAGGGATGGCAATATCAAATGCGTCATTATCAGCGAGAACTAGCAGAAAAT
GGTTTAACCCAAAGTATGTCTCGTAAAGGAAATTGCTTAGATAATGCATCAATGGAGAGTTTCTTTGGAATACTTAAATCAGAATGTTTCTACGGTGAAA
AATTTAATTCTGTTGAGGAATTAGAGAAAACTGTGAAAGAATACATTCATTATTACAACCATGAAAGAATCAAGGTTAAATTAAAAGGACTAAGCCCTGT
ACAATACAGAACTCAGTCCTTAGAAGCTGCTTAATTAATTGTCCAACTTTATGGGGTCAGA
TACTTATCTGGTTTTGGCAGACACCAAGTAAGTCATAAATTTGGAATACATCATAGCGATGTTGCTAAATGGGTAGCCAATTATAATAATCATGGTGCTG
AGGGTCTGAGCAGGAAAAACACAAATACTGTATATACTCCTGAATTTAAATTTAAAGTTATTCAGTCTGTTCTTGAACAAGGTTTATCTGTACGTGAAGC
TACAAATCAATTTAATCTGAAATCACACACACAGTTATTAAGTTGGTTGCTCCAATACAAAGAACACGGTATAGATGGCTTAAAAGCTAAGCCGAAAGGA
CGACCTAAATCCGTGCCAAAGCCTAAGATAAAAAAAGTCAAAAATGCTCTGAATGATAGAGACAAAACCCAAGAACAGCTACTAGAAGAACTTGCATATC
TACGAGCTGAGAACGCTTATTTAAAAAAGCGGAGAGCCTTGATTCAGAAGCAGAAAGAACAGGAACAAGCCGAACAGCAACGGTTACAAGATTCATTTCT
GAATTAAGGCAAAACTATAAATTGAAATATCTATTACGTGCATCACAGATGGCTCGAAGCACGTACTTTTATCATGAGCAGCGATCAAAGCTTAACGACA
AATACAGTGACCTTAAGCAGCAAATTAAGATGATCTATCATAAGCATAAAGGTCGGTATGGCTATCGTAGAATTACTCTTGCTTTAAAGAATATGGGATT
AACAATTAACCATAAATGTGTACAAAGACTCATGCAGTCAATGAAACTTAAATCACGCATTAGAATCGCAAAATATCGTTCTTACAAAGGGGACATCGGG
CAAGTTGCAGATAATGTACTACAGCGCCAATTTAAAGCTGACAAACCAAATCAGAAATGGGTAACGGATGTAACCGAGTTTAATATTCGAGGAGATAGGC
TTTACCTATCACCAATTATGGACTTGTTTAATGGGGAAATTATTGCCTTCCAAACCCACCGTAGACCAATGTATGAGTTAGTAAAAAATATGTTGAAAGA
TGCACTTACGAAGCTTGCAGATCATGAAAAGCCTATCATCCATTCAGATCAGGGATGGCAATATCAAATGCGTCATTATCAGCGAGAACTAGCAGAAAAT
GGTTTAACCCAAAGTATGTCTCGTAAAGGAAATTGCTTAGATAATGCATCAATGGAGAGTTTCTTTGGAATACTTAAATCAGAATGTTTCTACGGTGAAA
AATTTAATTCTGTTGAGGAATTAGAGAAAACTGTGAAAGAATACATTCATTATTACAACCATGAAAGAATCAAGGTTAAATTAAAAGGACTAAGCCCTGT
ACAATACAGAACTCAGTCCTTAGAAGCTGCTTAATTAATTGTCCAACTTTATGGGGTCAGA
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 552 bp | 183 aa | 56 | 607 | + | No |
Description : First part of the transposase
ORF sequence :
MAKYSQEFKLEVVQYYLSGFGRHQVSHKFGIHHSDVAKWVANYNNHGAEGLSRKNTNTVYTPEFKFKVIQSVLEQGLSVREATNQFNLKSHTQLLSWLLQ
YKEHGIDGLKAKPKGRPKSVPKPKIKKVKNALNDRDKTQEQLLEELAYLRAENAYLKKRRALIQKQKEQEQAEQQRLQDSFLN
YKEHGIDGLKAKPKGRPKSVPKPKIKKVKNALNDRDKTQEQLLEELAYLRAENAYLKKRRALIQKQKEQEQAEQQRLQDSFLN
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 511 | 1434 | + | No |
Description : Second part of the transposase
ORF sequence :
ERLFKKAESLDSEAERTGTSRTATVTRFISELRQNYKLKYLLRASQMARSTYFYHEQRSKLNDKYSDLKQQIKMIYHKHKGRYGYRRITLALKNMGLTIN
HKCVQRLMQSMKLKSRIRIAKYRSYKGDIGQVADNVLQRQFKADKPNQKWVTDVTEFNIRGDRLYLSPIMDLFNGEIIAFQTHRRPMYELVKNMLKDALT
KLADHEKPIIHSDQGWQYQMRHYQRELAENGLTQSMSRKGNCLDNASMESFFGILKSECFYGEKFNSVEELEKTVKEYIHYYNHERIKVKLKGLSPVQYR
TQSLEAA
HKCVQRLMQSMKLKSRIRIAKYRSYKGDIGQVADNVLQRQFKADKPNQKWVTDVTEFNIRGDRLYLSPIMDLFNGEIIAFQTHRRPMYELVKNMLKDALT
KLADHEKPIIHSDQGWQYQMRHYQRELAENGLTQSMSRKGNCLDNASMESFFGILKSECFYGEKFNSVEELEKTVKEYIHYYNHERIKVKLKGLSPVQYR
TQSLEAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1379 bp | 459 aa | 56 | 1434 | + | Yes |
Chemistry : DDE
ORF sequence :
MAKYSQEFKLEVVQYYLSGFGRHQVSHKFGIHHSDVAKWVANYNNHGAEGLSRKNTNTVYTPEFKFKVIQSVLEQGLSVREATNQFNLKSHTQLLSWLLQ
YKEHGIDGLKAKPKGRPKSVPKPKIKKVKNALNDRDKTQEQLLEELAYLRAENAYLKKAESLDSEAERTGTSRTATVTRFISELRQNYKLKYLLRASQMA
RSTYFYHEQRSKLNDKYSDLKQQIKMIYHKHKGRYGYRRITLALKNMGLTINHKCVQRLMQSMKLKSRIRIAKYRSYKGDIGQVADNVLQRQFKADKPNQ
KWVTDVTEFNIRGDRLYLSPIMDLFNGEIIAFQTHRRPMYELVKNMLKDALTKLADHEKPIIHSDQGWQYQMRHYQRELAENGLTQSMSRKGNCLDNASM
ESFFGILKSECFYGEKFNSVEELEKTVKEYIHYYNHERIKVKLKGLSPVQYRTQSLEAA
YKEHGIDGLKAKPKGRPKSVPKPKIKKVKNALNDRDKTQEQLLEELAYLRAENAYLKKAESLDSEAERTGTSRTATVTRFISELRQNYKLKYLLRASQMA
RSTYFYHEQRSKLNDKYSDLKQQIKMIYHKHKGRYGYRRITLALKNMGLTINHKCVQRLMQSMKLKSRIRIAKYRSYKGDIGQVADNVLQRQFKADKPNQ
KWVTDVTEFNIRGDRLYLSPIMDLFNGEIIAFQTHRRPMYELVKNMLKDALTKLADHEKPIIHSDQGWQYQMRHYQRELAENGLTQSMSRKGNCLDNASM
ESFFGILKSECFYGEKFNSVEELEKTVKEYIHYYNHERIKVKLKGLSPVQYRTQSLEAA
Blast result :
Comments
ISAba45 is 71% aa similar to ISBam1.
There are an empty site in Acinetobacter baumannii strain B8300 (CP021347.1).
There are an empty site in Acinetobacter baumannii strain B8300 (CP021347.1).
References
1] Guillermo Repizo (2018) Direct submission.
2] Repizo GD, Viale AM, Borges V, Cameranesi M, Taib N, Espariz E, Brochier-Armanet C, Gomes JP, Limansky A and Salcedo SP. (2017) Genome Biol Evol. 9:2292-2307
2] Repizo GD, Viale AM, Borges V, Cameranesi M, Taib N, Espariz E, Brochier-Armanet C, Gomes JP, Limansky A and Salcedo SP. (2017) Genome Biol Evol. 9:2292-2307