ISfinder Mirror Site

ISAba44

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
	ND	Acinetobacter baumannii	Acinetobacter baumannii NCIMB8209

DNA section

IS Length : 882 bp

Ends

IR Length : 17/26

IRL : GTCAAATCATTATAAAACCAATACAATGGTGCAATGAGTTATTCAGAACA
IRR : GTCAAACTACTTTATTATTCATACAAGATTCGAAAAAAACTCGAAATAAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TTTGGATATA	ta	TAGTAATCAG	0

DNA sequence

GTCAAATCATTATAAAACCAATACAATGGTGCAATGAGTTATTCAGAACATTTTAGACGTAAAATTTTAGCTAAGCTTGAGGAAGGTTATTCTATTCGAG
CAGTAGCTGCACAATTTGAAATTAATAAAAATACCATTGTTGAATGGAAGAAACGTATAGAGATTAAAAAAACTCGTGTACGTAAACCTTCTAAAATTAA
CGATGATGCTTTACGTGAAGATGTTGAAAAGTATCCTGATGCTTATCAATATGAACGTGCTGCACGTTTTAATTGTGGTGTTTCATCTATTGGTGCTGCA
TTAAAGCGCTTAAATATTACAGTTAAAAAAAGACGTTAAGCCATCCTAAAGCAGATACAAAGCTTAGGGATAAATTTATTCTGCAAGTGAATGAATTAGA
AAAAAACCATCCGATTATTTATCTGGATGAAAGTGGTTTTAAGTCACATGATTACAGACCTCATGGCTACTCACGAAAAGGAGTACCCTGTCTAGGGCAA
TATAATTGGCAATTAAAGAATCAGACCAATGCCATTGGAGCAATCCATAAAGGGTATTTATTTTCAGTCGGTTTATTTGACTGCAAAATCAATTCAGATG
TTTTTCATTTTTGGATTGAGCAATTCCTCATACCAGCATTACCAGAAAATAGTGTAGTGGTTATGGATAATGCAGCATTTCATAAAAGAGCAGATATTCA
GGAGTTATTGGAACAACAAGGGCATAAAATTCTTTGGCTTCCTGCATACAGCCCTGATTTAAATCCTATAGAACAGATGTGGGCATGGGTTAAACGTAAG
CGTAAAGAATGGTTGATCGACTCAGTCGATGAGTTATTTCGAGTTTTTTTCGAATCTTGTATGAATAATAAAGTAGTTTGAC

Recoding section

Recoding by frameshift
Frame -1
Type translational
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
306 bp	101 aa	34	339	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSYSEHFRRKILAKLEEGYSIRAVAAQFEINKNTIVEWKKRIEIKKTRVRKPSKINDDALREDVEKYPDAYQYERAARFNCGVSSIGAALKRLNITVKKR
R

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
555 bp	184 aa	327	881	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKTLSHPKADTKLRDKFILQVNELEKNHPIIYLDESGFKSHDYRPHGYSRKGVPCLGQYNWQLKNQTNAIGAIHKGYLFSVGLFDCKINSDVFHFWIEQF
LIPALPENSVVVMDNAAFHKRADIQELLEQQGHKILWLPAYSPDLNPIEQMWAWVKRKRKEWLIDSVDELFRVFFESCMNNKVV

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
848 bp	281 aa	34	881	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSYSEHFRRKILAKLEEGYSIRAVAAQFEINKNTIVEWKKRIEIKKTRVRKPSKINDDALREDVEKYPDAYQYERAARFNCGVSSIGAALKRLNITVKKT
LSHPKADTKLRDKFILQVNELEKNHPIIYLDESGFKSHDYRPHGYSRKGVPCLGQYNWQLKNQTNAIGAIHKGYLFSVGLFDCKINSDVFHFWIEQFLIP
ALPENSVVVMDNAAFHKRADIQELLEQQGHKILWLPAYSPDLNPIEQMWAWVKRKRKEWLIDSVDELFRVFFESCMNNKVV

Blast result :

Comments

ISAba44 is 65% aa similar to ISHde1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] Guillermo Repizo (2018) direct submission.
2] Repizo GD*, Viale AM, Borges V, Cameranesi M, Taib N, Espariz E, Brochier-Armanet C, Gomes JP, Limansky A and Salcedo SP. (2017) Genome Biol Evol. 9:2292-2307.