ISPye39
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei CCUG 32053 |
DNA section
IS Length : 851 bp
Ends
IR Length : 18
IRL : GGCCGCGTTGACAAAGTGGATTCCCAATCTGCCTGATTTCTGATTCACAG
IRR : GGCCGCGTTGACAAAGTGAGGCAGCCATTTGCGGATGGCTGCGAGGTTCA
Comments : Empty insertion site in P. yeei TT13 enabled precisely defining ends.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| ttcggaagac | TA | gcgagaagaa | 2 |
DNA sequence
GGCCGCGTTGACAAAGTGGATTCCCAATCTGCCTGATTTCTGATTCACAGCTTCTCTTTGCGAGGAGCGATGATGGGTCGGGGTGATCTGACAGACGAAG
AATGGGATGTGATCGCGGGACTTCTGCCGTCGGAGCGCGGCCGCAAGTCCCGTCCCGCCCACGATAACCGGCGTTTCCTTAACGGCATGCTTCATGTTCT
GCGGGTCGGCTGTCCGTGGCGGGATATGCACGAACGCTATGGCCTGTGGAACTCGGTCTATGTGCGGTTCCGGCGCTGGGCCGAACAAGGCGTGTGGGAT
GCGATCCTGGCGACATTGGTGGAATTGGGACTGACCGACGACTGGCAACACATGATCGACAGCACCATAGTTCGCGGCCATGTATCGGCGGTGGGCGGAA
AAGGGGGGCTTGTGCGCAGGCGCTTGGTCGATCACGCGGCGGCTTTACGTGCAAGGTCCACGCCCGCTGTGACAATCAGGGACGCCCTATCGGCTTCATC
CTGACGGGCGGTGAAGCGTCCGACTATGGCGCTGTCGATGCCCTGATGGCGATCCCCGTCGCCAAGCCCAAGGCGCTGCTGGCCGACAAGGGCTATGACG
GCGATGTTGTCCGCGAAAATCTGCTGATGCACGGCATCCTGCCGATCATTCCGCCCAAGGCCAATCGCCGCGAACCCATTCCCTGCGACTTCCGCCGCTA
TCGTGACCGCAACCGCATCGAGCGCATGTTCGGGCAACTCAAGCAGTTCCGCCGTATCTCAACCCGTTACGACAAAACCGCCTTATCCTTCGCAAGTTTC
TTGAACCTCGCAGCCATCCGCAAATGGCTGCCTCACTTTGTCAACGCGGCC
AATGGGATGTGATCGCGGGACTTCTGCCGTCGGAGCGCGGCCGCAAGTCCCGTCCCGCCCACGATAACCGGCGTTTCCTTAACGGCATGCTTCATGTTCT
GCGGGTCGGCTGTCCGTGGCGGGATATGCACGAACGCTATGGCCTGTGGAACTCGGTCTATGTGCGGTTCCGGCGCTGGGCCGAACAAGGCGTGTGGGAT
GCGATCCTGGCGACATTGGTGGAATTGGGACTGACCGACGACTGGCAACACATGATCGACAGCACCATAGTTCGCGGCCATGTATCGGCGGTGGGCGGAA
AAGGGGGGCTTGTGCGCAGGCGCTTGGTCGATCACGCGGCGGCTTTACGTGCAAGGTCCACGCCCGCTGTGACAATCAGGGACGCCCTATCGGCTTCATC
CTGACGGGCGGTGAAGCGTCCGACTATGGCGCTGTCGATGCCCTGATGGCGATCCCCGTCGCCAAGCCCAAGGCGCTGCTGGCCGACAAGGGCTATGACG
GCGATGTTGTCCGCGAAAATCTGCTGATGCACGGCATCCTGCCGATCATTCCGCCCAAGGCCAATCGCCGCGAACCCATTCCCTGCGACTTCCGCCGCTA
TCGTGACCGCAACCGCATCGAGCGCATGTTCGGGCAACTCAAGCAGTTCCGCCGTATCTCAACCCGTTACGACAAAACCGCCTTATCCTTCGCAAGTTTC
TTGAACCTCGCAGCCATCCGCAAATGGCTGCCTCACTTTGTCAACGCGGCC
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 73 bp | 143 aa | 72 | 0 | + | No |
Description : First part of the transposase
ORF sequence :
MGRGDLTDEEWDVIAGLLPSERGRKSRPAHDNRRFLNGMLHVLRVGCPWRDMHERYGLWNSVYVRFRRWAEQGVWDAILATLVELGLTDDWQHMIDSTIV
RGHVSAVGGKGGLVRRRLVDHAAALRARSTPAVTIRDALSASS
RGHVSAVGGKGGLVRRRLVDHAAALRARSTPAVTIRDALSASS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 184 bp | 223 aa | 183 | 0 | + | No |
Description : Second part of the transposase
ORF sequence :
RHASCSAGRLSVAGYARTLWPVELGLCAVPALGRTRRVGCDPGDIGGIGTDRRLATHDRQHHSSRPCIGGGRKRGACAQALGRSRGGFTCKVHARCDNQG
RPIGFILTGGEASDYGAVDALMAIPVAKPKALLADKGYDGDVVRENLLMHGILPIIPPKANRREPIPCDFRRYRDRNRIERMFGQLKQFRRISTRYDKTA
LSFASFLNLAAIRKWLPHFVNAA
RPIGFILTGGEASDYGAVDALMAIPVAKPKALLADKGYDGDVVRENLLMHGILPIIPPKANRREPIPCDFRRYRDRNRIERMFGQLKQFRRISTRYDKTA
LSFASFLNLAAIRKWLPHFVNAA
Blast result :Comments : STOP codon within the DR.ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 780 bp | 260 aa | 72 | 851 | + | Yes |
Chemistry : DDE
ORF sequence :
MGRGDLTDEEWDVIAGLLPSERGRKSRPAHDNRRFLNGMLHVLRVGCPWRDMHERYGLWNSVYVRFRRWAEQGVWDAILATLVELGLTDDWQHMIDSTIV
RGHVSAVGGKGGACAQALGRSRGGFTCKVHARCDNQGRPIGFILTGGEASDYGAVDALMAIPVAKPKALLADKGYDGDVVRENLLMHGILPIIPPKANRR
EPIPCDFRRYRDRNRIERMFGQLKQFRRISTRYDKTALSFASFLNLAAIRKWLPHFVNAA
RGHVSAVGGKGGACAQALGRSRGGFTCKVHARCDNQGRPIGFILTGGEASDYGAVDALMAIPVAKPKALLADKGYDGDVVRENLLMHGILPIIPPKANRR
EPIPCDFRRYRDRNRIERMFGQLKQFRRISTRYDKTALSFASFLNLAAIRKWLPHFVNAA
Blast result :Comments : STOP codon within the DR.
Comments
ISPye39 is 60% aa similar to ISPpa6.
ISPye39 was identified by in silico sequence analysis of Paracoccus yeei CCUG32053. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISPye39 was identified by in silico sequence analysis of Paracoccus yeei CCUG32053. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.