ISPye22
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020442 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 |
DNA section
IS Length : 1354 bp
Ends
IR Length : 10
IRL : ATGGTGTGATTGCGGCGGTCGCTCTCCGGTGGTGCACGAAGTCCGGTATG
IRR : TCTCTGATTGCGGCATTGTCAAGCGGGAAAGCGGTTCGTCCGTCCAGGGT
Comments : Insertion within another IS enables precisely defining ends and DR's. The IR of this IS are not at its termini. IRL is separated 6 nt from the left-hand end of the element and 4 nt separate IRR from rigth end.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| cgaacaccat | AC | gggcccgcgg | 2 |
DNA sequence
ATGGTGTGATTGCGGCGGTCGCTCTCCGGTGGTGCACGAAGTCCGGTATGCTGGCCTCACGAGAAAACGCCGTGAGGAGAACGACCATGGACTACTATGC
CGGTTTGGACGTTTCGCTGAAGGAAATCTCGATCTGCGTCGTTGACGAGGGCGGCAGGACCGTTGCCCGAGGTGTCTGTCCTGCTGACCCTGAAGGTGTG
GCGGGCTGGTTTTGCACGAGGTTGCTGTCGCCCAGGAAGATTGTGCATGAAAGCGGGCAGCTATCGATCTGGCTTCAGCGCGGCCTGGAGCGGCTGGGGC
TTCCCGCGCTTTGTATTGACGCCCGCAGGGCGCATAAGAGCCTGTCTGCGCGGCCCAACAAATCCGATGCCGCTGATGCGGAAGGTCTCGCGCAACTGGC
ACGCACAGGCTGGTTCACGCCGGTCCACGTTCGCAGCGAGGAATCAGATCGGCTGCGCTCTCTGATCGGTGCGCGTGCGCAGCTGATCAGCCTCCGCAAG
GACCTCGAAGGCCACGTTCGGGGAATTCTGAAGACCTTCGGCATCCGTTTGACAGGGATCGGGAAAGGTGGGCAGCGGCAGGCCTTTCGTGACCAACTCG
CGGCTGCGGGCGCGCGGGATCTAGTGCTGCGCACTATTGCCGACGGCTTCATCGCCGCCCATGCCACCCTTTGCCAGGCCGCTGCGGAGCTCGATTCTGC
GGTGAAGGAGAAGGCAACGAAGCATCCCGTCGTCCGCCGGTTGATGACCATTCCGGGTGTCGGCGCCATCGTTGCACTGAGCTTTGTCGCTCTGGTCGAT
GATCCGGGGCGGTTTCAGAAATCCGAGAATGTTGGTGCCTTCCTGGGCCTCACTCCTCGAAGGCATCAGTCTGGAGGAGTGGATTGGTCCGGGCGCATAT
CGAAATGCGGGGATGGGGCCATGCGGAACCTGCTGTTCGAGGCGGCGAGTGCCTTGATCGGTCGGGTACAGCGTTTCTCGACGCTGAAAAGCTGGGCTGT
GCGGCTTGCTGGACGGCGCGGGTTCGCCAAGGCGGCTGTGGCGACCGCGCGGAAGCTCGCTGTGCTGATGCTGACGTTGTGGAAAAACGAGACCGAGTAC
AAATGGAAAGAGGAGGCCGTAGCCTGACCTGAGCTCCCGCCCCGGGCGGCGGGCAGAGCGGTCCCGAAGGGGGCGGAGGTTGGTCGATCTCGTGCTGGGC
CGATGGGGATGCGGATCATTCCGCACAACCCGATCCATACATTGGAGACGACCGCCACCGGATACCATCATGAGGCGCAAATCTCGACCTCGAAGAGAAC
CATGACCCTGGACGGACGAACCGCTTTCCCGCTTGACAATGCCGCAATCAGAGA
CGGTTTGGACGTTTCGCTGAAGGAAATCTCGATCTGCGTCGTTGACGAGGGCGGCAGGACCGTTGCCCGAGGTGTCTGTCCTGCTGACCCTGAAGGTGTG
GCGGGCTGGTTTTGCACGAGGTTGCTGTCGCCCAGGAAGATTGTGCATGAAAGCGGGCAGCTATCGATCTGGCTTCAGCGCGGCCTGGAGCGGCTGGGGC
TTCCCGCGCTTTGTATTGACGCCCGCAGGGCGCATAAGAGCCTGTCTGCGCGGCCCAACAAATCCGATGCCGCTGATGCGGAAGGTCTCGCGCAACTGGC
ACGCACAGGCTGGTTCACGCCGGTCCACGTTCGCAGCGAGGAATCAGATCGGCTGCGCTCTCTGATCGGTGCGCGTGCGCAGCTGATCAGCCTCCGCAAG
GACCTCGAAGGCCACGTTCGGGGAATTCTGAAGACCTTCGGCATCCGTTTGACAGGGATCGGGAAAGGTGGGCAGCGGCAGGCCTTTCGTGACCAACTCG
CGGCTGCGGGCGCGCGGGATCTAGTGCTGCGCACTATTGCCGACGGCTTCATCGCCGCCCATGCCACCCTTTGCCAGGCCGCTGCGGAGCTCGATTCTGC
GGTGAAGGAGAAGGCAACGAAGCATCCCGTCGTCCGCCGGTTGATGACCATTCCGGGTGTCGGCGCCATCGTTGCACTGAGCTTTGTCGCTCTGGTCGAT
GATCCGGGGCGGTTTCAGAAATCCGAGAATGTTGGTGCCTTCCTGGGCCTCACTCCTCGAAGGCATCAGTCTGGAGGAGTGGATTGGTCCGGGCGCATAT
CGAAATGCGGGGATGGGGCCATGCGGAACCTGCTGTTCGAGGCGGCGAGTGCCTTGATCGGTCGGGTACAGCGTTTCTCGACGCTGAAAAGCTGGGCTGT
GCGGCTTGCTGGACGGCGCGGGTTCGCCAAGGCGGCTGTGGCGACCGCGCGGAAGCTCGCTGTGCTGATGCTGACGTTGTGGAAAAACGAGACCGAGTAC
AAATGGAAAGAGGAGGCCGTAGCCTGACCTGAGCTCCCGCCCCGGGCGGCGGGCAGAGCGGTCCCGAAGGGGGCGGAGGTTGGTCGATCTCGTGCTGGGC
CGATGGGGATGCGGATCATTCCGCACAACCCGATCCATACATTGGAGACGACCGCCACCGGATACCATCATGAGGCGCAAATCTCGACCTCGAAGAGAAC
CATGACCCTGGACGGACGAACCGCTTTCCCGCTTGACAATGCCGCAATCAGAGA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1041 bp | 346 aa | 87 | 1127 | + | No |
Chemistry : DEDD
ORF sequence :
MDYYAGLDVSLKEISICVVDEGGRTVARGVCPADPEGVAGWFCTRLLSPRKIVHESGQLSIWLQRGLERLGLPALCIDARRAHKSLSARPNKSDAADAEG
LAQLARTGWFTPVHVRSEESDRLRSLIGARAQLISLRKDLEGHVRGILKTFGIRLTGIGKGGQRQAFRDQLAAAGARDLVLRTIADGFIAAHATLCQAAA
ELDSAVKEKATKHPVVRRLMTIPGVGAIVALSFVALVDDPGRFQKSENVGAFLGLTPRRHQSGGVDWSGRISKCGDGAMRNLLFEAASALIGRVQRFSTL
KSWAVRLAGRRGFAKAAVATARKLAVLMLTLWKNETEYKWKEEAVA
LAQLARTGWFTPVHVRSEESDRLRSLIGARAQLISLRKDLEGHVRGILKTFGIRLTGIGKGGQRQAFRDQLAAAGARDLVLRTIADGFIAAHATLCQAAA
ELDSAVKEKATKHPVVRRLMTIPGVGAIVALSFVALVDDPGRFQKSENVGAFLGLTPRRHQSGGVDWSGRISKCGDGAMRNLLFEAASALIGRVQRFSTL
KSWAVRLAGRRGFAKAAVATARKLAVLMLTLWKNETEYKWKEEAVA
Blast result :
Comments
ISPye22 is 63% aa similar to ISMpo4.
ISPye22 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. 2 copies of this IS are present in the genome, both inserted in the same position within another IS (IS110 family).
ISPye22 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252. 2 copies of this IS are present in the genome, both inserted in the same position within another IS (IS110 family).
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.