ISCb1
- Family ISNCY
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AJ250468 | ND | Clostridium beijerinckii | Clostridium beijerinckii NCIMB 8052 |
DNA section
IS Length : 1764 bp
Ends
IR Length : 12/16
IRL : TAGGCGTTTGCGAAACGCCGCAACCCGCAGAAATGCGGGATATTTTTTTA
IRR : TAGATAATTGCGAAACTCATTGATAAAAATGAATAATGATTGATTTGAAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCTTTGCTTT | ATGAATTCTA | ATTCTTTTAA | 10 |
| ATTATAATTT | ACGATTGATAT | ACGATTGATAT | 11 |
DNA sequence
TAGGCGTTTGCGAAACGCCGCAACCCGCAGAAATGCGGGATATTTTTTTAGCAAAATAAAATAACTCCTCACTGATTTATGGTAAAATTGAATTGCCTAA
AAACAAAACTACCATATACCTCAGAAAGGAGTCATATATATGATAACATATAAACAGCTTTCTTTGGCAGATGTTTATTCTGATTGCAAAGAAAAATTTG
AAAATGATAAATTCCAATTTCTTTCTTTACTCGAAAACACTATCGACCTTGATGAGTTAGTTCCTGCATCTTTCATTAACCATTTTTACGCTTCCACAGG
AAGGCCGCGAAAATATCAGCTTTATGCTATGATCAGTGCTTTAATTTTACAACGCATTTTTTCTATTCCTACAGATTCTCTTTTAATTATTTTCCTTAAA
TATTCTCAAGAACTAAGAGACTTCTGCGGCTTTTTAAGAGTTCCTGATTCATCTAAGTTTACTCGTTTCAAGCAGGACTTTCTTTTGGACTTACAATCCA
TGTTCGATAATCTTGTTGATATTACTGAACCGATATGCCAACAGATTGATACGGCTCTTGCTTCCATGACTATTTTTGATACTTCAGGCCTTGAAGCATT
TGTCACTGAAAATAATCCTAAATATGCTAATCGCATTATAAAACAGCTTAAAAATTTTAAGAAAGCATGCAATTTAGATGACTCTTATGATCCTTATAAA
GCTGCCTATGGCTCTATGCCATCACACGCTTCAGCCAACCCTGCCATCCAACAAATGTATATTAATGGGCATTTTTGCTATGCTTTTAAATTCGGAATCA
TTACTAATGGCCTCGGTATCGTAAGAGATATTTCGTTCTATAATAAAGATTTTCTCAATGCTCATCCTGATATTATTGTTGAGAAAAAATCTGATTCACC
TGATGAAGATAAATCTCTTGCAGATTCTAAGGCTCTTATCCCTGTTCTCAAAGATTTTTTTAACAAGCATCCATTAATAAGTCCAAAAACCTTTTTAGGA
GATGCTGCTTTTGACAGTATTCAAATTTATCGTGATCTTCTTCTCGAATTAAAATTTGATAAAGCATATATTCCTTTAAAAACTAGGCTTACACTAGAAA
ATGCTGATTGCCCTTTAAACGAAGACGGCATTCCATGCTGTCCGAATGATCCTTCTATTCCAATGAAACGTGAAGGTAGTAAAAGTCATCTTCGTTCTAG
AATTCCAACAATGAAGTTTGTATGCCCTAAAATGAAATGGTTATATAACAAAGAAACCAAAAAGAGCAAACGTGTAACTCAATGTGAAAATCCATGTACA
AATTCCTTATGTGGACGAATGTTTTATATTTATCCTGAAAAGAATCTGCGCGCATATCCTGGAATAACACGAGGAACCCAAGAATGGAGTAATACTTATA
AAATCAGAGTAGCTGTTGAAAAAAATATAAATCACTTCAAATATAGTTACTGCGTGCGGAGGGTAAGACTCAAAATGAGCAAACACTTCATGCTGATTTA
CTTCTAGCCGGAATAACTCAACTAATAACAGTTATCGTGGCAGATAAAATTCATAAACATAAATATATCCGAAGCTTAAAGCCATTAATTGCATAAAATT
TATTTATAAAAATTGGAATTCACCTTCGCTTTATTTCGTGCGCCCTTTTTTAAGAAGTCAACTATTATATTTGTATAGCAGGAACCATTAAATCCATTAA
TAATGTTTTTGCAACTTCAAATCAATCATTATTCATTTTTATCAATGAGTTTCGCAATTATCTA
AAACAAAACTACCATATACCTCAGAAAGGAGTCATATATATGATAACATATAAACAGCTTTCTTTGGCAGATGTTTATTCTGATTGCAAAGAAAAATTTG
AAAATGATAAATTCCAATTTCTTTCTTTACTCGAAAACACTATCGACCTTGATGAGTTAGTTCCTGCATCTTTCATTAACCATTTTTACGCTTCCACAGG
AAGGCCGCGAAAATATCAGCTTTATGCTATGATCAGTGCTTTAATTTTACAACGCATTTTTTCTATTCCTACAGATTCTCTTTTAATTATTTTCCTTAAA
TATTCTCAAGAACTAAGAGACTTCTGCGGCTTTTTAAGAGTTCCTGATTCATCTAAGTTTACTCGTTTCAAGCAGGACTTTCTTTTGGACTTACAATCCA
TGTTCGATAATCTTGTTGATATTACTGAACCGATATGCCAACAGATTGATACGGCTCTTGCTTCCATGACTATTTTTGATACTTCAGGCCTTGAAGCATT
TGTCACTGAAAATAATCCTAAATATGCTAATCGCATTATAAAACAGCTTAAAAATTTTAAGAAAGCATGCAATTTAGATGACTCTTATGATCCTTATAAA
GCTGCCTATGGCTCTATGCCATCACACGCTTCAGCCAACCCTGCCATCCAACAAATGTATATTAATGGGCATTTTTGCTATGCTTTTAAATTCGGAATCA
TTACTAATGGCCTCGGTATCGTAAGAGATATTTCGTTCTATAATAAAGATTTTCTCAATGCTCATCCTGATATTATTGTTGAGAAAAAATCTGATTCACC
TGATGAAGATAAATCTCTTGCAGATTCTAAGGCTCTTATCCCTGTTCTCAAAGATTTTTTTAACAAGCATCCATTAATAAGTCCAAAAACCTTTTTAGGA
GATGCTGCTTTTGACAGTATTCAAATTTATCGTGATCTTCTTCTCGAATTAAAATTTGATAAAGCATATATTCCTTTAAAAACTAGGCTTACACTAGAAA
ATGCTGATTGCCCTTTAAACGAAGACGGCATTCCATGCTGTCCGAATGATCCTTCTATTCCAATGAAACGTGAAGGTAGTAAAAGTCATCTTCGTTCTAG
AATTCCAACAATGAAGTTTGTATGCCCTAAAATGAAATGGTTATATAACAAAGAAACCAAAAAGAGCAAACGTGTAACTCAATGTGAAAATCCATGTACA
AATTCCTTATGTGGACGAATGTTTTATATTTATCCTGAAAAGAATCTGCGCGCATATCCTGGAATAACACGAGGAACCCAAGAATGGAGTAATACTTATA
AAATCAGAGTAGCTGTTGAAAAAAATATAAATCACTTCAAATATAGTTACTGCGTGCGGAGGGTAAGACTCAAAATGAGCAAACACTTCATGCTGATTTA
CTTCTAGCCGGAATAACTCAACTAATAACAGTTATCGTGGCAGATAAAATTCATAAACATAAATATATCCGAAGCTTAAAGCCATTAATTGCATAAAATT
TATTTATAAAAATTGGAATTCACCTTCGCTTTATTTCGTGCGCCCTTTTTTAAGAAGTCAACTATTATATTTGTATAGCAGGAACCATTAAATCCATTAA
TAATGTTTTTGCAACTTCAAATCAATCATTATTCATTTTTATCAATGAGTTTCGCAATTATCTA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1368 bp | 455 aa | 140 | 1507 | + | No |
Chemistry : Unknow
ORF sequence :
MITYKQLSLADVYSDCKEKFENDKFQFLSLLENTIDLDELVPASFINHFYASTGRPRKYQLYAMISALILQRIFSIPTDSLLIIFLKYSQELRDFCGFLR
VPDSSKFTRFKQDFLLDLQSMFDNLVDITEPICQQIDTALASMTIFDTSGLEAFVTENNPKYANRIIKQLKNFKKACNLDDSYDPYKAAYGSMPSHASAN
PAIQQMYINGHFCYAFKFGIITNGLGIVRDISFYNKDFLNAHPDIIVEKKSDSPDEDKSLADSKALIPVLKDFFNKHPLISPKTFLGDAAFDSIQIYRDL
LLELKFDKAYIPLKTRLTLENADCPLNEDGIPCCPNDPSIPMKREGSKSHLRSRIPTMKFVCPKMKWLYNKETKKSKRVTQCENPCTNSLCGRMFYIYPE
KNLRAYPGITRGTQEWSNTYKIRVAVEKNINHFKYSYCVRRVRLKMSKHFMLIYF
VPDSSKFTRFKQDFLLDLQSMFDNLVDITEPICQQIDTALASMTIFDTSGLEAFVTENNPKYANRIIKQLKNFKKACNLDDSYDPYKAAYGSMPSHASAN
PAIQQMYINGHFCYAFKFGIITNGLGIVRDISFYNKDFLNAHPDIIVEKKSDSPDEDKSLADSKALIPVLKDFFNKHPLISPKTFLGDAAFDSIQIYRDL
LLELKFDKAYIPLKTRLTLENADCPLNEDGIPCCPNDPSIPMKREGSKSHLRSRIPTMKFVCPKMKWLYNKETKKSKRVTQCENPCTNSLCGRMFYIYPE
KNLRAYPGITRGTQEWSNTYKIRVAVEKNINHFKYSYCVRRVRLKMSKHFMLIYF
Blast result :
Comments
This IS disrupted the araC/xyls-like ORF.
References
1] Young,M. (1999) Direct Submission
2] Liyanage,H., Holcroft,P., Evans,V.J., Keis,S., Wilkinson,S.R., Kashket,E.R. and Young,M. (2000) J. Mol. Microbiol. Biotechnol. 2 (1), 107-113
2] Liyanage,H., Holcroft,P., Evans,V.J., Keis,S., Wilkinson,S.R., Kashket,E.R. and Young,M. (2000) J. Mol. Microbiol. Biotechnol. 2 (1), 107-113