ISfinder Mirror Site

ISPye8

Family IS66
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP020442	ND	Paracoccus yeei	Paracoccus yeei Paracoccus yeei FDAARGOS_252

DNA section

IS Length : 2527 bp

Ends

IR Length : 18/22

IRL : GTAAGCGCGACGCTGATCCCCTCCTGCCGCTGGTGATCTGAACGGGCCAA
IRR : GTAAGCGCGACGACGAGGCCCTATGCAGCGAGGCTTGACGCTTGGTCGTA

Comments : Insertion within another IS enabled precisely defining ends and DRs.

Insertion site

Left flank	Direct repeat	Right flank	DR Length
atggttcctc	ACATGAAA	aagcccggag	8
caggacccat	TGATCATC	tgctcgcggc	8
cagcggaatg	AGAGAGGT	cgcctcacaa	8

DNA sequence

GTAAGCGCGACGCTGATCCCCTCCTGCCGCTGGTGATCTGAACGGGCCAATCTCCGTGCGCATGAACGTCGGAGGTGTTGGCTTGGAACGAGACGGCAAA
ATGGGCGGCCATTTGGACGGCTTCTCGGATGGTGGGGTTTCGCGGCTGGAAGTGATGGAGGGGCCGACCGGGCGTCAGCGACGCACGAAGGCGGAGCGGG
CGCGGATTGCGGCGGAAAGCTTGGTGCCGGGAACTTCGGTGACCGAAGTCGCGCGGCGGCACGGGACGACGCGCTGGCAGGTTTATGACTGGCGGAAGAA
GCTACGGGACGGACAGCTTGTCGTGCCCGAGAGCGTGGCGGCACTGCCGATGTTTGCAGAACTGGTCGTCGAGGGCGCCGCAGCGGAGATGCCGGCAGAG
ACGGAGGCCAGCACCGGCGTGGAGATTGTTGTGGGTGATGTCGTGATCCGCGTCGGCGCAGATACCGATGAGGTTCTGCTTACGCGGTCGATCCGAGCGG
CGCGGGCGGCGGCATGGTGATGCTCGGCCAGGGCGGGCCGGTGAAGGTCTACGTGGCGACGCGGCCGGTGGACTTCCGCAAGGGCATCGACGGGCTGGCG
CTGGCGGTGCAGGAGATGTTCGGGCTCGACCCGTTCTGCGGGGCGGTCTTCGTCTTCCGGGCGAAGCGGGCAGATCGTATCAAGATTCTGGTCTGGGACC
AGACCGGGATGGTGCTCGTTCACAAGCGCCTCGAGGGCGGCAAGTTCGTCTGGCCGCAGGTGCGGGATGGGGTGATGCGCCTGTCCTCGGCGCAGCTGGC
GGCGCTGTTCGAGGGGTTGGACTGGAGACTGGTAAGACCCGAGCGGGTGCGCCGCCCGCTGGTTGCAGGGTAAGTGCATCCGACGCACTGGAGGCCCCGT
TTTTACTGGCCAGAACGGCATCAGCGTGATTCACTTCAGGCATGGATGCCGACACTCTCAGCCGCGAGAACGCTCATCTGAAGGCCCGCCTCGCGGAGGT
CGAGGCAGCGCTCGCGGAGGTACAGGAGGCAAACCGCCGGCTGGAGGGTATCCTGCGCACCTCGCAGCGCGAGCGCTTTGGCAAGAGTTCCGAGAAGTTG
TCACCCGACCAGTTCAATTTGCCGCTCGAGGACGCCGAACTGGCCCAGGGCGTGCTTGAGGCCGCGCAGGAGAAGGCTGAAGCCGCGCTTTCCCGGGCGC
GAGGGGAAGAACCTCGCAAGCCCGCGCGCAACCGTGGGCATCTGCCGGCGCATCTGCCCCGGGTCGAGCGCGTGATCGAGCCGGCCAGCACCCTTTGCCC
CTGCGGTTGTGGTGAGATGGCCCGGATCGGGGAGGATGTCTCCGAGCGGCTCGACGTGATCCCGGCGCAGTTCCAGGTTCTTGTGACGCGGCGCCCCAGA
TATGCCTGTCGCCGCTGCTCGCAGGCCGTAGTGCAGGCTCATGCGCCCGAGCACGTCGTGCCGGGCGGCCTGCCCACCGAGCGGCTGATCGCATGGATCA
TCATCTCGAAGTTCGGCGACCACCTTCCGTTTTACCGCCAGGCGGGGATCTTCGAGCGGCAGGGGCTCCACCTCGACCGCGGCACATTCGGCAATTGGGT
CGGCCGCGCCTGCTTCCACCTGATGCCCGTGATCGACCACATGAAGGTCCACCTGCGCGTCGCCGACCGGATCTTCGTCGACGAGACCCGCGCGCCTGTT
CTGGAGCCGGGCCGGAAGGCCACCAAGAGCGGCTACTTCTGGGCCGTCGTATCCGACGATCGCGGGCATGGCGGTGCCGGCCCGCCCCTCGTGCTGTTCC
ACTACGCTCCCGGTCGGGGACGGGAACATCCGCTTAGGTTCCTGGCCGGATACCGCGGCCGGTTCCTGCAATGCGACGCCTACCAATCCTACAATGCGCT
GACCGAGATCACGCGCGATGAGGGGCCGTGGCAGCTGGTCTATTGCTGGACCCACGTCCGCCGCCGCTTCGTCAAACGCGTCGAGAGCGACGGCTCGCCC
ATCGCCGAGGAGATGCTGCGCCAGATCGCATTGCTGTATCAGATCGAAAAGACCGTGCGCGGCAAGGACCCGGCCGTCCGCATGGCCATCCGACGCGAGC
AATCCGCCCCGATCATCTCCGCGCTCAAGCCCTGGCTGGAGACCCAGCTCTCGCGCATCCCGCGGAAGTCCCTGCTGGCCGAGGATATCCGCTACACCCT
CGCGCACTGGCCCGGCCTGATCCGCTTTATCGACGATGGCACCCTCGAGCTGGACACCAACCCGGTCGAAAACCAGATCCGTCCGATCGCGCTGACCCGG
AAGAACGCCCTCTTCGCCGGGAACGAAATCGGCGCCGAGAACTGGGCCATGCTCGCCTCGCTGGTTGCCACCTGCAAGCTGTCCGGCGTGAACCCCGTCG
ACTACCTCGCCGATACCCTCCGGGCCATCCTCGACGGACACCCCAGATCCCGCATCGAGGACCTCATGCCCTGGTGCTACGACCAAGCGTCAAGCCTCGC
TGCATAGGGCCTCGTCGTCGCGCTTAC

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
438 bp	119 aa	83	520	+	No

ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

LERDGKMGGHLDGFSDGGVSRLEVMEGPTGRQRRTKAERARIAAESLVPGTSVTEVARRHGTTRWQVYDWRKKLRDGQLVVPESVAALPMFAELVVEGAA
AEMPAETEASTGVEIVVGDVVIRVGADTDEVLLTRSIRAARAAAW

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
333 bp	119 aa	541	873	+	No

ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MVMLGQGGPVKVYVATRPVDFRKGIDGLALAVQEMFGLDPFCGAVFVFRAKRADRIKILVWDQTGMVLVHKRLEGGKFVWPQVRDGVMRLSSAQLAALFE
GLDWRLVRPERVRRPLVAG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1566 bp	521 aa	942	2507	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDADTLSRENAHLKARLAEVEAALAEVQEANRRLEGILRTSQRERFGKSSEKLSPDQFNLPLEDAELAQGVLEAAQEKAEAALSRARGEEPRKPARNRGH
LPAHLPRVERVIEPASTLCPCGCGEMARIGEDVSERLDVIPAQFQVLVTRRPRYACRRCSQAVVQAHAPEHVVPGGLPTERLIAWIIISKFGDHLPFYRQ
AGIFERQGLHLDRGTFGNWVGRACFHLMPVIDHMKVHLRVADRIFVDETRAPVLEPGRKATKSGYFWAVVSDDRGHGGAGPPLVLFHYAPGRGREHPLRF
LAGYRGRFLQCDAYQSYNALTEITRDEGPWQLVYCWTHVRRRFVKRVESDGSPIAEEMLRQIALLYQIEKTVRGKDPAVRMAIRREQSAPIISALKPWLE
TQLSRIPRKSLLAEDIRYTLAHWPGLIRFIDDGTLELDTNPVENQIRPIALTRKNALFAGNEIGAENWAMLASLVATCKLSGVNPVDYLADTLRAILDGH
PRSRIEDLMPWCYDQASSLAA

Blast result :

Comments

ISPye8 is 68% aa similar to ISRhsp5.
ISPye8 was identified by in silico nucleotide sequence analysis of Paracoccus yeei strain FDAARGOS_252 (3 copies of ISPye8 present in the genome).

References

1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.