ISPye4
- Family IS1182
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP020442 | ND | Paracoccus yeei | Paracoccus yeei Paracoccus yeei FDAARGOS_252 |
DNA section
IS Length : 1509 bp
Ends
IR Length : 17/18
IRL : GGGGTCGTTGAAAAAGCTATCTTTGAATGATTCACTGGCCTCAGATCGTA
IRR : GGGGTCGTTGAAAAAGTTGCTTCTGTGGCGACTGAATACAGATCGGGGCG
Comments : Insertion within another IS enabled precisely defining ends and DR's.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| gatcaggtga | ACGAT | gcaggtctgg | 5 |
| ccagacctgt | ATCGT | ccatctgatc | 5 |
| gatcaggtga | ACGAT | gcaggtctgg | 5 |
DNA sequence
GGGGTCGTTGAAAAAGCTATCTTTGAATGATTCACTGGCCTCAGATCGTAGCGGAGGCGGGCATGCTGGGTCGCAAGGAGCGGGATCAACTGGAGCTGTT
CATTTCAGGATCGCTTCGCCAACTTATTCCTGACGAGCATGTCCTGGTTCGGGTCGATGCTGTTCTCGACCTTACCTGGCTTCGCGACGATATTGCTGAT
CTCTACTGCGCCGACAACGGCCGACCGGGCATTGATCCCGAGGTTGCAGTTCGCTTGATGCTGGCAGGTTTCCTGCTCGGCATTGTCCATGACCGGCGAC
TGATGCGTGAGGCGGCGGTCAATATCGCGATCCGCTGGTTTGTTGGTTACGGGCTGCATGAGGCTCTGCCCGATCATTCGTCGCTGACGCGCATTCGCCA
GCGCTGGGGTGCCGAGCGTTTTCGCCGGATCTTCGAACGCACGGTTCAGGCCTGCGTCACCGCGAAGATCGCGACCGGAGAGATTGTCCATCTCGACGCC
TCCTTGATCCGCGCGCATGTGAGCTGGGATTCGCTTGCCGTCCGCCACGTAGAAGCGGTTGTCGATGCCAACGATGAAACGCTCCGCGAGAGCCGACAGA
CCGGCAGGTTCAAGAAAGTTTGCGTGACCGACCCTGACGCCACGATGGCCACGAACGGCCGCAATCGCCGCCTCGAACCGGCCTACAAGCAGCATACTGC
CGTCGATGACGTGGCAGGTGTCGTTGTCGACGTCGAGGTCACGACCGGCGAGGAAAATGAGGGTATGGTGATCGCCGGACGGCTCGATGCTGCGGCTACG
ACCACCGGCCGGGCGATCAGCATCGCGACGATGGATGCCGGCTATGCCTACGCCAAGGTGTTCCGGGTGCTTGAGGATCGCGAGATCGAAGCCATCGTTC
CCGCCAAGGCTGAACAGGCTCCAGGCAAAGTCATTCCTACGCGCCGGTTCAAGTTCGATGCCCGGCACAATCTTGCTCGCTGTCCGCAAGGCAAGATCCT
GCGGCCCAAAGGCAAGCTCCAGCGCGGTGCTTTCCAGTATTTTCATGCCCGCGCCAAGGACTGTGGCGGCTGCCCGTTGCGGGCTCGCTGTGTCAGCCCT
TCACGTCACGCCCGCGTCGTTGTGTTCAATGTCAACCATCCTTCGCTGCTCCGGGCGCGGCGCAGGCGGATTCGGTGGGGCGACCGCGAAGAACGCCTCT
ATCAGCGCCACCGCTGGCGGGTTGAGGGCGTCCATGGCGAAGCCAAGACTTGGCATGGCCTTGCCCGCGCCGTCCGGCGCGGGCTCGACAATATCCGCAT
CCAGGCTTTCCTGACGGCTGCCGCCATCAATCTCAAACGGCTCGCAGTTGCCTTGATCTTGTTGATTTATGCCGACTTGACGGCATACGCCGCGCCAAAT
CGACCCTGCGGGCGCCTCTCGGCTCTTAGGATCCGAAAAACGCGGGCTTCGGAGGTGAACGCCCCGATCTGTATTCAGTCGCCACAGAAGCAACTTTTTC
AACGACCCC
CATTTCAGGATCGCTTCGCCAACTTATTCCTGACGAGCATGTCCTGGTTCGGGTCGATGCTGTTCTCGACCTTACCTGGCTTCGCGACGATATTGCTGAT
CTCTACTGCGCCGACAACGGCCGACCGGGCATTGATCCCGAGGTTGCAGTTCGCTTGATGCTGGCAGGTTTCCTGCTCGGCATTGTCCATGACCGGCGAC
TGATGCGTGAGGCGGCGGTCAATATCGCGATCCGCTGGTTTGTTGGTTACGGGCTGCATGAGGCTCTGCCCGATCATTCGTCGCTGACGCGCATTCGCCA
GCGCTGGGGTGCCGAGCGTTTTCGCCGGATCTTCGAACGCACGGTTCAGGCCTGCGTCACCGCGAAGATCGCGACCGGAGAGATTGTCCATCTCGACGCC
TCCTTGATCCGCGCGCATGTGAGCTGGGATTCGCTTGCCGTCCGCCACGTAGAAGCGGTTGTCGATGCCAACGATGAAACGCTCCGCGAGAGCCGACAGA
CCGGCAGGTTCAAGAAAGTTTGCGTGACCGACCCTGACGCCACGATGGCCACGAACGGCCGCAATCGCCGCCTCGAACCGGCCTACAAGCAGCATACTGC
CGTCGATGACGTGGCAGGTGTCGTTGTCGACGTCGAGGTCACGACCGGCGAGGAAAATGAGGGTATGGTGATCGCCGGACGGCTCGATGCTGCGGCTACG
ACCACCGGCCGGGCGATCAGCATCGCGACGATGGATGCCGGCTATGCCTACGCCAAGGTGTTCCGGGTGCTTGAGGATCGCGAGATCGAAGCCATCGTTC
CCGCCAAGGCTGAACAGGCTCCAGGCAAAGTCATTCCTACGCGCCGGTTCAAGTTCGATGCCCGGCACAATCTTGCTCGCTGTCCGCAAGGCAAGATCCT
GCGGCCCAAAGGCAAGCTCCAGCGCGGTGCTTTCCAGTATTTTCATGCCCGCGCCAAGGACTGTGGCGGCTGCCCGTTGCGGGCTCGCTGTGTCAGCCCT
TCACGTCACGCCCGCGTCGTTGTGTTCAATGTCAACCATCCTTCGCTGCTCCGGGCGCGGCGCAGGCGGATTCGGTGGGGCGACCGCGAAGAACGCCTCT
ATCAGCGCCACCGCTGGCGGGTTGAGGGCGTCCATGGCGAAGCCAAGACTTGGCATGGCCTTGCCCGCGCCGTCCGGCGCGGGCTCGACAATATCCGCAT
CCAGGCTTTCCTGACGGCTGCCGCCATCAATCTCAAACGGCTCGCAGTTGCCTTGATCTTGTTGATTTATGCCGACTTGACGGCATACGCCGCGCCAAAT
CGACCCTGCGGGCGCCTCTCGGCTCTTAGGATCCGAAAAACGCGGGCTTCGGAGGTGAACGCCCCGATCTGTATTCAGTCGCCACAGAAGCAACTTTTTC
AACGACCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1447 bp | 482 aa | 63 | 1509 | + | No |
Chemistry : DDE
ORF sequence :
MLGRKERDQLELFISGSLRQLIPDEHVLVRVDAVLDLTWLRDDIADLYCADNGRPGIDPEVAVRLMLAGFLLGIVHDRRLMREAAVNIAIRWFVGYGLHE
ALPDHSSLTRIRQRWGAERFRRIFERTVQACVTAKIATGEIVHLDASLIRAHVSWDSLAVRHVEAVVDANDETLRESRQTGRFKKVCVTDPDATMATNGR
NRRLEPAYKQHTAVDDVAGVVVDVEVTTGEENEGMVIAGRLDAAATTTGRAISIATMDAGYAYAKVFRVLEDREIEAIVPAKAEQAPGKVIPTRRFKFDA
RHNLARCPQGKILRPKGKLQRGAFQYFHARAKDCGGCPLRARCVSPSRHARVVVFNVNHPSLLRARRRRIRWGDREERLYQRHRWRVEGVHGEAKTWHGL
ARAVRRGLDNIRIQAFLTAAAINLKRLAVALILLIYADLTAYAAPNRPCGRLSALRIRKTRASEVNAPICIQSPQKQLFQRPH
ALPDHSSLTRIRQRWGAERFRRIFERTVQACVTAKIATGEIVHLDASLIRAHVSWDSLAVRHVEAVVDANDETLRESRQTGRFKKVCVTDPDATMATNGR
NRRLEPAYKQHTAVDDVAGVVVDVEVTTGEENEGMVIAGRLDAAATTTGRAISIATMDAGYAYAKVFRVLEDREIEAIVPAKAEQAPGKVIPTRRFKFDA
RHNLARCPQGKILRPKGKLQRGAFQYFHARAKDCGGCPLRARCVSPSRHARVVVFNVNHPSLLRARRRRIRWGDREERLYQRHRWRVEGVHGEAKTWHGL
ARAVRRGLDNIRIQAFLTAAAINLKRLAVALILLIYADLTAYAAPNRPCGRLSALRIRKTRASEVNAPICIQSPQKQLFQRPH
Blast result :Comments : No STOP codon. The transposase use the first STOP codon in the insertion site.
Comments
References
1] Chmielowska C., Szuplewska M., Bartosik D. (2018) Direct submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.
2] Goldberg,B., Campos,J., Tallon,L., Sadzewicz,L., Sengamalay,N., Ott,S., Godinez,A., Nagaraj,S., Vavikolanu,K., Aluvathingal,J., Nadendla,S. and Sichtig,H. (2017) Direct GenBank submission.