ISBli17
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP017150 | ND | Brevibacterium linens | Brevibacterium linens SMQ-1335 Brevibacterium linens Brevibacterium aurantiacum |
DNA section
IS Length : 1293 bp
Ends
IR Length : 22/25
IRL : GGGACTGATGCAGGGTTAGGTGACATCTAATCTGGCTTGTTGACAGGCAA
IRR : GGGACTGATGCAAGGAGAGGTGACAATTCAAGTGTCACGCTGCGAGTGCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCGCTCCGCACTTC | TGAGCCGGTAGTCGG | 0 | |
| CCATAGGAGCCG | GTG | CATTCGATGCCC | 3 |
DNA sequence
GGGACTGATGCAGGGTTAGGTGACATCTAATCTGGCTTGTTGACAGGCAAGCCTGGAAGGATAGACACCATGCCACAGCCCTACCCGAAAGAATTCCGCG
ACGACGTCATCCAGGTCGCTTTGAACCGGGACTCGAAGACGACGATCGCTCAGATCGCCAAGGACTTCGGGATCCATGAAGGCACCCTGAACAAATGGAT
CCGCCAGGCCGAGATCGATGCCGGCAACAAACCTGGCAAGTCCACCGACGAATCAGCAGAACTGCGAGAACTACGACGGCGGACTCGCCTGCTGGAGCAA
GAGAACGAGGTCCTGCGCCGTGCTGCCGCGTATCTGTCACAGGCGAACCTGCCGTCAAAAGGCTCTACCCGCTCGTGAGAGAGCTCGCCGCCGACGGGAT
CCCCGTCGCGGTGTCGTGCCGGGTCTTGAAGCTCTCACGCCAGCCCTACTACCGGTGGCTGGCAGCACCGGTTCCTGAAGCCGAGGTCGCCGAAGCCTAT
CGGGCCAACGCACTCTTCGACGCCCACCGCGACGACCCGGAATTCGGATACCGGTACCTCGCAGATGAGGCGGAGTCGGCAGGCCAGCCTATGGCTGTTC
GCACTGCGTGGCGGTTGTGTTCGTCCAATGCCTGGTTCTCGGCCTTCGGCAAGGGGCGGGCGAAGAACGGGAAGAAGCCGGGACCACCGGTTCACGACGA
TCTATGCGCGTTTGTCGATGCCGACGGACGGACCAGACACGAGTTCCATGCTGATGCCCCGAATCAGCTGTGGTTAACCGACATCTCGGAACACCACACG
GGTGAGGGCAAGCTGTACCTCTGCGCCGTCAAAGACGCCTACTCCTCGCGCATCGTCGGATATTCGATCAGTTCGAGGATGAAGGCCCGGTTGGCTGTTG
ATGCCCTCGAGATGGCTGTGGCCAGGCGCGGTGATGTGGCCGGATGCAAGACGCACAGCGACCGCGGCAGCCAATTCCGGTCGAAGAAATTCACTCGGGC
TTTGGCCCGTCATGGCATCATCGGGTCGATGGGCAGAGTCGGCGCGGCTGGCGACAACGCAGCGATGGAGAGCTTCTTTGCGCTGTTGCAGAAGAACGTT
CTCGACCGCCGCCGATGGGCGACCCGTGACCAACTGAGAATCGCGATAGTGACGTGGATCGAGCGGACGTATCATCGACGTCGCCGACAAGCTCGGTTGG
GTCGTTTGACACCGATTGAGTACGAAGCGATCATTGAGTCACCTGCACTCGCAGCGTGACACTTGAATTGTCACCTCTCCTTGCATCAGTCCC
ACGACGTCATCCAGGTCGCTTTGAACCGGGACTCGAAGACGACGATCGCTCAGATCGCCAAGGACTTCGGGATCCATGAAGGCACCCTGAACAAATGGAT
CCGCCAGGCCGAGATCGATGCCGGCAACAAACCTGGCAAGTCCACCGACGAATCAGCAGAACTGCGAGAACTACGACGGCGGACTCGCCTGCTGGAGCAA
GAGAACGAGGTCCTGCGCCGTGCTGCCGCGTATCTGTCACAGGCGAACCTGCCGTCAAAAGGCTCTACCCGCTCGTGAGAGAGCTCGCCGCCGACGGGAT
CCCCGTCGCGGTGTCGTGCCGGGTCTTGAAGCTCTCACGCCAGCCCTACTACCGGTGGCTGGCAGCACCGGTTCCTGAAGCCGAGGTCGCCGAAGCCTAT
CGGGCCAACGCACTCTTCGACGCCCACCGCGACGACCCGGAATTCGGATACCGGTACCTCGCAGATGAGGCGGAGTCGGCAGGCCAGCCTATGGCTGTTC
GCACTGCGTGGCGGTTGTGTTCGTCCAATGCCTGGTTCTCGGCCTTCGGCAAGGGGCGGGCGAAGAACGGGAAGAAGCCGGGACCACCGGTTCACGACGA
TCTATGCGCGTTTGTCGATGCCGACGGACGGACCAGACACGAGTTCCATGCTGATGCCCCGAATCAGCTGTGGTTAACCGACATCTCGGAACACCACACG
GGTGAGGGCAAGCTGTACCTCTGCGCCGTCAAAGACGCCTACTCCTCGCGCATCGTCGGATATTCGATCAGTTCGAGGATGAAGGCCCGGTTGGCTGTTG
ATGCCCTCGAGATGGCTGTGGCCAGGCGCGGTGATGTGGCCGGATGCAAGACGCACAGCGACCGCGGCAGCCAATTCCGGTCGAAGAAATTCACTCGGGC
TTTGGCCCGTCATGGCATCATCGGGTCGATGGGCAGAGTCGGCGCGGCTGGCGACAACGCAGCGATGGAGAGCTTCTTTGCGCTGTTGCAGAAGAACGTT
CTCGACCGCCGCCGATGGGCGACCCGTGACCAACTGAGAATCGCGATAGTGACGTGGATCGAGCGGACGTATCATCGACGTCGCCGACAAGCTCGGTTGG
GTCGTTTGACACCGATTGAGTACGAAGCGATCATTGAGTCACCTGCACTCGCAGCGTGACACTTGAATTGTCACCTCTCCTTGCATCAGTCCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 70 | 378 | + | No |
Description : First part of the transposase
ORF sequence :
MPQPYPKEFRDDVIQVALNRDSKTTIAQIAKDFGIHEGTLNKWIRQAEIDAGNKPGKSTDESAELRELRRRTRLLEQENEVLRRAAAYLSQANLPSKGST
RS
RS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1080 bp | 359 aa | 180 | 1259 | + | No |
Description : Second part of the transposase
ORF sequence :
RHPEQMDPPGRDRCRQQTWQVHRRISRTARTTTADSPAGARERGPAPCCRVSVTGEPAVKRLYPLVRELAADGIPVAVSCRVLKLSRQPYYRWLAAPVPE
AEVAEAYRANALFDAHRDDPEFGYRYLADEAESAGQPMAVRTAWRLCSSNAWFSAFGKGRAKNGKKPGPPVHDDLCAFVDADGRTRHEFHADAPNQLWLT
DISEHHTGEGKLYLCAVKDAYSSRIVGYSISSRMKARLAVDALEMAVARRGDVAGCKTHSDRGSQFRSKKFTRALARHGIIGSMGRVGAAGDNAAMESFF
ALLQKNVLDRRRWATRDQLRIAIVTWIERTYHRRRRQARLGRLTPIEYEAIIESPALAA
AEVAEAYRANALFDAHRDDPEFGYRYLADEAESAGQPMAVRTAWRLCSSNAWFSAFGKGRAKNGKKPGPPVHDDLCAFVDADGRTRHEFHADAPNQLWLT
DISEHHTGEGKLYLCAVKDAYSSRIVGYSISSRMKARLAVDALEMAVARRGDVAGCKTHSDRGSQFRSKKFTRALARHGIIGSMGRVGAAGDNAAMESFF
ALLQKNVLDRRRWATRDQLRIAIVTWIERTYHRRRRQARLGRLTPIEYEAIIESPALAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1190 bp | 397 aa | 70 | 1259 | + | Yes |
Chemistry : DDE
ORF sequence :
MPQPYPKEFRDDVIQVALNRDSKTTIAQIAKDFGIHEGTLNKWIRQAEIDAGNKPGKSTDESAELRELRRRTRLLEQENEVLRRAAAYLSQANLPSKKRL
YPLVRELAADGIPVAVSCRVLKLSRQPYYRWLAAPVPEAEVAEAYRANALFDAHRDDPEFGYRYLADEAESAGQPMAVRTAWRLCSSNAWFSAFGKGRAK
NGKKPGPPVHDDLCAFVDADGRTRHEFHADAPNQLWLTDISEHHTGEGKLYLCAVKDAYSSRIVGYSISSRMKARLAVDALEMAVARRGDVAGCKTHSDR
GSQFRSKKFTRALARHGIIGSMGRVGAAGDNAAMESFFALLQKNVLDRRRWATRDQLRIAIVTWIERTYHRRRRQARLGRLTPIEYEAIIESPALAA
YPLVRELAADGIPVAVSCRVLKLSRQPYYRWLAAPVPEAEVAEAYRANALFDAHRDDPEFGYRYLADEAESAGQPMAVRTAWRLCSSNAWFSAFGKGRAK
NGKKPGPPVHDDLCAFVDADGRTRHEFHADAPNQLWLTDISEHHTGEGKLYLCAVKDAYSSRIVGYSISSRMKARLAVDALEMAVARRGDVAGCKTHSDR
GSQFRSKKFTRALARHGIIGSMGRVGAAGDNAAMESFFALLQKNVLDRRRWATRDQLRIAIVTWIERTYHRRRRQARLGRLTPIEYEAIIESPALAA
Blast result :
Comments
ISBli17 is 95% aa similar to ISBli35.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.
2] de Melo,A.G., Labrie,S.J., Dumaresq,J., Roberts,R.J., Tremblay,D.M. and Moineau,S. (2016) Direct GenBank submission.
2] de Melo,A.G., Labrie,S.J., Dumaresq,J., Roberts,R.J., Tremblay,D.M. and Moineau,S. (2016) Direct GenBank submission.