ISBli32
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP014869.1 | ND | Brevibacterium linens | Brevibacterium linens Brevibacterium linens BS258 Brevibacterium epidermidis BS258 |
DNA section
IS Length : 1596 bp
Ends
IR Length : 35/48
IRL : CGAAAAACGGGCGGAGCGTCGAGAAACGGGTGTGCGCCCTCTGTGTCAGG
IRR : CGAAAAACGCACGGAGCGTCGAGAAACGGGCGCGCACGGTATGTGTCAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCACACCGATTCGT | GCGAACGGCTACCCC | 0 | |
| CTCCTGCCGATTCAT | GCGAGTGATCAGAAC | 0 | |
| CACAGGCCCAGCCGC | ATGAGCTTCGGCAGC | 0 | |
| GCAAGGCTGCTTCAC | CTGATTCGCGGTCTC | 0 | |
| CTGAGGCAGCTTCAT | GTTCAGGGAGGTTGC | 0 |
DNA sequence
CGAAAAACGGGCGGAGCGTCGAGAAACGGGTGTGCGCCCTCTGTGTCAGGCTGTAGTGAGACAGCCTGACACAGTAGGCGGGGAATAGGTGAACACCATG
CCCAAGCAACGACGCCCACGCTTAACCATTAACGAGAAACTCGCCATCCTCGACGAATACAACCTTGTCCCACGCGGGCAGCGAGCACTGTTCTGCCGCG
AACGAGGACTCTCGATATCCGCAGTCCAAGACTGGCAGAAGAAACGCCGCGACGGACTCCTGACACCGACGGATAAGAAAGAGTCACGAATCGCGCTGAC
TCGGGCAGAACGCGTCGACTATGAACAACAACGCCAGAAGATCGCTGACTTAGAAGCTCGACTCGAGCAGTCAGAAAACGCGGTCGAGATCCTGGGAAAA
GCATCCGCGCTCTTGGAAACATTGACCAAGAGCGCCGCGACCAAACAATCCCGGCCAGCACCAGTCCAGGAGCCGGACCCCAACGCGTCGGACCCCGCCT
GGGGCAAGCGATTCAACAGGTCCTAGCAGCAAGTATTGATGCACTCGTTGACATCGGGTGGTCGATTCGCGTCGGGTGTGAGTGTGTGGGGTATCCGCGT
GCCTCGTTTTACCGTCATCACCGCACCACGGCGAAGTCGGCATCAGCACCGATCCCGCAGGCACTGCGTTTCCAACCGAGTGCGTTGACCGATGACGAAC
GAGGTCAGATCCTTCACGAGCTGTCCAAGGACGAGTACGCCAACCTCTCCGTCGGCCAGGTCTTCTACCGAGCCTGGGATGCTGGGTGTTATATCGCTTC
ACGCTCCTCGTGGTACCGGGTCGCTGCCGACCACGGGCTCGTCGGCGACCGGCGCCGGAAGTCGCAGTCACGATCACGACCGGGACGGAAGGAGAAACCC
GAACTCGTCGCGAATGCTCCCGGCCAGGTCTGGTCATGGGACATCACGAAACTATCGACCCCGGTCAGGGGTGTGCACTGGCACCTGTATGTCATCGTCG
ATGTGTATTCCCGATACGTCGTAGCGTGGGAACTCCACTCCTACGAAGACGGTCGGATCGCGACAACGATGATCGAGACAGCGTCGAAAAAGTACGGTCG
GCCCGACTATCTGCATTCGGACAACGGCGCCGCGATGGTGTCGAAGCCGGTATCGCGGTTGTCATCACTGTTGGGCATCGAGTTGTCGTTCTCGCGTCCG
AGCGTGTCGAATGACAACCCGTATTCGGAAGCACTCTTCAAAACGTGTAAGTACGTGTTGGAGTTTCCCGAGCGGTTCGATGACTTCAGTGATGCTCACG
CCTTTTGTGTGTGGTTCTTCGCCATGTATAACACTGAGCACCGTCATTCCGGGATCGGTTACCACACACCGGTCAATGTTCATTTCCGTCGTACCAGTGC
TGTCACGGCTCAGCGGAGGAGACTGTTGGATCAGTCGTATCGGCGTCATCCAGACCGATATGGGCAACGTCCCAGGCCACCGCGTCTACCGGGACGTGCC
CATATCAACCAGCCCATCAAACAACCCACGGAACTGTCTCAAACAAGTTGACACATACCGTGCGCGCCCGTTTCTCGACGCTCCGTGCGTTTTTCG
CCCAAGCAACGACGCCCACGCTTAACCATTAACGAGAAACTCGCCATCCTCGACGAATACAACCTTGTCCCACGCGGGCAGCGAGCACTGTTCTGCCGCG
AACGAGGACTCTCGATATCCGCAGTCCAAGACTGGCAGAAGAAACGCCGCGACGGACTCCTGACACCGACGGATAAGAAAGAGTCACGAATCGCGCTGAC
TCGGGCAGAACGCGTCGACTATGAACAACAACGCCAGAAGATCGCTGACTTAGAAGCTCGACTCGAGCAGTCAGAAAACGCGGTCGAGATCCTGGGAAAA
GCATCCGCGCTCTTGGAAACATTGACCAAGAGCGCCGCGACCAAACAATCCCGGCCAGCACCAGTCCAGGAGCCGGACCCCAACGCGTCGGACCCCGCCT
GGGGCAAGCGATTCAACAGGTCCTAGCAGCAAGTATTGATGCACTCGTTGACATCGGGTGGTCGATTCGCGTCGGGTGTGAGTGTGTGGGGTATCCGCGT
GCCTCGTTTTACCGTCATCACCGCACCACGGCGAAGTCGGCATCAGCACCGATCCCGCAGGCACTGCGTTTCCAACCGAGTGCGTTGACCGATGACGAAC
GAGGTCAGATCCTTCACGAGCTGTCCAAGGACGAGTACGCCAACCTCTCCGTCGGCCAGGTCTTCTACCGAGCCTGGGATGCTGGGTGTTATATCGCTTC
ACGCTCCTCGTGGTACCGGGTCGCTGCCGACCACGGGCTCGTCGGCGACCGGCGCCGGAAGTCGCAGTCACGATCACGACCGGGACGGAAGGAGAAACCC
GAACTCGTCGCGAATGCTCCCGGCCAGGTCTGGTCATGGGACATCACGAAACTATCGACCCCGGTCAGGGGTGTGCACTGGCACCTGTATGTCATCGTCG
ATGTGTATTCCCGATACGTCGTAGCGTGGGAACTCCACTCCTACGAAGACGGTCGGATCGCGACAACGATGATCGAGACAGCGTCGAAAAAGTACGGTCG
GCCCGACTATCTGCATTCGGACAACGGCGCCGCGATGGTGTCGAAGCCGGTATCGCGGTTGTCATCACTGTTGGGCATCGAGTTGTCGTTCTCGCGTCCG
AGCGTGTCGAATGACAACCCGTATTCGGAAGCACTCTTCAAAACGTGTAAGTACGTGTTGGAGTTTCCCGAGCGGTTCGATGACTTCAGTGATGCTCACG
CCTTTTGTGTGTGGTTCTTCGCCATGTATAACACTGAGCACCGTCATTCCGGGATCGGTTACCACACACCGGTCAATGTTCATTTCCGTCGTACCAGTGC
TGTCACGGCTCAGCGGAGGAGACTGTTGGATCAGTCGTATCGGCGTCATCCAGACCGATATGGGCAACGTCCCAGGCCACCGCGTCTACCGGGACGTGCC
CATATCAACCAGCCCATCAAACAACCCACGGAACTGTCTCAAACAAGTTGACACATACCGTGCGCGCCCGTTTCTCGACGCTCCGTGCGTTTTTCG
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 429 bp | 142 aa | 98 | 526 | + | No |
Description : First part of the transposase
ORF sequence :
MPKQRRPRLTINEKLAILDEYNLVPRGQRALFCRERGLSISAVQDWQKKRRDGLLTPTDKKESRIALTRAERVDYEQQRQKIADLEARLEQSENAVEILG
KASALLETLTKSAATKQSRPAPVQEPDPNASDPAWGKRFNRS
KASALLETLTKSAATKQSRPAPVQEPDPNASDPAWGKRFNRS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1203 bp | 400 aa | 349 | 1551 | + | No |
Description : Second part of the transposase
ORF sequence :
LRSSTRAVRKRGRDPGKSIRALGNIDQERRDQTIPASTSPGAGPQRVGPRLGQAIQQVLAASIDALVDIGWSIRVGCECVGYPRASFYRHHRTTAKSASA
PIPQALRFQPSALTDDERGQILHELSKDEYANLSVGQVFYRAWDAGCYIASRSSWYRVAADHGLVGDRRRKSQSRSRPGRKEKPELVANAPGQVWSWDIT
KLSTPVRGVHWHLYVIVDVYSRYVVAWELHSYEDGRIATTMIETASKKYGRPDYLHSDNGAAMVSKPVSRLSSLLGIELSFSRPSVSNDNPYSEALFKTC
KYVLEFPERFDDFSDAHAFCVWFFAMYNTEHRHSGIGYHTPVNVHFRRTSAVTAQRRRLLDQSYRRHPDRYGQRPRPPRLPGRAHINQPIKQPTELSQTS
PIPQALRFQPSALTDDERGQILHELSKDEYANLSVGQVFYRAWDAGCYIASRSSWYRVAADHGLVGDRRRKSQSRSRPGRKEKPELVANAPGQVWSWDIT
KLSTPVRGVHWHLYVIVDVYSRYVVAWELHSYEDGRIATTMIETASKKYGRPDYLHSDNGAAMVSKPVSRLSSLLGIELSFSRPSVSNDNPYSEALFKTC
KYVLEFPERFDDFSDAHAFCVWFFAMYNTEHRHSGIGYHTPVNVHFRRTSAVTAQRRRLLDQSYRRHPDRYGQRPRPPRLPGRAHINQPIKQPTELSQTS
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1454 bp | 485 aa | 98 | 1551 | + | Yes |
Chemistry : DDE
ORF sequence :
MPKQRRPRLTINEKLAILDEYNLVPRGQRALFCRERGLSISAVQDWQKKRRDGLLTPTDKKESRIALTRAERVDYEQQRQKIADLEARLEQSENKRGRDP
GKSIRALGNIDQERRDQTIPASTSPGAGPQRVGPRLGQAIQQVLAASIDALVDIGWSIRVGCECVGYPRASFYRHHRTTAKSASAPIPQALRFQPSALTD
DERGQILHELSKDEYANLSVGQVFYRAWDAGCYIASRSSWYRVAADHGLVGDRRRKSQSRSRPGRKEKPELVANAPGQVWSWDITKLSTPVRGVHWHLYV
IVDVYSRYVVAWELHSYEDGRIATTMIETASKKYGRPDYLHSDNGAAMVSKPVSRLSSLLGIELSFSRPSVSNDNPYSEALFKTCKYVLEFPERFDDFSD
AHAFCVWFFAMYNTEHRHSGIGYHTPVNVHFRRTSAVTAQRRRLLDQSYRRHPDRYGQRPRPPRLPGRAHINQPIKQPTELSQTS
GKSIRALGNIDQERRDQTIPASTSPGAGPQRVGPRLGQAIQQVLAASIDALVDIGWSIRVGCECVGYPRASFYRHHRTTAKSASAPIPQALRFQPSALTD
DERGQILHELSKDEYANLSVGQVFYRAWDAGCYIASRSSWYRVAADHGLVGDRRRKSQSRSRPGRKEKPELVANAPGQVWSWDITKLSTPVRGVHWHLYV
IVDVYSRYVVAWELHSYEDGRIATTMIETASKKYGRPDYLHSDNGAAMVSKPVSRLSSLLGIELSFSRPSVSNDNPYSEALFKTCKYVLEFPERFDDFSD
AHAFCVWFFAMYNTEHRHSGIGYHTPVNVHFRRTSAVTAQRRRLLDQSYRRHPDRYGQRPRPPRLPGRAHINQPIKQPTELSQTS
Blast result :
Comments
ISBli32 is 54% aa similar to ISBli10.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.
2] Zhu,Y. and Sun,C. (2016) Direct GenBank submission.
2] Zhu,Y. and Sun,C. (2016) Direct GenBank submission.