ISBli35
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP014869 | ND | Brevibacterium linens | Brevibacterium linens Brevibacterium linens BS258 Brevibacterium epidermidis BS258 |
DNA section
IS Length : 1296 bp
Ends
IR Length : 21/25
IRL : GGGACTGATGCAGGGTTTGGTGACATCTGATCTGGCTTGTTCACAGGCAA
IRR : GGGACTGATGTAAGGATCGGTGACAATTCGGGTGTCACGCTGCGACTGCA
Comments : Atypical ends for IS3 family member.
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CTCATGACCA | GGG | AGCCAAGGAC | 3 |
DNA sequence
GGGACTGATGCAGGGTTTGGTGACATCTGATCTGGCTTGTTCACAGGCAAGCCTGGAAGGATAGACACCATGCCACAGCCCTACCCGAAAGAGTTCCGCG
ATGACGTCGTCCGTGTCGCATTGAACCGCGACGAGAAGACCACGATTTCCCAAATCGCCAAGGACTTCGGCGTCCACGAAGGCACCATCGCGAAATGGTT
GCGCCAAGCAGATATCGATGCCGGCAACAAGCCGGGCAAGTCCACGGACGAGTCCGCAGAGCTGCGAGAGCTCAGACGTCGGACTCGCCTGCTGGAACAA
GAGAACGAAGTCCTGCGCCGTGCCGCCGCCTATTTGTCGCAGGCGAACCTGCCGTCAAAAGGCTCTACCCGCTCGTAAGAGAGCTCGCCGCCGACGGGAT
CCCCGTCGCGGTGTCGTGCCGGGTCTTGAAGCTCTCCCGTCAGCCGTACTACCGGTGGCTGGCCGCCCCTGTTCCCGAAGCCGATGTCATCGAGGCGTAT
CGGGCCAACGCCCTGTTCGACGCCCACCGGGATGATCCCGAGTTCGGGTACCGGTACCTGGCCGACGAAGCTGAAGCGGTTGGCCAGACGATGGCGGTGC
GCACTGCGTGGCGGTTGTGCTCGCACAACTCGTGGTTCTCGGTCTTTGGCAAGGGCAAGGCGAAGAACGGCAAACGGCCGGGACCACCCGTCCACGACGA
CCTGTGCGCGGTCGTCGACGCCGACGGGCGCACCCGTCACGAGTTCAAAGCTGAGGGTGTGAACCGGCTATGGTTGACCGACATTTCGGAGCACAATACC
GATGAGGGCAAGCTGTACCTCTGTGCGATCAAGGACGCCTACTCAGGGCGGATCGTGGGCTATTCGATCAGCTCGCGGATGAAGGCGCGTTTGGCCGTTG
ATGCCCTCGAGATGGGTGTGGCGCGCAGGGGTGGTGACGTGGCCGGATGCGTGGTCCACAGCGACCGTGGTAGTCAATTCCGGTCGAAGAAATTCACCCG
AGCCTTGGAACGTCACGGCCTAATCGGATCGATGGGCAGAGTCGGTGCTGCTGGCGACAACGCGGCCATGGAATCGTTCTTTGCACTCCTGCAGAAGAAC
GTTCTCGACCGCCGTCGGTGGGCTTCCAGAGGCCAGTTGAGAATCGCGATCGTGACCTGGGTCGAGCGAACGTATCACCGGCGTCGACGGCAAGCTCGGT
TGGGTCGTTTGACACCTGTTGAGTACGAAGCAATTATTGAGTCACCTGCAGTCGCAGCGTGACACCCGAATTGTCACCGATCCTTACATCAGTCCC
ATGACGTCGTCCGTGTCGCATTGAACCGCGACGAGAAGACCACGATTTCCCAAATCGCCAAGGACTTCGGCGTCCACGAAGGCACCATCGCGAAATGGTT
GCGCCAAGCAGATATCGATGCCGGCAACAAGCCGGGCAAGTCCACGGACGAGTCCGCAGAGCTGCGAGAGCTCAGACGTCGGACTCGCCTGCTGGAACAA
GAGAACGAAGTCCTGCGCCGTGCCGCCGCCTATTTGTCGCAGGCGAACCTGCCGTCAAAAGGCTCTACCCGCTCGTAAGAGAGCTCGCCGCCGACGGGAT
CCCCGTCGCGGTGTCGTGCCGGGTCTTGAAGCTCTCCCGTCAGCCGTACTACCGGTGGCTGGCCGCCCCTGTTCCCGAAGCCGATGTCATCGAGGCGTAT
CGGGCCAACGCCCTGTTCGACGCCCACCGGGATGATCCCGAGTTCGGGTACCGGTACCTGGCCGACGAAGCTGAAGCGGTTGGCCAGACGATGGCGGTGC
GCACTGCGTGGCGGTTGTGCTCGCACAACTCGTGGTTCTCGGTCTTTGGCAAGGGCAAGGCGAAGAACGGCAAACGGCCGGGACCACCCGTCCACGACGA
CCTGTGCGCGGTCGTCGACGCCGACGGGCGCACCCGTCACGAGTTCAAAGCTGAGGGTGTGAACCGGCTATGGTTGACCGACATTTCGGAGCACAATACC
GATGAGGGCAAGCTGTACCTCTGTGCGATCAAGGACGCCTACTCAGGGCGGATCGTGGGCTATTCGATCAGCTCGCGGATGAAGGCGCGTTTGGCCGTTG
ATGCCCTCGAGATGGGTGTGGCGCGCAGGGGTGGTGACGTGGCCGGATGCGTGGTCCACAGCGACCGTGGTAGTCAATTCCGGTCGAAGAAATTCACCCG
AGCCTTGGAACGTCACGGCCTAATCGGATCGATGGGCAGAGTCGGTGCTGCTGGCGACAACGCGGCCATGGAATCGTTCTTTGCACTCCTGCAGAAGAAC
GTTCTCGACCGCCGTCGGTGGGCTTCCAGAGGCCAGTTGAGAATCGCGATCGTGACCTGGGTCGAGCGAACGTATCACCGGCGTCGACGGCAAGCTCGGT
TGGGTCGTTTGACACCTGTTGAGTACGAAGCAATTATTGAGTCACCTGCAGTCGCAGCGTGACACCCGAATTGTCACCGATCCTTACATCAGTCCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 309 bp | 102 aa | 70 | 378 | + | No |
Description : First part of the transposase
ORF sequence :
MPQPYPKEFRDDVVRVALNRDEKTTISQIAKDFGVHEGTIAKWLRQADIDAGNKPGKSTDESAELRELRRRTRLLEQENEVLRRAAAYLSQANLPSKGST
RS
RS
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1158 bp | 385 aa | 105 | 1262 | + | No |
Description : Second part of the transposase
ORF sequence :
RRPCRIEPRREDHDFPNRQGLRRPRRHHREMVAPSRYRCRQQAGQVHGRVRRAARAQTSDSPAGTRERSPAPCRRLFVAGEPAVKRLYPLVRELAADGIP
VAVSCRVLKLSRQPYYRWLAAPVPEADVIEAYRANALFDAHRDDPEFGYRYLADEAEAVGQTMAVRTAWRLCSHNSWFSVFGKGKAKNGKRPGPPVHDDL
CAVVDADGRTRHEFKAEGVNRLWLTDISEHNTDEGKLYLCAIKDAYSGRIVGYSISSRMKARLAVDALEMGVARRGGDVAGCVVHSDRGSQFRSKKFTRA
LERHGLIGSMGRVGAAGDNAAMESFFALLQKNVLDRRRWASRGQLRIAIVTWVERTYHRRRRQARLGRLTPVEYEAIIESPAVAA
VAVSCRVLKLSRQPYYRWLAAPVPEADVIEAYRANALFDAHRDDPEFGYRYLADEAEAVGQTMAVRTAWRLCSHNSWFSVFGKGKAKNGKRPGPPVHDDL
CAVVDADGRTRHEFKAEGVNRLWLTDISEHNTDEGKLYLCAIKDAYSGRIVGYSISSRMKARLAVDALEMGVARRGGDVAGCVVHSDRGSQFRSKKFTRA
LERHGLIGSMGRVGAAGDNAAMESFFALLQKNVLDRRRWASRGQLRIAIVTWVERTYHRRRRQARLGRLTPVEYEAIIESPAVAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1193 bp | 398 aa | 70 | 1262 | + | Yes |
Chemistry : DDE
ORF sequence :
MPQPYPKEFRDDVVRVALNRDEKTTISQIAKDFGVHEGTIAKWLRQADIDAGNKPGKSTDESAELRELRRRTRLLEQENEVLRRAAAYLSQANLPSKKRL
YPLVRELAADGIPVAVSCRVLKLSRQPYYRWLAAPVPEADVIEAYRANALFDAHRDDPEFGYRYLADEAEAVGQTMAVRTAWRLCSHNSWFSVFGKGKAK
NGKRPGPPVHDDLCAVVDADGRTRHEFKAEGVNRLWLTDISEHNTDEGKLYLCAIKDAYSGRIVGYSISSRMKARLAVDALEMGVARRGGDVAGCVVHSD
RGSQFRSKKFTRALERHGLIGSMGRVGAAGDNAAMESFFALLQKNVLDRRRWASRGQLRIAIVTWVERTYHRRRRQARLGRLTPVEYEAIIESPAVAA
YPLVRELAADGIPVAVSCRVLKLSRQPYYRWLAAPVPEADVIEAYRANALFDAHRDDPEFGYRYLADEAEAVGQTMAVRTAWRLCSHNSWFSVFGKGKAK
NGKRPGPPVHDDLCAVVDADGRTRHEFKAEGVNRLWLTDISEHNTDEGKLYLCAIKDAYSGRIVGYSISSRMKARLAVDALEMGVARRGGDVAGCVVHSD
RGSQFRSKKFTRALERHGLIGSMGRVGAAGDNAAMESFFALLQKNVLDRRRWASRGQLRIAIVTWVERTYHRRRRQARLGRLTPVEYEAIIESPAVAA
Blast result :
Comments
ISBli35 is 70% aa similar to ISAcl2.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.
2] Zhu,Y. and Sun,C. (2016) Direct GenBank submission.
2] Zhu,Y. and Sun,C. (2016) Direct GenBank submission.