ISEcret9
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KP890203 | ND | Endozoicomonas endosymbiont | Endozoicomonas endosymbiont of Diplodus puntazzo |
DNA section
IS Length : 791 bp
Ends
IR Length : 21/23
IRL : GGTGAAGGTTCAGAAGAGTTGATGGCTATTAATTAAACTGAAGTCTGCTA
IRR : GGTGGAGGTTCAGAGGAGTTGATAGTGCTCTCGGTAGATAAATTCTCCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGATCAAACTGTT | CATTATTAGAGG | 0 |
DNA sequence
GGTGAAGGTTCAGAAGAGTTGATGGCTATTAATTAAACTGAAGTCTGCTACTATGCCAGTCTTTTGTTCTGTACTGCCATGGCTTTTATTGAAGTTGTCT
GTATTCACTGTCGTAAACCTGATGATATTGTCAGAAATGGAAAGGCCAGATCAGGGCTCCAAAGGTTCCTGTGCCGAAGTTGCAACAAGACCTTTCAGCT
GGATTATCTCTACAACGCTAACCAACCAGGTATACATGAAAGGATTGTTCGGCTAACCCATAATGGCTCTGGTGTCAGAGACATTGAGCGATACCTCAAT
ATCAGCCGAACTACTGTTATCGCCCACTTAAAAGACTATCGCCGCCAGCAGTAACAGAGTTTCCATTCGAGAATGCGAAGGTTAAGTTGATCTGCGAGAT
GGATGAACAGTGGTCATTCGTGGGCAGTAAGAAAAATCAACGCTGGCTCTGGTATGCCTGGGAACCACGCTTCAAGCGGATAATTGCCCATGCATTTGGT
CGCCGGACGGATGCGACACTGAAAAAGCTACTGAAATTGCTGGAACCCTATCGATTTGAGTTTTTCTGTACGGATGACTGGGCATCCTATGCGAAGCTGT
TACCAGAGAAAAAACATATTATTGGTAAGCATTTTACTCAGCGTATAGAGAGGCAGAACCTGAATTTCAGGACACGTCTGAAGAGGTTGGCTCGACGATC
TATTTGCTTTTCAAGATCAGAAGAAGTTCACGATAAAGTAATCGGAGAATTTATCTACCGAGAGCACTATCAACTCCTCTGAACCTCCACC
GTATTCACTGTCGTAAACCTGATGATATTGTCAGAAATGGAAAGGCCAGATCAGGGCTCCAAAGGTTCCTGTGCCGAAGTTGCAACAAGACCTTTCAGCT
GGATTATCTCTACAACGCTAACCAACCAGGTATACATGAAAGGATTGTTCGGCTAACCCATAATGGCTCTGGTGTCAGAGACATTGAGCGATACCTCAAT
ATCAGCCGAACTACTGTTATCGCCCACTTAAAAGACTATCGCCGCCAGCAGTAACAGAGTTTCCATTCGAGAATGCGAAGGTTAAGTTGATCTGCGAGAT
GGATGAACAGTGGTCATTCGTGGGCAGTAAGAAAAATCAACGCTGGCTCTGGTATGCCTGGGAACCACGCTTCAAGCGGATAATTGCCCATGCATTTGGT
CGCCGGACGGATGCGACACTGAAAAAGCTACTGAAATTGCTGGAACCCTATCGATTTGAGTTTTTCTGTACGGATGACTGGGCATCCTATGCGAAGCTGT
TACCAGAGAAAAAACATATTATTGGTAAGCATTTTACTCAGCGTATAGAGAGGCAGAACCTGAATTTCAGGACACGTCTGAAGAGGTTGGCTCGACGATC
TATTTGCTTTTCAAGATCAGAAGAAGTTCACGATAAAGTAATCGGAGAATTTATCTACCGAGAGCACTATCAACTCCTCTGAACCTCCACC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 79 | 354 | + | No |
Description : First part of the transposase
ORF sequence :
MAFIEVVCIHCRKPDDIVRNGKARSGLQRFLCRSCNKTFQLDYLYNANQPGIHERIVRLTHNGSGVRDIERYLNISRTTVIAHLKDYRRQQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 495 bp | 164 aa | 288 | 782 | + | No |
Description : Second part of the transposase
ORF sequence :
AIPQYQPNYCYRPLKRLSPPAVTEFPFENAKVKLICEMDEQWSFVGSKKNQRWLWYAWEPRFKRIIAHAFGRRTDATLKKLLKLLEPYRFEFFCTDDWAS
YAKLLPEKKHIIGKHFTQRIERQNLNFRTRLKRLARRSICFSRSEEVHDKVIGEFIYREHYQLL
YAKLLPEKKHIIGKHFTQRIERQNLNFRTRLKRLARRSICFSRSEEVHDKVIGEFIYREHYQLL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 704 bp | 234 aa | 79 | 782 | + | Yes |
Chemistry : DDE
ORF sequence :
MAFIEVVCIHCRKPDDIVRNGKARSGLQRFLCRSCNKTFQLDYLYNANQPGIHERIVRLTHNGSGVRDIERYLNISRTTVIAHLKRLSPPAVTEFPFENA
KVKLICEMDEQWSFVGSKKNQRWLWYAWEPRFKRIIAHAFGRRTDATLKKLLKLLEPYRFEFFCTDDWASYAKLLPEKKHIIGKHFTQRIERQNLNFRTR
LKRLARRSICFSRSEEVHDKVIGEFIYREHYQLL
KVKLICEMDEQWSFVGSKKNQRWLWYAWEPRFKRIIAHAFGRRTDATLKKLLKLLEPYRFEFFCTDDWASYAKLLPEKKHIIGKHFTQRIERQNLNFRTR
LKRLARRSICFSRSEEVHDKVIGEFIYREHYQLL
Blast result :
Comments
ISEcret9 is 73% aa similar to ISYps7.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Helena Seth-Smith (2015) Direct submission.