ISEcret4
- Family IS1
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| KP890198 | ND | Endozoicomonas endosymbiont | Endozoicomonas endosymbiont of Diplodus puntazzo Endozoicomonas atrinae WP70 |
DNA section
IS Length : 766 bp
Ends
IR Length : 20/23
IRL : GGTGATGTGTCAGAGCTGTTGATCTGATACACTCCTGTCTTTTTGAGTCA
IRR : GGTGATGTATCAAAACTGTTGATAGTGGTTTCGGGAGATAAACTCCCCGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCGCCGTAAATGGTATCGGC | TTTGTGAGAAGATTACCTGT | 0 | |
| TAATAAGTCA | GATTAAGG | ATGGATATAC | 8 |
DNA sequence
GGTGATGTGTCAGAGCTGTTGATCTGATACACTCCTGTCTTTTTGAGTCATCTATGGCTGTAGTTCAAGTTGCCTGTATCCATTGTCAACAAACGTTCAA
TGTTGTTAAGAACGGGAAAGCTGAGACGGGTCACCAGCGGTATAGATGCAAGGACTGTAATAAGAGTTTCCAACTTGAGTATCGCTATAACGGCAATCTG
CCTGGTACTCATGAGCAGATCATAAAGATGACCATGAATGGCTCCGGGGTCAGAGATATCGGACGTGTCCTTGAGATCAGTCCAACGACGGTATTGGCTC
GCTTAAAAAACTCGACCCGCCAACAGTAACACAACTCCCCTTCGAAAATGCTCAAGTCAAGATTATCTTCGAAATGGATGAGCAGTGGTCATTTGTTGGT
AACAAGAAGCAGCAACGCTGGCTGTTTTATGCATGGGAGCCACGTTTCAAGAAGATCATTGCCCATGCGTTCGGCTCCAGAAGCAAAGAGACGCTCAAAA
AGCTGTTGGAGCGTCTGGCCCGCTTCAAAGTCAGTTTTTACTGCACAGATGACTGGCAGCCATATACATCCTGTTTACCAGAAGATAAACATATCATTGG
AAAATACTTCACTCAGCGGATTGAGCGGCAGAATCTGACCTTGAGAACTTGCTTGAAACGGTTAGCCAGAAGGACTATCTGCTTCTCAAGATCAATAGAC
ATTCATGATAAAGTTATCGGGGAGTTTATCTCCCGAAACCACTATCAACAGTTTTGATACATCACC
TGTTGTTAAGAACGGGAAAGCTGAGACGGGTCACCAGCGGTATAGATGCAAGGACTGTAATAAGAGTTTCCAACTTGAGTATCGCTATAACGGCAATCTG
CCTGGTACTCATGAGCAGATCATAAAGATGACCATGAATGGCTCCGGGGTCAGAGATATCGGACGTGTCCTTGAGATCAGTCCAACGACGGTATTGGCTC
GCTTAAAAAACTCGACCCGCCAACAGTAACACAACTCCCCTTCGAAAATGCTCAAGTCAAGATTATCTTCGAAATGGATGAGCAGTGGTCATTTGTTGGT
AACAAGAAGCAGCAACGCTGGCTGTTTTATGCATGGGAGCCACGTTTCAAGAAGATCATTGCCCATGCGTTCGGCTCCAGAAGCAAAGAGACGCTCAAAA
AGCTGTTGGAGCGTCTGGCCCGCTTCAAAGTCAGTTTTTACTGCACAGATGACTGGCAGCCATATACATCCTGTTTACCAGAAGATAAACATATCATTGG
AAAATACTTCACTCAGCGGATTGAGCGGCAGAATCTGACCTTGAGAACTTGCTTGAAACGGTTAGCCAGAAGGACTATCTGCTTCTCAAGATCAATAGAC
ATTCATGATAAAGTTATCGGGGAGTTTATCTCCCGAAACCACTATCAACAGTTTTGATACATCACC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 276 bp | 91 aa | 54 | 329 | + | No |
Description : First part of the transposase
ORF sequence :
MAVVQVACIHCQQTFNVVKNGKAETGHQRYRCKDCNKSFQLEYRYNGNLPGTHEQIIKMTMNGSGVRDIGRVLEISPTTVLARLKNSTRQQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 483 bp | 160 aa | 275 | 757 | + | No |
Description : Second part of the transposase
ORF sequence :
DQSNDGIGSLKKLDPPTVTQLPFENAQVKIIFEMDEQWSFVGNKKQQRWLFYAWEPRFKKIIAHAFGSRSKETLKKLLERLARFKVSFYCTDDWQPYTSC
LPEDKHIIGKYFTQRIERQNLTLRTCLKRLARRTICFSRSIDIHDKVIGEFISRNHYQQF
LPEDKHIIGKYFTQRIERQNLTLRTCLKRLARRTICFSRSIDIHDKVIGEFISRNHYQQF
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 704 bp | 234 aa | 54 | 757 | + | Yes |
Chemistry : DDE
ORF sequence :
MAVVQVACIHCQQTFNVVKNGKAETGHQRYRCKDCNKSFQLEYRYNGNLPGTHEQIIKMTMNGSGVRDIGRVLEISPTTVLARLKKLDPPTVTQLPFENA
QVKIIFEMDEQWSFVGNKKQQRWLFYAWEPRFKKIIAHAFGSRSKETLKKLLERLARFKVSFYCTDDWQPYTSCLPEDKHIIGKYFTQRIERQNLTLRTC
LKRLARRTICFSRSIDIHDKVIGEFISRNHYQQF
QVKIIFEMDEQWSFVGNKKQQRWLFYAWEPRFKKIIAHAFGSRSKETLKKLLERLARFKVSFYCTDDWQPYTSCLPEDKHIIGKYFTQRIERQNLTLRTC
LKRLARRTICFSRSIDIHDKVIGEFISRNHYQQF
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISEcret4 orfAB is 70% aa similar to ISYps7.
ISEcret4 orfAB is 70% aa similar to ISYps7.
References
1] Helena Seth-Smith (2015) Direct submission.