ISBli28
- Family IS3
- Group IS51
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Brevibacterium linens | Brevibacterium linens Brevibacterium linens ATCC 19391 |
DNA section
IS Length : 1350 bp
Ends
IR Length : 17/25
IRL : TGATTCGCCCCGGGAATAATGGAGACAGTGAAGCCACGGAAAGGACTTCC
IRR : TGTCAAGCCCCGTGTTTGATGGAGAGTTTTCTACTTGGAATTCATCGGCT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GCTGCTTCAGCA | ATGA | TGGCGGCATCACGGGC | 4 |
| TCAGCACCATCA | CCT | CGAAGATCACGA | 3 |
| AGACGATCGTCG | ACA | TCGTCGCCGAGG | 3 |
| GGCAAGGCGGAGTTT | GCGCTGCCAATAGAC | 0 |
DNA sequence
TGATTCGCCCCGGGAATAATGGAGACAGTGAAGCCACGGAAAGGACTTCCCTGTCATGCCCCAAAAGAAGTACCCCCTCGAGCTACGCGAACGAGCAGTC
CGCATGGTCCTCGACGCCCTCGAAGCTGACCCTGGTGCCCGCCGCGGCGTCTTCCGCCGGATCGGCGACCAACTCGGTGTGAACCCAGAAACACTGCGGT
CCTGGGTCAATCAGGACCAGGTCGATGCCGGCGACCGCCCCGGCACTACCACCGCTGAAGCCCAGCGCATCAAGGACCTCGAGAAGGAGAATCGGGAACT
GCGCCGGGCCAACCAAATCCTGAAGTCGGCCTCGGCTTTTTTCGCGGCGGAGCTCGACCGCCCCTCGAAGTAGTCTGCTCGTATATCGACGAGCACAAGG
ACGAACTCGGAGTCGAGCCGATCTGTGCCACGCTGCGTGCTGCCGGCGTGAAGATCGCTCCGAGTACCTACTATGCCCGCAAACATCGACCAGCCTCGGC
TCGACAGAAACGTGACGAGGTATTGAAAGAAGAGATCAGCACTGTGCACGGGAAGAATTACGATGCGTTCGGAGCCAGGAAGATGCATATCGTGCTCAAC
CGCGAACCTGACTCGCATGGTCGCGGGCATGTGGCCCGTTGCACGATCGAGCGCCTGATGAGAGACCTCGATCTACACGGGATCAAGAGGGCCAAGGCAC
CGAACACTACGAGGTCAGCACCGCGGGAGAACTGCCCGGCCGATCTCGTCGATCGGCACTTCGAAGCCTTCGCTCCTGACCGGCTGTGGGTGGCCGATAT
CACCTATGTGCACACGTTCACAGGGTGGGTCTACGTCGCGTTCGTCACCGACGTGTTCAATCGCGAGATCGTCGGCTGGCAGGTCTCGAGGTCGTTGCAT
ACCGAGTTGGCTCTTGACGCTTTGCAGATGGGGATCTATCAGCGTAAACGTGCTGGTCACGACCTGTCTGGGCTGGTGCATCACTCGGACCGCGGGGTCC
AGTATCGAGCGATTCGCTATGGCGAGGCCCTGGCTGAGCAAGATGCAGTCGCGTCTGTCGGGTCGAAAGGAGATTCATACGATAACGCCCTCGCTGAAGC
GCTGAATTCACTCTATAAGGCCGAACTCATCCGGAACAAGGGTCCCTGGAAAGGTATTGATGATGTCGAGATCGCCACGGCGCAGTGGGTGCACTGGTTC
AACACGTACCGGCCCCACGGAGCACTTGGTGGGGCGACTCCCGCCGCGTTCCGAGCCGATCATCTCAGCACGATGCCACGGGCTGCGTAGCTCGTGGGTG
AGCCGATGAATTCCAAGTAGAAAACTCTCCATCAAACACGGGGCTTGACA
CGCATGGTCCTCGACGCCCTCGAAGCTGACCCTGGTGCCCGCCGCGGCGTCTTCCGCCGGATCGGCGACCAACTCGGTGTGAACCCAGAAACACTGCGGT
CCTGGGTCAATCAGGACCAGGTCGATGCCGGCGACCGCCCCGGCACTACCACCGCTGAAGCCCAGCGCATCAAGGACCTCGAGAAGGAGAATCGGGAACT
GCGCCGGGCCAACCAAATCCTGAAGTCGGCCTCGGCTTTTTTCGCGGCGGAGCTCGACCGCCCCTCGAAGTAGTCTGCTCGTATATCGACGAGCACAAGG
ACGAACTCGGAGTCGAGCCGATCTGTGCCACGCTGCGTGCTGCCGGCGTGAAGATCGCTCCGAGTACCTACTATGCCCGCAAACATCGACCAGCCTCGGC
TCGACAGAAACGTGACGAGGTATTGAAAGAAGAGATCAGCACTGTGCACGGGAAGAATTACGATGCGTTCGGAGCCAGGAAGATGCATATCGTGCTCAAC
CGCGAACCTGACTCGCATGGTCGCGGGCATGTGGCCCGTTGCACGATCGAGCGCCTGATGAGAGACCTCGATCTACACGGGATCAAGAGGGCCAAGGCAC
CGAACACTACGAGGTCAGCACCGCGGGAGAACTGCCCGGCCGATCTCGTCGATCGGCACTTCGAAGCCTTCGCTCCTGACCGGCTGTGGGTGGCCGATAT
CACCTATGTGCACACGTTCACAGGGTGGGTCTACGTCGCGTTCGTCACCGACGTGTTCAATCGCGAGATCGTCGGCTGGCAGGTCTCGAGGTCGTTGCAT
ACCGAGTTGGCTCTTGACGCTTTGCAGATGGGGATCTATCAGCGTAAACGTGCTGGTCACGACCTGTCTGGGCTGGTGCATCACTCGGACCGCGGGGTCC
AGTATCGAGCGATTCGCTATGGCGAGGCCCTGGCTGAGCAAGATGCAGTCGCGTCTGTCGGGTCGAAAGGAGATTCATACGATAACGCCCTCGCTGAAGC
GCTGAATTCACTCTATAAGGCCGAACTCATCCGGAACAAGGGTCCCTGGAAAGGTATTGATGATGTCGAGATCGCCACGGCGCAGTGGGTGCACTGGTTC
AACACGTACCGGCCCCACGGAGCACTTGGTGGGGCGACTCCCGCCGCGTTCCGAGCCGATCATCTCAGCACGATGCCACGGGCTGCGTAGCTCGTGGGTG
AGCCGATGAATTCCAAGTAGAAAACTCTCCATCAAACACGGGGCTTGACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 318 bp | 105 aa | 56 | 373 | + | No |
Description : First part of the transposase
ORF sequence :
MPQKKYPLELRERAVRMVLDALEADPGARRGVFRRIGDQLGVNPETLRSWVNQDQVDAGDRPGTTTAEAQRIKDLEKENRELRRANQILKSASAFFAAEL
DRPSK
DRPSK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 972 bp | 343 aa | 259 | 1230 | + | No |
Description : Second part of the transposase
ORF sequence :
SPAHQGPREGESGTAPGQPNPEVGLGFFRGGARPPLEVVCSYIDEHKDELGVEPICATLRAAGVKIAPSTYYARKHRPASARQKRDEVLKEEISTVHGKN
YDAFGARKMHIVLNREPDSHGRGHVARCTIERLMRDLDLHGIKRAKAPNTTRSAPRENCPADLVDRHFEAFAPDRLWVADITYVHTFTGWVYVAFVTDVF
NREIVGWQVSRSLHTELALDALQMGIYQRKRAGHDLSGLVHHSDRGVQYRAIRYGEALAEQDAVASVGSKGDSYDNALAEALNSLYKAELIRNKGPWKGI
DDVEIATAQWVHWFNTYRPHGALGGATPAAFRADHLSTMPRAA
YDAFGARKMHIVLNREPDSHGRGHVARCTIERLMRDLDLHGIKRAKAPNTTRSAPRENCPADLVDRHFEAFAPDRLWVADITYVHTFTGWVYVAFVTDVF
NREIVGWQVSRSLHTELALDALQMGIYQRKRAGHDLSGLVHHSDRGVQYRAIRYGEALAEQDAVASVGSKGDSYDNALAEALNSLYKAELIRNKGPWKGI
DDVEIATAQWVHWFNTYRPHGALGGATPAAFRADHLSTMPRAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1175 bp | 411 aa | 56 | 1230 | + | Yes |
Description : Second part of the transposase
ORF sequence :
MPQKKYPLELRERAVRMVLDALEADPGARRGVFRRIGDQLGVNPETLRSWVNQDQVDAGDRPGTTTAEAQRIKDLEKENRELRRANQILKSASAFFRGGA
RPPLEVVCSYIDEHKDELGVEPICATLRAAGVKIAPSTYYARKHRPASARQKRDEVLKEEISTVHGKNYDAFGARKMHIVLNREPDSHGRGHVARCTIER
LMRDLDLHGIKRAKAPNTTRSAPRENCPADLVDRHFEAFAPDRLWVADITYVHTFTGWVYVAFVTDVFNREIVGWQVSRSLHTELALDALQMGIYQRKRA
GHDLSGLVHHSDRGVQYRAIRYGEALAEQDAVASVGSKGDSYDNALAEALNSLYKAELIRNKGPWKGIDDVEIATAQWVHWFNTYRPHGALGGATPAAFR
ADHLSTMPRAA
RPPLEVVCSYIDEHKDELGVEPICATLRAAGVKIAPSTYYARKHRPASARQKRDEVLKEEISTVHGKNYDAFGARKMHIVLNREPDSHGRGHVARCTIER
LMRDLDLHGIKRAKAPNTTRSAPRENCPADLVDRHFEAFAPDRLWVADITYVHTFTGWVYVAFVTDVFNREIVGWQVSRSLHTELALDALQMGIYQRKRA
GHDLSGLVHHSDRGVQYRAIRYGEALAEQDAVASVGSKGDSYDNALAEALNSLYKAELIRNKGPWKGIDDVEIATAQWVHWFNTYRPHGALGGATPAAFR
ADHLSTMPRAA
Blast result :
Comments
ISBli28 is 66% aa similar to ISBli25.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.