ISPa75

  • Family IS66
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MF144194 ND Pseudomonas aeruginosa
Pseudomonas aeruginosa 1160 plasmid p1160-VIM
DNA section
IS Length : 2966 bp

Ends


IR Length : 23/33

IRL : GTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATCA
IRR : GTAACCGTCCGGGCACCCCACCTTTACGATCTCTTGGGTTGATGATTCTG

Insertion site


Left flankDirect repeatRight flankDR Length
AGAGGGAACCTATTTCGATGCGCCGACAGTGG8

DNA sequence

GTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATCACCCTTATCGCCAGGATTTTCATGCGCCAACGAAGCTCTTATCCGAAACCA
TTCAAAGCTCAGGTCGTTCAGGAATGCCTGCAACCTGGCGCGACGGTGTCCAGTGTCGCCATCAGCCACGGCATCAATGCCAACGTCATCCGCAAATGGA
TGCCGCTCTATCGAGATCAGCCCGCAGCGACCTCACTGCCGGCCTTCGTCCCGCTAAAAGCCGCACCTAAACGGCCAGTCGAAATGTCAGTGATCATCGA
ATTGCCCATGGCCGGGCAAGTGATCACGGTGAAATGGCCAACCTCAGATCCCGAGGGATGCGCGCAATTTATCCGGGCTGTCGCTCAATGATCCGCATTG
ATGCGATCTGGCTGGCCACCGAGCCGATGGACATGCGCGCCGGTACCGAAACCGCATTAGCCCGGGTGATCGCGGTGTTTGGTGCGGCGAAGCCGCACTG
CGCTTATCTGTTCGCCAATCGTCGGGCTAACCGGATGAAAGTGCTAGTGCACGATGGCGTCGGCGTCTGGCTTGCAGCGCGTCGACTGAACCAAGGCAAG
TTCCACTGGCCCGGTATTCGCAATGGCTGCGAGGTCGAACTCGATAACGACCAACTTCAGGCGTTAGTTCTGGGCCTGCCGTGGCAGCGTGTTGGCGCCG
GCGGTGTGATTAGCATGCTGTAAACGCCGGCCATTTGGCCGGTCTACTTGTCGTCGCCCTGCGCCTGCTCTGGCACAATCGGCGGCATGACTTTCTCGCC
CAACCTCGACCAGATGACCCCGGAACAGCTTCGCGCCTTGGCGGCACAGGCGTTGCAGTTGCAGTCCCAGGTCGAGGCGATGAGCAGGAAGATCCAAAAC
GATCAAACCGTCATTGAGCAGCTCACTTACGAAATTGCCCTGCTCAAACGCCACAAGTTTGCCAAGCGCAGCGAGCAAATCAGCCCGGCGCAAAGCAGCT
TGCTGGATGACCTGCTCGACACAGACCTTGAAGCTATTGAGGCCGAGCTAAAACAGCTCCTCCCAGCTGCGCCACAGGCCGAACCACGGCAATCCCCGAA
ACGTGCGCCCTTGCCGCCGCAGTTTCCACGCACGGTGATTCGTCACGAACCCGAAAACACCCAGTGCGCCTGTGGCTGCCAACTTCAACGCATCGGTGAA
GACGTCAGCGAGAAGCTGGATTACACGCCGGGCGTGTTTACCGTCGAGCAACATGTGCGTGGTAAATGGGCCTGCCGTCAGTGCGAAACCCTGATTCAGG
CGCCGGTGCCAGCCCAGGTTATCGACAAAGGTATCCCGACCGCAGGTTTGTTGGCCCACGTGATGGTGGCCAAGTTTGCCGACTACTTGCCGCTGTACCG
GCAGGAGAAAATTTTTGGCCGCGCCGGGCTGGCGATTGCTCGCTCGACCTTGGCGCAGTGGGTTGGACAAACCGGCGTGCGACTCCAGCCGCTGGTCGAT
GCGTTGCATGAAGCTATGCTGAAACAGGGCGTGATCCACGCCGACGAGACACCGGTACAGATGCTTGCGCCAGGCGAGAAGAAAACCCACCGGGCCTATG
TCTGGGCCTACAGCACGACGCCGTTTTCGGCGCTCAAAGCGGTTGTTTACGACTTCAGCCCAAGCCGTGCTGGCGAACATGCACGCAACTTCCTGGGCGA
CTGGAACGGCAAGCTGGTCTGCGACGACTTCGCTGGCTACAAAGCCGGGTTTGAGCAAGGCATCACTGAAATCGGCTGCATGGCCCACGCCCGTCGCAAG
TTCTTTGATCTGCACGTGGCGAATAAAAGCCAGCTGGCTGAACAGGCCCTGCACTCGATCAGCGGCTTGTACGAGGTCGAACGCCAAGCGTGGGACATGA
GCGATGAAGACCGCTGGCGAATACGCCAGGAAATGGCGGTGCCGATCATCAAAACACTGCATGACTGGATGTTGGCCCAGCGAGACCTGGTGCCCAATGG
ATCAGCCACGGCCAAAGCCCTCGATTACAGCCTGAAACGCTGGGTAGCGCTGTCGCGCTACCTCGATGACGGGGCCGTACCCATAGACAACAACTGGGCA
GAGAACCAGATCCGGCCTTGGGCTCTTGGACGCAAGAACTGGCTCTTCGCAGGATCGCTACGCAGTGGAAAACGGGCGGCGGCGATCATGAGTTTGATCC
AGTCAGCACGGCTGAATGGGCATGATCCGTATGTTTACTTGAAGGACGTTCTCACGCGCCTGCCGACGCAGCGGGCTAGTGAAATTACAGAGCTACTGCC
GCACAAATGGATGCTAGCTTGAGCCGATATGAGGGCTGCAGACGTTGAACAGTGCACTTGAATCCTGAAAAACATGTCGGATATTCAGCAGCGTTTGCGA
GGGACAGTCAGTCGAAAAACTCCATCTCGCTATAAGGGTGACTTCGGCATGTAGCCAATCGTGATTTCAAATCACCAACATGAGCTTCCAGTTGATGGCC
ATCTGGGTCAAGGAAGTAGAGAGAGTCACCTTCACTCTTGTCTTGCCTCCAAAGCGGAACATTCAGTTCACGCAGACGTTGGGAGAATTCGGCGAAGTTT
TTCTGATCAATAGAAAAGGCATAGTGCGTGTAATTACGTTGCATGTCTTCTGCGCGCACACTATCACTAGAGAGACAGAGCCAGAGATCACCCAGGCTAA
GATACGCCCCAGTCTTCCAGCGCGCAGACAATTTGAAGCCCAGCGTTTCCTCATAAAAGGCAATGCTGCGGGGCAAGCTGCATACGGCGAGAGTGAGATG
ATTGAAGCCAGTCAGCATGAAGATCGCCTGAGACAGATGGACGGCTAGAATGTAGATCAGACAGGATGAAATGTCATCTTTAGCTGCAATGAAGCTCAGC
CCCTAGTGCTACACACCAGAATCATCAACCCAAGAGATCGTAAAGGTGGGGTGCCCGGACGGTTAC
Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
321 bp106 aa71391+No
ORF function : Accessory Gene
AG : IS66 TnpA

ORF sequence :

MRQRSSYPKPFKAQVVQECLQPGATVSSVAISHGINANVIRKWMPLYRDQPAATSLPAFVPLKAAPKRPVEMSVIIELPMAGQVITVKWPTSDPEGCAQF
IRAVAQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
390 bp129 aa334723+No
ORF function : Accessory Gene
AG : IS66 TnpB

ORF sequence :

MANLRSRGMRAIYPGCRSMIRIDAIWLATEPMDMRAGTETALARVIAVFGAAKPHCAYLFANRRANRMKVLVHDGVGVWLAARRLNQGKFHWPGIRNGCE
VELDNDQLQALVLGLPWQRVGAGGVISML

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1536 bp511 aa7872322+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTFSPNLDQMTPEQLRALAAQALQLQSQVEAMSRKIQNDQTVIEQLTYEIALLKRHKFAKRSEQISPAQSSLLDDLLDTDLEAIEAELKQLLPAAPQAEP
RQSPKRAPLPPQFPRTVIRHEPENTQCACGCQLQRIGEDVSEKLDYTPGVFTVEQHVRGKWACRQCETLIQAPVPAQVIDKGIPTAGLLAHVMVAKFADY
LPLYRQEKIFGRAGLAIARSTLAQWVGQTGVRLQPLVDALHEAMLKQGVIHADETPVQMLAPGEKKTHRAYVWAYSTTPFSALKAVVYDFSPSRAGEHAR
NFLGDWNGKLVCDDFAGYKAGFEQGITEIGCMAHARRKFFDLHVANKSQLAEQALHSISGLYEVERQAWDMSDEDRWRIRQEMAVPIIKTLHDWMLAQRD
LVPNGSATAKALDYSLKRWVALSRYLDDGAVPIDNNWAENQIRPWALGRKNWLFAGSLRSGKRAAAIMSLIQSARLNGHDPYVYLKDVLTRLPTQRASEI
TELLPHKWMLA

 

Blast result :
Comments
ISPa75 is 97% aa similar to ISPpu14.
There is an insertion of a copy of ISPa79 between the left end and the first ORF, in silico we have deleted it.
References
1] Lijun Zeng (2018) Direct submission.