ISBugl1
- Family IS3
- Group IS2
Isoform IS1417Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AB011023 | ND | Burkholderia glumae | Burkholderia glumae |
DNA section
IS Length : 1336 bp
Ends
IR Length : 29/40
IRL : TGGAGCAGCCCCCTGAAACACCGGACACAGTCACCCACTTACAATAACGG
IRR : TGGAGTTGCCCCCGTAACTCAGGACACGGCACCCACTTAGGTTGCTGAAT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGGAGCAGCCCCCTGAAACACCGGACACAGTCACCCACTTACAATAACGGGTAGGTATAAGACCGTGTTTTTGACTCACACCAGGCAGGAAGTGATGGAC
GTGTTGACGGGCCCGGAGCGTCGGCGGCGCTGGACGGCAGAGCAAAAGCTGGCGATGGTTCGCGAGAGTTTCGAGCCGGGGAAATCGGTTTCGATGGTAG
CGCGCCAGCACGGCGTGAATCCGAACCAGCTCTTTCACTGGCGCAAGCTGTACCAGGATGGAAGCCTGTCGGCGGTCAAGGCTGGCGAGGAAGTGGTTCC
GGCCTCGGAGTTGGCCGACGCGCTCAAACAGATTCGCGAGCTGCAGCGCATGCTCGGCAAGAAGACGATGGAGAACGAGATTCTTCGCGAGGCTGTCGAA
TACGGCCGGGCAAAAAAATGGATAGCGCACTCGCCATCGTTGCCGGAGGACAGCCAGTGAAACTGGTCTGCGAAGTCCTCGGCGTGTCGCGCTCGAACGT
ATCGGCACGCAGGTCGCGCGAGGCGACTTGGCGCGACGGGCGGCAGCCCAGATCGACCCACGATGCACCGGTGGTCGAGGCTATTGAACGGGTTATCAGC
GACTTGCCCAGCTACGGATATCGACGCGTATGGGGGACGTTGCGCCGCGAGCGTATCGCGGCGGGACAGGCACCGCTGAACGCCAAGCGCATTTACCGCG
TAATGCGCACGCATGGCCTGCTGATGCAACGCAGAGCGGCACCGGTTCGGCCGCAGCGCCGTCACGATGGCAAGGTTGCGGTGGAGCGCAGCAATCAGCG
CTGGTGTTCCGACGGCTTCGAGTTTCGCTGCGACAACGGCGAGCCGTTGCGCGTGACGTTCGCGCTCGACTGCTGCGACCGCGAAGCGATGAGCTGGGCG
GCCACGACGGCCGGCCATAGCGGCGACATCGTGCGCGACGTGATGCTGGCTGCGGTGGAAAGCCGGTTTGGGGATGTGCTGCATACCGAGTCCGAAATCG
AGTGGCTGAGCGACAACGGCTCGGGCTATACGGCCGAGGAGACGCGTCAGTTTGCGGCGCTGCTCGGCCTGAAGCCGTTGACCACGCCGGTGTGCAGCCC
GCAGAGCAACGGCATGGCGGAAAGCTTCGTGAAGACCATGAAGCGCGATTACGTCGCTATCATGCCGAAGCCGGACGCAGCGACCGCGGCCAGGAATCTG
GCCATCGCATTCGAGCACTACAACGAAAAGCATCCCCATAGCGCGTTGAAGTACCGCTCGCCTCGCGAGTTTCGACGTTCGACGGATTCAGCAACCTAAG
TGGGTGCCGTGTCCTGAGTTACGGGGGCAACTCCA
GTGTTGACGGGCCCGGAGCGTCGGCGGCGCTGGACGGCAGAGCAAAAGCTGGCGATGGTTCGCGAGAGTTTCGAGCCGGGGAAATCGGTTTCGATGGTAG
CGCGCCAGCACGGCGTGAATCCGAACCAGCTCTTTCACTGGCGCAAGCTGTACCAGGATGGAAGCCTGTCGGCGGTCAAGGCTGGCGAGGAAGTGGTTCC
GGCCTCGGAGTTGGCCGACGCGCTCAAACAGATTCGCGAGCTGCAGCGCATGCTCGGCAAGAAGACGATGGAGAACGAGATTCTTCGCGAGGCTGTCGAA
TACGGCCGGGCAAAAAAATGGATAGCGCACTCGCCATCGTTGCCGGAGGACAGCCAGTGAAACTGGTCTGCGAAGTCCTCGGCGTGTCGCGCTCGAACGT
ATCGGCACGCAGGTCGCGCGAGGCGACTTGGCGCGACGGGCGGCAGCCCAGATCGACCCACGATGCACCGGTGGTCGAGGCTATTGAACGGGTTATCAGC
GACTTGCCCAGCTACGGATATCGACGCGTATGGGGGACGTTGCGCCGCGAGCGTATCGCGGCGGGACAGGCACCGCTGAACGCCAAGCGCATTTACCGCG
TAATGCGCACGCATGGCCTGCTGATGCAACGCAGAGCGGCACCGGTTCGGCCGCAGCGCCGTCACGATGGCAAGGTTGCGGTGGAGCGCAGCAATCAGCG
CTGGTGTTCCGACGGCTTCGAGTTTCGCTGCGACAACGGCGAGCCGTTGCGCGTGACGTTCGCGCTCGACTGCTGCGACCGCGAAGCGATGAGCTGGGCG
GCCACGACGGCCGGCCATAGCGGCGACATCGTGCGCGACGTGATGCTGGCTGCGGTGGAAAGCCGGTTTGGGGATGTGCTGCATACCGAGTCCGAAATCG
AGTGGCTGAGCGACAACGGCTCGGGCTATACGGCCGAGGAGACGCGTCAGTTTGCGGCGCTGCTCGGCCTGAAGCCGTTGACCACGCCGGTGTGCAGCCC
GCAGAGCAACGGCATGGCGGAAAGCTTCGTGAAGACCATGAAGCGCGATTACGTCGCTATCATGCCGAAGCCGGACGCAGCGACCGCGGCCAGGAATCTG
GCCATCGCATTCGAGCACTACAACGAAAAGCATCCCCATAGCGCGTTGAAGTACCGCTCGCCTCGCGAGTTTCGACGTTCGACGGATTCAGCAACCTAAG
TGGGTGCCGTGTCCTGAGTTACGGGGGCAACTCCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 366 bp | 121 aa | 96 | 461 | + | No |
Description : First part of the transposase
ORF sequence :
MDVLTGPERRRRWTAEQKLAMVRESFEPGKSVSMVARQHGVNPNQLFHWRKLYQDGSLSAVKAGEEVVPDAASELADALKQIRELQRMLGKKTMENEILR
EAVEYGRAKKWIAHSPSLPEDSQ
EAVEYGRAKKWIAHSPSLPEDSQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1240 bp | 414 aa | 61 | 1300 | + | No |
Description : Second part of the transposase
ORF sequence :
DRVFDSHQAGSDGRVDGPGASAALDGRAKAGDGSREFRAGEIGFDGSAPARRESEPALSLAQAVPGWKPVGGQGWRGSGSGLGVGRRAQTDSRAAAHARQ
EDDGERDSSRGCRIRPGKKMDSALAIVAGGQPVKLVCEVLGVSRSNVSARRSREATWRDGRQPRSTHDAPVVEAIERVISDLPSYGYRRVWGTLRRERIA
AGQAPLNAKRIYRVMRTHGLLMQRRAAPVRPQRRHDGKVAVERSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWAATTAGHSGDIVRDVMLAAVE
SRFGDVLHTESEIEWLSDNGSGYTAEETRQFAALLGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAIMPKPDAATAARNLAIAFEHYNEKHPHSALKYRS
PREFRRSTDSATDA
EDDGERDSSRGCRIRPGKKMDSALAIVAGGQPVKLVCEVLGVSRSNVSARRSREATWRDGRQPRSTHDAPVVEAIERVISDLPSYGYRRVWGTLRRERIA
AGQAPLNAKRIYRVMRTHGLLMQRRAAPVRPQRRHDGKVAVERSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWAATTAGHSGDIVRDVMLAAVE
SRFGDVLHTESEIEWLSDNGSGYTAEETRQFAALLGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAIMPKPDAATAARNLAIAFEHYNEKHPHSALKYRS
PREFRRSTDSATDA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1205 bp | 405 aa | 96 | 1300 | + | Yes |
Chemistry : DDE
ORF sequence :
MDVLTGPERRRRWTAEQKLAMVRESFEPGKSVSMVARQHGVNPNQLFHWRKLYQDGSLSAVKAGEEVVPDAASELADALKQIRELQRMLGKKTMENEILR
EAVEYGRAKKMDSALAIVAGGQPVKLVCEVLGVSRSNVSARRSREATWRDGRQPRSTHDAPVVEAIERVISDLPSYGYRRVWGTLRRERIAAGQAPLNAK
RIYRVMRTHGLLMQRRAAPVRPQRRHDGKVAVERSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWAATTAGHSGDIVRDVMLAAVESRFGDVLHT
ESEIEWLSDNGSGYTAEETRQFAALLGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAIMPKPDAATAARNLAIAFEHYNEKHPHSALKYRSPREFRRSTD
SATDA
EAVEYGRAKKMDSALAIVAGGQPVKLVCEVLGVSRSNVSARRSREATWRDGRQPRSTHDAPVVEAIERVISDLPSYGYRRVWGTLRRERIAAGQAPLNAK
RIYRVMRTHGLLMQRRAAPVRPQRRHDGKVAVERSNQRWCSDGFEFRCDNGEPLRVTFALDCCDREAMSWAATTAGHSGDIVRDVMLAAVESRFGDVLHT
ESEIEWLSDNGSGYTAEETRQFAALLGLKPLTTPVCSPQSNGMAESFVKTMKRDYVAIMPKPDAATAARNLAIAFEHYNEKHPHSALKYRSPREFRRSTD
SATDA
Blast result :
Comments
ORFB as defined here does not exhibit a stop codon at the 5' end. By comparison to the equivalent stop codon carried by IS1417, this codon CGA codes for Arg. The entire reading frame continues upstream to codon 20. However, this sequence exhibit no initiation codon. Note the first T and the last A have been deleted compared to the sequence in the data base to conform to consensus IS3 family ends.
References
1] Takagi, M., and Yoneyama, K. (1998) Direct submission to Genbank.