ISBli34
- Family IS3
- Group IS150
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| CP014869 | ND | Brevibacterium linens | Brevibacterium linens Brevibacterium linens BS258 Brevibacterium epidermidis BS258 |
DNA section
IS Length : 1595 bp
Ends
IR Length : 48/56
IRL : ACTACCTGACGGCGGCCCAGATACCTGGCGTGGAGTGGTCCCCGAAAGTT
IRR : CTACCTGACGGGGGCGCAGCAACCTCGCGCTGGACTGGTCCCCAAAAGTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CCCCCAATTA | CACCAACAAC | 0 |
DNA sequence
CTACCTGACGGCGGCCCAGATACCTGGCGTGGAGTGGTCCCCGAAAGTTGGACTGTGATCAACACAAGCCAACTTGAAGGGACCATTCCATGCGTAAGGA
CTGTCTGATCACAGACGAACAAGCCAAAGCCGCGATCGAACTCTTCGACCAAGGGCACGGCTATGCCTCGACAGCCACGATCCTCAACGTCCACAAACCC
TCACTCAAGCCCCTCCACGACCGGTGGCTGGTGCGTGGAACAATATCGGTCATGGAACCGCACCAGCGCCGCCGGATACCGGCAGAGACGAAGATCGCGA
CTGCGAAACGGTATCTCGATGGCGAACACAAAGTCGTTTTAGCCAAGGAGCTTGGCCTGGCCTCCTCGCGCACCGTGGTTGATTGGGCGAAGAAATACCG
GGACAAGGGCGAAGACGCGTTCGCGACACCGCCGCCGGCCAAAGGCAAGACAGCACGGATGCGAGCCCTCGACAACGGTCAGGACCCGGATGCTGATCCG
GCCCCATCCAAGCCAGACCTGATGGCGAGGAAGACTCGTGTCGAGGACATCAGCCTGGCTGAGTATCGGCAGCTGCAAGACCAGCTGCTGCGGGCTGAAG
CGGAGAACGCGTACTTGAAAAAACTTCGGGCCTTGAGGCAAAAAGGGCAGCTGTGAAAGCACGCATCGTTGCCAGTCTCAAGGCGGACTATCCACTGCCA
TTGCTGCTGCAGGTCGCGGGTTTGGCCCGGTCGACGTTCTTCTACCACCAGAAACGCTTCGACGTGAGACCCGACCCATACGCGCAGTTGAGAACACTCA
TCAAGGACATCTTCGAGAAGAGTCACAAACGCTATGGGCACCGCAAGATCTGGGCTGTCTTGGTCAAACAGGGCATCGCTATAGCGAAGAAGACCGTGCT
GCGATTAATGCGTGACATGGGTCTGCAGACCGAGGTGCGGAAGAAGAAGTTCAACTCCCATCGCGGCACGGTCGGTGCCGTTGCTGACAACGTACTCAAT
CGGGAGTTCACCGCTGATGAACCGAATGAGAAATGGGTTTCTGACGTGACGGAATTCGCTGTTGAAGACAAGAAACTGTATCTGTCACCAGTGATCGATC
TGTTCGATGCCGGTGTGGTGTCGTACTCGATGTCGACGTCACCGAACACGGCATTGACGAACGAGTCGCTGACGCAAGCCTGTCAGGGACTGCGTCCCGG
GCAGACGCCGCTGGTGCATACCGATCAGGGGTTTCAGTATCAGCACGTGTCGTGGGCGTCGATCCTGGCCAAGCATGGTGCCACAGCGTCGATGTCGCGG
AAGGGAAACTGCTGGGATAACGCGATGGCGGAGAACTTCTTCGGGCAGTTGAAGTCCGAGATGTTCTATCTGCAGAAATTCGACACGGTCGAGGACCTCA
AGGCCGCGATCGATGAGTACATTCATTGGTATAACTACGAACGGATCTCGTCACGACTTGGCGGCCTTGCCCCGATGGAATATCGAAGCAAGACCGCCAA
GGATGGTGAGCCTATTTACGAACTATGCTGACCTCACAGCAGTCCAACTTTTGGGGACCAGTCCAGCGCGAGGTTGCTGCGCCCCCGTCAGGTAG
CTGTCTGATCACAGACGAACAAGCCAAAGCCGCGATCGAACTCTTCGACCAAGGGCACGGCTATGCCTCGACAGCCACGATCCTCAACGTCCACAAACCC
TCACTCAAGCCCCTCCACGACCGGTGGCTGGTGCGTGGAACAATATCGGTCATGGAACCGCACCAGCGCCGCCGGATACCGGCAGAGACGAAGATCGCGA
CTGCGAAACGGTATCTCGATGGCGAACACAAAGTCGTTTTAGCCAAGGAGCTTGGCCTGGCCTCCTCGCGCACCGTGGTTGATTGGGCGAAGAAATACCG
GGACAAGGGCGAAGACGCGTTCGCGACACCGCCGCCGGCCAAAGGCAAGACAGCACGGATGCGAGCCCTCGACAACGGTCAGGACCCGGATGCTGATCCG
GCCCCATCCAAGCCAGACCTGATGGCGAGGAAGACTCGTGTCGAGGACATCAGCCTGGCTGAGTATCGGCAGCTGCAAGACCAGCTGCTGCGGGCTGAAG
CGGAGAACGCGTACTTGAAAAAACTTCGGGCCTTGAGGCAAAAAGGGCAGCTGTGAAAGCACGCATCGTTGCCAGTCTCAAGGCGGACTATCCACTGCCA
TTGCTGCTGCAGGTCGCGGGTTTGGCCCGGTCGACGTTCTTCTACCACCAGAAACGCTTCGACGTGAGACCCGACCCATACGCGCAGTTGAGAACACTCA
TCAAGGACATCTTCGAGAAGAGTCACAAACGCTATGGGCACCGCAAGATCTGGGCTGTCTTGGTCAAACAGGGCATCGCTATAGCGAAGAAGACCGTGCT
GCGATTAATGCGTGACATGGGTCTGCAGACCGAGGTGCGGAAGAAGAAGTTCAACTCCCATCGCGGCACGGTCGGTGCCGTTGCTGACAACGTACTCAAT
CGGGAGTTCACCGCTGATGAACCGAATGAGAAATGGGTTTCTGACGTGACGGAATTCGCTGTTGAAGACAAGAAACTGTATCTGTCACCAGTGATCGATC
TGTTCGATGCCGGTGTGGTGTCGTACTCGATGTCGACGTCACCGAACACGGCATTGACGAACGAGTCGCTGACGCAAGCCTGTCAGGGACTGCGTCCCGG
GCAGACGCCGCTGGTGCATACCGATCAGGGGTTTCAGTATCAGCACGTGTCGTGGGCGTCGATCCTGGCCAAGCATGGTGCCACAGCGTCGATGTCGCGG
AAGGGAAACTGCTGGGATAACGCGATGGCGGAGAACTTCTTCGGGCAGTTGAAGTCCGAGATGTTCTATCTGCAGAAATTCGACACGGTCGAGGACCTCA
AGGCCGCGATCGATGAGTACATTCATTGGTATAACTACGAACGGATCTCGTCACGACTTGGCGGCCTTGCCCCGATGGAATATCGAAGCAAGACCGCCAA
GGATGGTGAGCCTATTTACGAACTATGCTGACCTCACAGCAGTCCAACTTTTGGGGACCAGTCCAGCGCGAGGTTGCTGCGCCCCCGTCAGGTAG
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 567 bp | 188 aa | 90 | 656 | + | No |
Description : First part of the transposase
ORF sequence :
MRKDCLITDEQAKAAIELFDQGHGYASTATILNVHKPSLKPLHDRWLVRGTISVMEPHQRRRIPAETKIATAKRYLDGEHKVVLAKELGLASSRTVVDWA
KKYRDKGEDAFATPPPAKGKTARMRALDNGQDPDADPAPSKPDLMARKTRVEDISLAEYRQLQDQLLRAEAENAYLKKLRALRQKGQL
KKYRDKGEDAFATPPPAKGKTARMRALDNGQDPDADPAPSKPDLMARKTRVEDISLAEYRQLQDQLLRAEAENAYLKKLRALRQKGQL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 993 bp | 310 aa | 539 | 1531 | + | No |
Description : Second part of the transposase
ORF sequence :
SGERVLEKTSGLEAKRAAVKARIVASLKADYPLPLLLQVAGLARSTFFYHQKRFDVRPDPYAQLRTLIKDIFEKSHKRYGHRKIWAVLVKQGIAIAKKTV
LRLMRDMGLQTEVRKKKFNSHRGTVGAVADNVLNREFTADEPNEKWVSDVTEFAVEDKKLYLSPVIDLFDAGVVSYSMSTSPNTALTNESLTQACQGLRP
GQTPLVHTDQGFQYQHVSWASILAKHGATASMSRKGNCWDNAMAENFFGQLKSEMFYLQKFDTVEDLKAAIDEYIHWYNYERISSRLGGLAPMEYRSKTA
KDGEPIYELC
LRLMRDMGLQTEVRKKKFNSHRGTVGAVADNVLNREFTADEPNEKWVSDVTEFAVEDKKLYLSPVIDLFDAGVVSYSMSTSPNTALTNESLTQACQGLRP
GQTPLVHTDQGFQYQHVSWASILAKHGATASMSRKGNCWDNAMAENFFGQLKSEMFYLQKFDTVEDLKAAIDEYIHWYNYERISSRLGGLAPMEYRSKTA
KDGEPIYELC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1442 bp | 480 aa | 90 | 1531 | + | Yes |
Chemistry : DDE
ORF sequence :
MRKDCLITDEQAKAAIELFDQGHGYASTATILNVHKPSLKPLHDRWLVRGTISVMEPHQRRRIPAETKIATAKRYLDGEHKVVLAKELGLASSRTVVDWA
KKYRDKGEDAFATPPPAKGKTARMRALDNGQDPDADPAPSKPDLMARKTRVEDISLAEYRQLQDQLLRAEAENAYLKKTSGLEAKRAAVKARIVASLKAD
YPLPLLLQVAGLARSTFFYHQKRFDVRPDPYAQLRTLIKDIFEKSHKRYGHRKIWAVLVKQGIAIAKKTVLRLMRDMGLQTEVRKKKFNSHRGTVGAVAD
NVLNREFTADEPNEKWVSDVTEFAVEDKKLYLSPVIDLFDAGVVSYSMSTSPNTALTNESLTQACQGLRPGQTPLVHTDQGFQYQHVSWASILAKHGATA
SMSRKGNCWDNAMAENFFGQLKSEMFYLQKFDTVEDLKAAIDEYIHWYNYERISSRLGGLAPMEYRSKTAKDGEPIYELC
KKYRDKGEDAFATPPPAKGKTARMRALDNGQDPDADPAPSKPDLMARKTRVEDISLAEYRQLQDQLLRAEAENAYLKKTSGLEAKRAAVKARIVASLKAD
YPLPLLLQVAGLARSTFFYHQKRFDVRPDPYAQLRTLIKDIFEKSHKRYGHRKIWAVLVKQGIAIAKKTVLRLMRDMGLQTEVRKKKFNSHRGTVGAVAD
NVLNREFTADEPNEKWVSDVTEFAVEDKKLYLSPVIDLFDAGVVSYSMSTSPNTALTNESLTQACQGLRPGQTPLVHTDQGFQYQHVSWASILAKHGATA
SMSRKGNCWDNAMAENFFGQLKSEMFYLQKFDTVEDLKAAIDEYIHWYNYERISSRLGGLAPMEYRSKTAKDGEPIYELC
Blast result :
Comments
ISBli34 is 63% aa similar to ISCod1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.
2] Zhu,Y. and Sun,C. (2016) Direct GenBank submission.
2] Zhu,Y. and Sun,C. (2016) Direct GenBank submission.