ISSpl1

  • Family IS3
  • Group IS150
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP025536 ND Streptococcus pluranimilium
Streptococcus pluranimilium TH11417
DNA section
IS Length : 1430 bp

Ends


IR Length : 20/25

IRL : TGAACTGCACCCCAAAAGTTAGACACAAAATCTAACGATTGGGGTGCTTT
IRR : TGTACTGACCCCAAAAAGTTGGACAACTCATTATTGAAAGGATTTAGTTC

Insertion site


Left flankDirect repeatRight flankDR Length
CTGGTTCAAATTCATGAACCAGT3
GTGTTAATGCATTATTGACACAA3
CTTGCCGCTTGGGCAAGTCTTTT3

DNA sequence

TGAACTGCACCCCAAAAGTTAGACACAAAATCTAACGATTGGGGTGCTTTTCCTATGAAGTTAAGTTATAAAGATAAACTAGAAATCTATGAGCTGAGAA
AGAACGGAATGTCGTGGCCTTGGATTAGCCAACAATATGATGTTAATATTTCTAATCTCACATACATGATGAACCTCATGGATCGGTATGGCGTAGAAGT
CGTTGAAAAAGGTAACAATCGGTATTATTCTCCCGAATTAAAGGAGGAAATAATAGATAAAGTCTTGATTCATGGTTGTTCTCAACTCTCAGTTTCTCTT
GATTATGCCTTGCCAAATCGAGGAATACTTCCAAATTGGATAGCGCAATACAAGAAAAACGGTTATACTATTCTTGAAAAACCAAGAGGGAGACCGAGCA
AGATGGGACGTAAACGCAATAAAACCTGGGATGAAATGACGGAATTAGAACGCCTTCAAGAGGAAAATGAACGTCTCCGAACTGAGGTGGCCTTTCTAAA
AAAGTTGAGAGAACTTCGTTTAAGGGACGAAGCTCTAGCGCGCGAACGGCAGAAACAATTAGAGACATGGTCCAAGAAGGATTCCGACTAGACCTCCTAC
TTGCGACAGCTAAAATGCCTCGCTCCACCTATTATTATCAAGTCAAGCAACTGGATAAACTCGACAAGAACAAAGCAATTAAGACTGAAATTCAAGCCAT
TTATGATGAACATAAAGGTAATTACGGCTATCGTCGAATTTATTTAGAACTCAGAAATCGTGGATTTGTGGTCAATCATAAGAAAGTTCAACGTCTCATG
ACAGTCATGGGCCTAGCAGCCCGCATTCGTCGTAAGCGCAAGTATTCTTCTTACAAAGGTGGAGTTGGTAAGAAGGCTGATAATCTAATTAAACGTCAAT
TCGAGGGCTCTAAGCCTTGCGAGAAGTGTTATACCGATGTGACGGAGTTTGCCTTACCTGAAGGAAAACTCTACTTATCACCTGTTCTTGACGGCTATAA
CAGCGAGATTATTGATTTCACTCTGTCTCGATCTCCTAACTTGAAACAGGTTCAGACCATGCTGGATAAGGCATTTCCAGCAGATTCATACAGTGGAACT
ATTCTTCATAGTGACCAGGGTTGGCAATACCAACACCAGTCCTACCATGATTTTTTAGAGTCTAAAGGCATTCTGCCATCTATGTCCCGCAAGGGAAATA
GTCCAGATAATGGCATGATGGAATCTTTCTTTGGCATTCTCAAATCTGAAATGTTTTATGGACTCGAGACATCTTATCAATCCCTTGACGAGCTTGAAGA
AGCTATTATAGACTATATTTATTACTACAACAACAAACGCATTAAAGTAAAACTAAAAGGACTTAGTCCTGTCCAATACAGAACTAAATCCTTTCAATAA
TGAGTTGTCCAACTTTTTGGGGTCAGTACA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
537 bp178 aa55591+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKLSYKDKLEIYELRKNGMSWPWISQQYDVNISNLTYMMNLMDRYGVEVVEKGNNRYYSPELKEEIIDKVLIHGCSQLSVSLDYALPNRGILPNWIAQYK
KNGYTILEKPRGRPSKMGRKRNKTWDEMTELERLQEENERLRTEVAFLKKLRELRLRDEALARERQKQLETWSKKDSD

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
915 bp304 aa4861400+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GGLSKKVERTSFKGRSSSARTAETIRDMVQEGFRLDLLLATAKMPRSTYYYQVKQLDKLDKNKAIKTEIQAIYDEHKGNYGYRRIYLELRNRGFVVNHKK
VQRLMTVMGLAARIRRKRKYSSYKGGVGKKADNLIKRQFEGSKPCEKCYTDVTEFALPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLDKAFPAD
SYSGTILHSDQGWQYQHQSYHDFLESKGILPSMSRKGNSPDNGMMESFFGILKSEMFYGLETSYQSLDELEEAIIDYIYYYNNKRIKVKLKGLSPVQYRT
KSFQ

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1346 bp448 aa551400+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKLSYKDKLEIYELRKNGMSWPWISQQYDVNISNLTYMMNLMDRYGVEVVEKGNNRYYSPELKEEIIDKVLIHGCSQLSVSLDYALPNRGILPNWIAQYK
KNGYTILEKPRGRPSKMGRKRNKTWDEMTELERLQEENERLRTEVAFLKKVERTSFKGRSSSARTAETIRDMVQEGFRLDLLLATAKMPRSTYYYQVKQL
DKLDKNKAIKTEIQAIYDEHKGNYGYRRIYLELRNRGFVVNHKKVQRLMTVMGLAARIRRKRKYSSYKGGVGKKADNLIKRQFEGSKPCEKCYTDVTEFA
LPEGKLYLSPVLDGYNSEIIDFTLSRSPNLKQVQTMLDKAFPADSYSGTILHSDQGWQYQHQSYHDFLESKGILPSMSRKGNSPDNGMMESFFGILKSEM
FYGLETSYQSLDELEEAIIDYIYYYNNKRIKVKLKGLSPVQYRTKSFQ

 

Blast result :
Comments
ISSpl1 is 88% aa similar to ISStin7.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISSpl1 was identified in Streptococcus pluranimilium TH11417 strain from cattle.
References
1] Yushan Pan (2017) Direct submission to ISfinder