ISBli10

  • Family IS3
  • Group IS3
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP017150 ND Brevibacterium linens
Brevibacterium linens SMQ-1335
Brevibacterium linens
Brevibacterium aurantiacum
DNA section
IS Length : 1528 bp

Ends


IR Length : 18/24

IRL : TGGAGCGGGACCACGGTGACAGAGCAAATCAGTCCGACCACGTAGGGCGA
IRR : TCAAGCGGTACCAGGGTGAGTGAGGCAGTGGTTAGGCTGCCTCTGTTTCG

Insertion site


Left flankDirect repeatRight flankDR Length
CCCTACGTGTCAGGCCTTGAAAAATTCCGCCT0

DNA sequence

TGGAGCGGGACCACGGTGACAGAGCAAATCAGTCCGACCACGTAGGGCGAGAACCAGGAAGCCAATCAATATGTCGAGTCTCGTTGTGGCCGAAGTAGGC
CAAAATGGGTCCATGAGTAGTTCCGGACCGCGTGCCGGTGGACCGTCCCGTCGAAGGTCGTTCACTCCCGCGCAGAAGCTTGAGTTGTTGACCCAGTACG
AGGACGCCTGCACCGGCAAGGAGGGCGGTGCTTTTCTGCGGGAACAGGGTTTGTATTCCTCGCAGATCGCTGAATGGCGAAAGCTTCGTGATGCCGGTGC
TCTCACCGGAAAGAAGCCCGGGGAAACCATTGGCAAGCTCTCTGCAGAACAGGTCGAGATCGCGCGTTTGCGCCGCCAGCTGGAGGTGAGTGAGGGCCGG
TTGGAACGAACCGAGGCTGCCTTGGGAATCATGGAAAAACTCTCGGCGTTCTTCGAGAACGCCATCAAGGAATCACCGGACGAGCCGAAGTCCAGAAAGA
AATGACCAGCGCCTACCGGCATTTGGTGGAGGCGGCTGTTCCGACGCGGCAGGCCGCGGCCCTGCTCGGCCTCTCACGCACCACGATCTACCGTCAACCT
GCCGCCCCGGTGGACCACGAGCCGGTGGTGCCGCCAAACAAGCTGTGTGCCGCGGAGCGGGCCGAGATCCTGGCGGCACTGAACTCCCCGGAGTTCGTGG
ACCTGGCGCCCCTGCAGGTCTACGCGAAGCTACTCGATGAGGGCATCTACCTGGGCTCGGTCTCCACGTTCTACCGGGTCCTGCAGGAGAACGAGCAGGT
CAAGGAGCGCCGCCGGCTGGCCAAGCACCCGGCCCGGGCCATCCCGGAGCTGGTCGCTACCGCACCTGGTCAGGTGCTCAGCTGGGATATTACGAAGCTG
GCTGGCCCGGTGAAGGGCAAGTACTTCGATTGTTACCTGATGGTGGACATCCATTCGAGGTTCATCGTCGGCGCGCACGTGCACGCCACCGAATCAGGGG
TCCTGGCGATGGAGATGATGAAGGAGATCTTCGGCATCCACGGCATCCCCGAAATCGTCCACGCCGACCGCGGGACCTCGATGACGTCGAAGACCGTTGC
CGGACTGCTCTCGGATCTGGAGGTCACCCGATCCCATTCCAGGCCCCGGGTGAGCAACGACAACCCGTACAGCGAAAGTCTCTTCAAAACCATGAAGTAC
GGGCCAACCTTCCCCGATCGCTTCGCGTCCCTGAGCGACGCGCGGGCCTTCATCAGTGAATTCGTGGATTGGTACAATCACGCTCATCAGCATTCTGGCA
TCGGGTTCCATACTCCAGCCAACGTCCACTATGGCTTCGCCGATAGCGTCGCCGTGAAACGCTCACAGACCTTGGCTGCCGCGCGGGCCAAACACCCCCA
AAGGTTCAGCAAGGCCACCGATCCCAAGATCCTGGCCCTACCCGGACCGGCATGGATCAACCAACCAAAGGAGAGCACCGAAACAGAGGCAGCCTAACCA
CTGCCTCACTCACCCTGGTACCGCTTGA
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
453 bp130 aa53505+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSSSGPRAGGPSRRRSFTPAQKLELLTQYEDACTGKEGGAFLREQGLYSSQIAEWRKLRDAGALTGKKPGETIGKLSAEQVEIARLRRQLEVSEGRLERT
EAALGIMEKLSAFFENAIKESPDEPKSRKK

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1104 bp367 aa3941497+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

GPVGTNRGCLGNHGKTLGVLRERHQGITGRAEVQKEMTSAYRHLVEAAVPTRQAAALLGLSRTTIYRQPAAPVDHEPVVPPNKLCAAERAEILAALNSPE
FVDLAPLQVYAKLLDEGIYLGSVSTFYRVLQENEQVKERRRLAKHPARAIPELVATAPGQVLSWDITKLAGPVKGKYFDCYLMVDIHSRFIVGAHVHATE
SGVLAMEMMKEIFGIHGIPEIVHADRGTSMTSKTVAGLLSDLEVTRSHSRPRVSNDNPYSESLFKTMKYGPTFPDRFASLSDARAFISEFVDWYNHAHQH
SGIGFHTPANVHYGFADSVAVKRSQTLAAARAKHPQRFSKATDPKILALPGPAWINQPKESTETEAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1445 bp461 aa531497+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSSSGPRAGGPSRRRSFTPAQKLELLTQYEDACTGKEGGAFLREQGLYSSQIAEWRKLRDAGALTGKKPGETIGKLSAEQVEIARLRRQLEVSEGRLERT
EAALGIMEKTLGVLRERHQGITGRAEVQKEMTSAYRHLVEAAVPTRQAAALLGLSRTTIYRQPAAPVDHEPVVPPNKLCAAERAEILAALNSPEFVDLAP
LQVYAKLLDEGIYLGSVSTFYRVLQENEQVKERRRLAKHPARAIPELVATAPGQVLSWDITKLAGPVKGKYFDCYLMVDIHSRFIVGAHVHATESGVLAM
EMMKEIFGIHGIPEIVHADRGTSMTSKTVAGLLSDLEVTRSHSRPRVSNDNPYSESLFKTMKYGPTFPDRFASLSDARAFISEFVDWYNHAHQHSGIGFH
TPANVHYGFADSVAVKRSQTLAAARAKHPQRFSKATDPKILALPGPAWINQPKESTETEAA

 

Blast result :
Comments
ISBli10 is 85% aa similar to ISAar46.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Sebastien Levesque (2017) Direct submission.
2] de Melo,A.G., Labrie,S.J., Dumaresq,J., Roberts,R.J., Tremblay,D.M. and Moineau,S. (2016) Direct GenBank submission.