ISPa77

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Pseudomonas aeruginosa
Pseudomonas aeruginosa
Pseudomonas aeruginosa ST233
DNA section
IS Length : 2597 bp

Ends


IR Length : 26/34

IRL : TGCGGATTCCACGGTCATCTGGCCACCCATTCCATGAGCATCTGACCACC
IRR : TGCGGATTCCACGCCATTCGGACACTCAGCCCACGCTGATCCGGACACCT

Insertion site


Left flankDirect repeatRight flankDR Length
TGAATTTCGACAGGCCGTAGGCGATGGCGATGGCCGACATCGCGACGCCC5

DNA sequence

TGCGGATTCCACGGTCATCTGGCCACCCATTCCATGAGCATCTGACCACCGATTCCACGGTGATCCGGCCACCCATTCCACGCGCATCCGGCCACTGATT
CCACGGCCATCCGGCCACTCAACCGGGCAGGCAGTAACGCAGGATTTCTTCACTACCATCGACCTCTTTTTCGAAGCAGAGAGGTCGTCGTGGAGCGTTT
ATCCATGCGTAAGATTCGCGAAGTACTACGTCTCAAGTTCGAGGTCGGACTATCGGCTCGCCAGATTGCGGTCAGCGTGCAGGTCGGTCGTGTCACCGTC
GGCGACTACCTCAATCGTTTTGCCGCCAGCGGTCTCAGTTGGCCCTGTTCGTTGTCCGATGCCGAGTTGGAGCAGCAACTGTTCCCGCCGGCCCCGGCGG
TTGCCAGTGAGAAGCGGCCTTTACCCGATTGGGCATGGGTGCATGCCGAACTGCGCCGCCCCGGGGTGACCCTGGCGCTGCTCTGGCAGGAGTACCGCCT
GAGCCAGCCTCAGGGCTTTCAGTACAGCTGGTTCTGTGAGCACTACCGAGCCTGGCAGGGCAAGTTGGACGTGGTGATGCGTCAGGAGCACCGCGTCGGC
GAGAAGCTGTTCGTCGACTATGCCGGCCAGACGGTGCCGGTTATCGATCGCCACAGCGGCGAGATCCGCCAGGCGCAGGTGTTCGTCGCGGTGCTCGGCG
CGTCCAGCTACACCTTCGCCGAAGCCACCTGGTCGCAGCAGCTGCCGGACTGGCTAGGCTCCCATGCCCGCTGCTTCGCCTTCCTCGGCGGCGTGCCGGA
GATCGTGGTGCCGGACAACCTGCGCAGCGCGGTGAGCAAGAGTCACCGCTACGAGCCGGACATCAACCCCAGCTACCGCGATCTGGCCGAGCACTATGGC
GTGGCGGTGGTGCCGGCGCGGGCACGCAAACCGCGCGACAAGGCCAAGGCCGAAGTCGGCGTGCAGGTGGTCGAGCGTTGGATCCTCGCCGCACTGAGGA
ATCGGCAGTTCTTCTCCCTGGATGAACTCAACACGGCCATCGCCGGGCTGCTGGAGCGGCTCAACCAACGCCCGTTCAAGAAGCTGCCGGGCTCCCGGCA
GTCGGCCTTCGACAGCCTGGATCGTCCGGCGCTGCGCCCCCTGCCGGAGCAACCCTACGTCTACGCCGAGTGGAAGAAGGCGCGGGTGCACATCGACTAC
CACGTCGAGGTCGATGGGCATTACTACTCGGTGCCGTATCAACTGGTGAAGAAGCAGCTGGAGGTGCGCCTGACGGCGCGCACCGTCGAGTTTTTCCACG
CCAACCAGCGAGTGGCCAGCCACCTGCGCTCAATGCACAAGGGCAGGCACAGCACGCAGGCCGAGCACATGCCCAAGAGCCATCGCGAGCATGCCGAGTG
GACGCCGCAACGGCTGATCCGCTGGGCCGAGCAGACCGGGCCGAACACCGCCGGCGTGATCCGGCACATCCTCGAACGGCGCATCCATCCGCAGCAGGGC
TACCGGGCCTGCCTGGGCATCCTGCGCCTGGGTAAAACCCACGGTGAGGCGCGTCTGGAGTTGGCCTGCCGTCGCGCCATCAGCCTCGGCACGTGCAGCT
ACAAGAGCCTCGAATCGATCCTGCGCCAGGGGCTGGAAAACCTGCCGCTAGCTCAAACCAACCTGCCGCTGCTGCCGGACGACCACGCCAACCTGCGCGG
ATCCGCCTACTACCACTGACCCCAAGGAATCCCACCATGCTGCCCCATCCGACCCTGGACAAGCTGCAAACCCTGCGCCTGCACGGCATGCTCAAGGCGC
TGAATGAACAACTGAAAACCCCGGACATCGACAGCCTGAGCTTCGAAGAACGCCTCGGCCTGCTGGTCGACCGCGAGCTGACCGAACGCGATGACAAGCG
CCTGAGCAGCCGCCTGCGCCAGGCCCGGCTCAAGCACAACGCCTGCCTCGAAGACATCGACTACCGCAGCCCGCGCGGACTGGATAAGGCGCTGATCTTG
CAACTGAGCAGTGGTCAGTGGCTGCGCGACGGCCTCAACCTGATCATCGGCGGCCCCACCGGTGTCGGTAAAACCTGGCTGGCCTGCGCCCTGGCCCACC
AGGCCTGCCGGGAGGGCTACAGCGTGCGCTACCTGCGCCTGCCACGTTTGCTGGAAGAACTGGGTCTGGCCCATGGCGACGGCCGCTTCGCCAAGCTGAT
GAGCAGCTACGCCAAGACCGACCTGCTGATCCTCGACGACTGGGGCCTGGCCCCGTTCACCGGCGAGCAACGGCGCGACATGCTGGAGCTACTGGACGAC
CGTTACGGCCAGCGCTCGACCATCGTCACCAGCCAGATGCCGGTGGACAACTGGCACGAACTGATCGGCGATCCGACCCTGGCCGATGCCATCCTCGACC
GCCTGGTGCACAACGCTTATCGGATCAATCTGAAGGGTGAATCAATGCGCAAACGGACGCAGAAATTGACGACGCCAGCCAACCCGGACTAACAATGCCA
CCCCTGCGTCGCTGCGCTCCGACTGCCTGTCCGAATGAGCGTGGAACAGGTGTCCGGATCAGCGTGGGCTGAGTGTCCGAATGGCGTGGAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1515 bp504 aa2051719+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKIREVLRLKFEVGLSARQIAVSVQVGRVTVGDYLNRFAASGLSWPCSLSDAELEQQLFPPAPAVASEKRPLPDWAWVHAELRRPGVTLALLWQEYRLS
QPQGFQYSWFCEHYRAWQGKLDVVMRQEHRVGEKLFVDYAGQTVPVIDRHSGEIRQAQVFVAVLGASSYTFAEATWSQQLPDWLGSHARCFAFLGGVPEI
VVPDNLRSAVSKSHRYEPDINPSYRDLAEHYGVAVVPARARKPRDKAKAEVGVQVVERWILAALRNRQFFSLDELNTAIAGLLERLNQRPFKKLPGSRQS
AFDSLDRPALRPLPEQPYVYAEWKKARVHIDYHVEVDGHYYSVPYQLVKKQLEVRLTARTVEFFHANQRVASHLRSMHKGRHSTQAEHMPKSHREHAEWT
PQRLIRWAEQTGPNTAGVIRHILERRIHPQQGYRACLGILRLGKTHGEARLELACRRAISLGTCSYKSLESILRQGLENLPLAQTNLPLLPDDHANLRGS
AYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17372492+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLPHPTLDKLQTLRLHGMLKALNEQLKTPDIDSLSFEERLGLLVDRELTERDDKRLSSRLRQARLKHNACLEDIDYRSPRGLDKALILQLSSGQWLRDGL
NLIIGGPTGVGKTWLACALAHQACREGYSVRYLRLPRLLEELGLAHGDGRFAKLMSSYAKTDLLILDDWGLAPFTGEQRRDMLELLDDRYGQRSTIVTSQ
MPVDNWHELIGDPTLADAILDRLVHNAYRINLKGESMRKRTQKLTTPANPD

 

Blast result :
Comments
ISPa77 is 99% aa similar to IS1491 (ORFA : transposase) and 97% aa similar to ISUnCu3 (ORFB : helper of transposition).
References
1] Benoit Valot (2017) Direct GenBank submission.