ISfinder Mirror Site

ISBmo1

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_JQKB01000021	ND	Belnapia moabensis	Belnapia moabensis DSM 16746

DNA section

IS Length : 4900 bp

Ends

IR Length : 19/24

IRL : CGGCATTCGGTAGGAGGCACACCTACCGGGCTCGTGAGGGCCGGATCATG
IRR : CGGCGTTAGGTAGCAACCACACCTTACCGAATAGCCGCTTTTGCCGCTCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GGACACCGCC	GACCTCTC	CGCCGTGGAA	8

DNA sequence

CGGCATTCGGTAGGAGGCACACCTACCGGGCTCGTGAGGGCCGGATCATGAGCGCCGCCCCTGTGCGAACTAGGATTGGGGCGGCATGGTGTCTGGAATG
GCGAGAAATAATTTGTTGGGTCCAGCAGGCGCGGTTTCCGACGTGTTCGGCGCCGGGCAAGCGATATGGGCGATCATTTGCACGGCTTGGGCCGTGCTCG
TCGCGGTGAGTGTGTGGTCGTTCCACACCATCCAGTCCTTCATGGATCAAGGATGGGGCGCGGTTTTCCTGGCTGGGCTTGGAGCCGCGACGGTCGCCAC
GTTTGCTGTTTCGACGCTATTCTTCGGTTTGGCTGGGTTGGCGATTGGCTATCGTCGTTTACGACCTCTGCCGACACCGCCGCAGCCGACGCCGCCTAAC
TTGGTCGGAGTGGCACCAATTCAGGCACCCGCCGTTGAGTTGCAGTCAGACGTCGCCGACCTACGGCAGCAAGTGGCAGCGTTTGAGCGATCAGCGCGGG
AGCGCTTCGAGCTTGGCCTCGCAGGTCTTGCCAAAGAGGTCGCGGAGGTAGCCGAACGCCTTGGAAAACTGGACCAACGAGCCGCCGGCCGTTTGGATGC
GCGTTACGACCATTTCCGGGATGCCATTGCCAAGATTGAGGCCGACGTTGCATCGCTATCCGCGCAGCAGGAAAGCTTGCGTGACAGCGTGGAGCGCCGG
TTTGGGGTCAGAGATGCCGAAGCATCGCTCGTGCTGTCGGACCGGACGATAACCCAACTGGCGGACAAGCTATTGAAGGCGAACCCATCCGACTACGCCG
ACGAGCAGGCATGGCGCAACGACTTTGCGGTCTGGCGGATGTATTTGATGGACTTTTGGGAAACCGCTGGCCGATGGGACATGCAATCCAACGACGCCGG
TGATCAAGCAGCGGATATGTTTCGGGTCGAAGACTTGGACTACCGTGCCGTTCAGGGGCAACCAGGATCAGCCATATCGAGTGATTTTGTGATGCAGAAC
AAATGGCGGTTGGTCGCCATCGTGACCGCCCGCCACAACGCGCAGTGCGATGCCGTCATGTGGCGTATTCATGCAGAGGCGCGGAGCAGGTCGATCCCAG
ACGGCGTTCCTTGGCCTCGATCTCGACCGTAACTTAACTCACTTTTACGTAATATAATCGTAATTTTCGCTCCGTTAAAAGGCGACTAGTGTAACTTTCT
GTTGCGGAGTGGTAGAAAACTGCCAAGCAGCAGATTGGCGTTTGGGGAGCAAAAGGGTGGCTTCACTCAAAGTGTTAGTCATATGCTGTTTTTTCATGAC
GGGATGCGGTTTCAGCGAATTTGCTGACAGAGCCACAAATCCAATAGTTTTGGATTTGATGAGAGATAAGGATTTATGGGCAACATATAACTACGAAACG
GTTAGTACTGACGCAGCCCGCCGTACTATATTCATACAATATCGGCGCGGTGGCACACGGGCGGATTTTGTGTGTGCGGAGCCGCCACCTGATGCCCTCC
AAGCTTACACAAATGCTCTTGCTGCGGCAGTGAAAGGTGGAGGAGCAGAGAGTGTCGCGCTAGAACTTAAGCGAACATTTGGCACTAACGCAGCTCCCAT
GCTTTACCGAAGTCAAGGTTTGCAGTTTTTACGAGATCAAGTATTCAATGCATGTTTGATGATGATGAACGGCGTTATTGATGCTCCTACCTACCGCGCT
TTACTACCCAAGTTGCTTGAGGCATCCGTTGATCTTATCAAAAACGAGCAACAGGCAGTTCAGATTGCAGCGGGAAGGCAACCTGCGGCTCTAACACCTG
GCAGTGCGCCAGCGACGGCTGGTGGTTCATCTACCGATGCCGGAAAATGAATGATACTTGTTAGCTGATATGCATTCTTGTGCGCCGATAGTTGCATTAC
TAGTCCAGCCTAATTTCCTTCGCCCCTATGGCCGCCATAACCTGCAACAGGAACACGGCGGTAAAGCCGCCCCTGGCGATCTTGTTGCGAATGTTCGGTT
CGGTGTCCCTCACGCCGATGGCCGCCAGCCGCTCCGCAAGCTGGGCGTAGGTGACGTTCCGGCGCTTCAGTTCGGCCTTGAGCAAGCCTTTGGCCCGCCC
CTGCCATTCAGCCGTGTTGGCCGTTTCCGCGTCACCCATGCTCGCACCCCTCACGATGGCGTATGCCATCATATCCGGTGCGATTGCCATTGTCAGTAGG
GTCTGATGCCATCATAATCGGTGCATAGGCATCGGATACGATGGCAATGTCCCAACACTTCCTCCTTTCCGCTAAGGCTCGCACGCTCTCCCTGGCGTCC
ATCCTCCGCATGAGCGACGACGAGGCCCATGCCGCCTTCGTGGCGATCCGTTGGTCTGCGAACGGTGGCGAGCCCGTGTGCCCGCGCTGCGGGTGCTTGG
CCATCTACACCTATGCCGCCCGCCGCATCTGGAAGTGCAAGGCGTGCAATCATCAGTTCTCGGTCACGTCCGGCACCATCTTCGCCAGCCGTAAGCTGCC
GATCCGGGACTATCTAGCGGCCATTGCCATCTTCGTGAATGCGGCTAAGGGACTGTCGGCGCTTCAGCTCGGCCGCGATCTGGACGTGCAGTACAAGACT
GCCTTCGTGCTGGCCCACAAGCTCCGCGAGGCCATCGGCTCCGGGCAGGCCGACACCACCCTGTCCGGGCATGTCGAGGTGGATGGCGCCTACTTCGGCG
GGACTGTCCGGCAGGAGAACCGCAAGGAAGATCGCAAGGACCGGCGCCTTGCCGTGAACCAGTCCGGCAAGCGGCAGGTGGTGGTGGTCATGCGGGAGCG
GGGCGGCCGGACGCTGCCCTTCGTATTCCGCTCAGAGGATGAGAGCGTTCCGACGATCCGCGCCCGCGTGGAAACTGGCACTACGATCTACGCCGACGAG
GCGCCCGGCTGGGATGCGCTGCACGCCCGCTACGATACCAAGCGCATCAACCACTCCATCGCCTTCGCGGACGATGGCGCCTGCACCAACCAAGCCGAGA
GCTATTTCTCCCGCCTGCGCCGCGCCGAGTGGGGCCAACACCACCACATCAGCGGCAAGTACCTTGGCGCCTACGCCCGTGAGATGGCTTGGCGGGAGGA
CACCCGGCGCCGTCCCAACGGGACGCTGCATGGGCTGGCAGCGGCGGCGGCGCTTGGGCATCCGGTGTCGCGGATATGGGCAGGCTATTGGCAGAGGGCA
GTCAATTAGTCTCAGAGGCCGCAAACGTCCTACTTGAGACCATTCTTGCCATTAAGAAGATTAGGAGCAAAAGCTTCCAGAGCTGCCTGTACAGCAGATG
CAGGAAGCTCTTTATCCTTTACCTTATTGCCAAGATAAATCGGCATCTGGTTTGGAATATAAGCTTCTCTGATCCATTTGCGAAATTCGCCTAACGCCTC
ATCCGGGTAGCAATATGGAGATTGGGGGTTTGAGGCAGACTGCGGGAAGTAGTTGGGGTAGTTGTGTTCATATTTGATGCGATTACCATACAAAACTTCA
AGGTTTTCTGAATTCCAGTATTTTGCCCAAGCTATTCCGACGCTAATGTCGGGTATAAACTTATTGCCCAGGCTGGCGCCCTGCCTCAAAAGCGTAACCA
TAAGGTCGGCTATCTCTTTAAATACGCTGAAGTAGCCCATGGGAACCGTATGATAGACTAGCGACACACGGTCGTGGAATTGCTGCCAGGCGATATCTGC
CGATCCTGACGGGTTCCAACCGACTTGTGAATATATGAATTCTTGAAAACCCTTTCGTGCAAGAGTTCTAAAACTCTTGGCGGCGTGGCCGTAATCACCT
TTTGCCTCAAAGGCGTAATATTCTAAAACTGCCATACAAACGGCGTCAGGAACCGCGTGATAAATTGTTCCGGCGCGTGGGATAGCAATAAATGCGATAG
AGTCATCCGCGCCGGCAGCCCGGATCAACTCCTTGATCTTCTGTTCGCGTGGCCTTGCTGGATACTCCTGCCAAGCGCTCGTGATGCGGATGATTTGCGC
GTGGTCTACTCCACACATACGAGCTAGGCCACGCAACGTCAGGAACGGAGCGCCATTGTTCAGCACCCCCATCCCCACACCATCTATTTCACGTTCAGCA
GCGATTTCGAGTTTGAGACCCAACTGCTTCGGGGCGGTGCTCAAACTAGCAGGTTTTGACGTTTTCATGAATGAAATCAGCCTCTTAGCCTGGTGCTCAA
GTGGCTATTTTCAGCACTTGGACCACCATTCCGACGAGCGCTGGTCTGGACATGTCTTTCGAGTTGTCACACAACACGATTGCACAAGAGACGGCCAGGT
AACGCTGGTCCTACCGGCGGCCTGACCATTCAGGCCGCCGGGACTCTGTCGTATAAGCGGTAGCGTGCTAAGAGTCACGCTGAGTCAGACAACCCCCACC
GCAGCAGCCGCCCGACTTTCTCCGCATTCCGCACGATCCCGCCCTTCCGCTGATGCCGCAGCGCCCGTGCCACGCCGTCCTCTACGGCTCTCCGAACCGC
TCCGCTCCCTGCGTCCATACCCCGCAGCACGATCACCCGGCTGGCAATTTCTGGGACGCTAAGCGGCTGATTGGCCTGTCGTAGCGCGTCCAGCACCGTT
CGGCCCATGCCAGCAAACTCCTCCGGTGCCAGCGTCCGCACATATTTCGTCTGGATCGACTCCACGGGATAGTTCGGATCGAGCTGCCGGATCACGGCAT
CCAGGCTCGCCAAGTCAGCATGGATCTGCCGCGACCGCGCCTGCGAGGCTTCCAACTCGCCCGCCAGCGTCGCCCGGCGTTTCACCAGGCCGGAGAGAAC
CAAGTCCGTCATGCGCTATTCCCTTTGGAAACAAAGGGATTTTACGCGGCAGAGCGGCAAAAGCGGCTATTCGGTAAGGTGTGGTTGCTACCTAACGCCG

Protein section

ORF number : 5

ORF 1

Length		Begin	End	Strand	Fusion ORF
540 bp	179 aa	593	1132	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MDARYDHFRDAIAKIEADVASLSAQQESLRDSVERRFGVRDAEASLVLSDRTITQLADKLLKANPSDYADEQAWRNDFAVWRMYLMDFWETAGRWDMQSN
DAGDQAADMFRVEDLDYRAVQGQPGSAISSDFVMQNKWRLVAIVTARHNAQCDAVMWRIHAEARSRSIPDGVPWPRSRP

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
240 bp	79 aa	2139	1900	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGDAETANTAEWQGRAKGLLKAELKRRNVTYAQLAERLAAIGVRDTEPNIRNKIARGGFTAVFLLQVMAAIGAKEIRLD

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
963 bp	320 aa	2247	3209	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSQHFLLSAKARTLSLASILRMSDDEAHAAFVAIRWSANGGEPVCPRCGCLAIYTYAARRIWKCKACNHQFSVTSGTIFASRKLPIRDYLAAIAIFVNAA
KGLSALQLGRDLDVQYKTAFVLAHKLREAIGSGQADTTLSGHVEVDGAYFGGTVRQENRKEDRKDRRLAVNQSGKRQVVVVMRERGGRTLPFVFRSEDES
VPTIRARVETGTTIYADEAPGWDALHARYDTKRINHSIAFADDGACTNQAESYFSRLRRAEWGQHHHISGKYLGAYAREMAWREDTRRRPNGTLHGLAAA
AALGHPVSRIWAGYWQRAVN

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
939 bp	312 aa	4168	3230	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MKTSKPASLSTAPKQLGLKLEIAAEREIDGVGMGVLNNGAPFLTLRGLARMCGVDHAQIIRITSAWQEYPARPREQKIKELIRAAGADDSIAFIAIPRAG
TIYHAVPDAVCMAVLEYYAFEAKGDYGHAAKSFRTLARKGFQEFIYSQVGWNPSGSADIAWQQFHDRVSLVYHTVPMGYFSVFKEIADLMVTLLRQGASL
GNKFIPDISVGIAWAKYWNSENLEVLYGNRIKYEHNYPNYFPQSASNPQSPYCYPDEALGEFRKWIREAYIPNQMPIYLGNKVKDKELPASAVQAALEAF
APNLLNGKNGLK

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
208 bp	145 aa	4168	4375	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MTDLVLSGLVKRRATLAGELEASQARSRQIHADLASLDAVIRQLDPNYPVESIQTKYVRTLAPEEFAGMGRTVLDALRQANQPLSVPEIASRVIVLRGMD
AGSGAVRRAVEDGVARALRHQRKGGIVRNAEKVGRLLRWGLSDSA

Blast result :

Comments

ISBmo1 is 80% (transposase) aa similar to ISBos1.

References

1] ISfinder annotation (2017)
2] Kyrpides,N., Huntemann,M., Han,J., Chen,A., Mavromatis,K., Markowitz,V., Palaniappan,K., Ivanova,N., Schaumberg,A., Pati,A., Liolios,K., Nordberg,H.P., Cantor,M.N., Hua,S.X. and Woyke,T. (2014) Direct GenBank submission.