ISBrsp1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF284858 | ND | Bradyrhizobium sp. | Bradyrhizobium sp. ORS285 |
DNA section
IS Length : 1218 bp
Ends
IR Length : 30/43
IRL : TGAGGCCGGGAAACTGGACCATTATTGGCTAGAGTCTTGCGCTGCCGATT
IRR : TGAGGCCGAGCTTTGGACCACCCAGAACTAGAGTTTTGCGCTCTGATACG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
TGAGGCCGGGAAACTGGACCATTATTGGCTAGAGTCTTGCGCTGCCGATTCCCCGGCTGGGAAGAGCGGAAGACGATGAAGGCCTCGAAGTTTTCGGACG
CCCAGAAGGCGTTCATTCTGAAGCAGGGCAAGGACGGCGTGCCGGTCACGGACATCTGCCGCAAGGCCGGGATCAGCCCAGCGACCTATTTTAGTTGGAA
GAAGAAGTACGACGGGCTGCTGCCGACCGAGATGCGGCGGCTGAAGCAGCTTGAGGACGAGAACGGCAAGCTGAGGAAGCTGGTGGCCGATCTGTCGCTC
GACAAGGAGATGCTGCAGGACATGATCCGCCGAAAGCTGTGAAGCCTGGTCGGAAGCGCAAGCTGGTCGACGAGATCTGCGGCAAGTGGCAGGTGTCGAT
CCGCAGGGCCTGCGAGGCCCTGGAGTTCGACAGGTCGACCTACCACTACAGATCCCGTCGCTCCGACCAGGCCGCCCTCGAACAACGGATCAGGGAGATC
TGTCAGGTTCGCGTCCGCTACGGTTATCGCCGCATTCACGTCGTGCTTCGTCGCGAAGGATGGCGCCACGGGCAGAACAAGACGCGGCGGGTCTATCCCG
AATTGGGCCTGCAATTACGCAATAAGGCGCCGAAGCGTCGAGTCAGGGCCAAGTTGCGGGATGATCGCAGGCCGGCGACGCGATCGAACGAGACCTGGGC
GATGGACTTCGTCCACGATCAGTTGGCAACCGGACGTAAGCTACGGGTGCTCACGATCGTCGACATCTTCTTCCGATTCTCGCCAGCGCTGACACCGCGG
TTCACTTTCCGCGGCACCGATGTCGTGGAGATACTGGAAGGGGTCCGCGATGAAGTGGGATTCCCGGCAACGATCCGTGTCGATCAAGGCACCGAGTTCG
TGTCGCGCGATCTCGACGTCTGGGCGTATCAGCGCGGCATCACGCTGGACTTCTCCAGGCCCGGCAAGCCGACGGACAATGCTTTCATCGAGGCGTTCAC
TTTCATCGAGGCGTTCAACGGACGCTTCCCGGGAGGAATGTCTCAATGCCCATTGGTTCCTGACGCTTGCTGATGCCGAGGAAAAAGTGGAGACTTGGCG
CAGGTACTACAACGAAGAACGGCCGCACGGCGCGATCGGCAACAAACCGCCGATTCTGCTGCAAAACCACGATGGCGCAACCAGCCCGCCACCGTATCAG
AGCGCAAAACTCTAGTTCTGGGTGGTCCAAAGCTCGGCCTCA
CCCAGAAGGCGTTCATTCTGAAGCAGGGCAAGGACGGCGTGCCGGTCACGGACATCTGCCGCAAGGCCGGGATCAGCCCAGCGACCTATTTTAGTTGGAA
GAAGAAGTACGACGGGCTGCTGCCGACCGAGATGCGGCGGCTGAAGCAGCTTGAGGACGAGAACGGCAAGCTGAGGAAGCTGGTGGCCGATCTGTCGCTC
GACAAGGAGATGCTGCAGGACATGATCCGCCGAAAGCTGTGAAGCCTGGTCGGAAGCGCAAGCTGGTCGACGAGATCTGCGGCAAGTGGCAGGTGTCGAT
CCGCAGGGCCTGCGAGGCCCTGGAGTTCGACAGGTCGACCTACCACTACAGATCCCGTCGCTCCGACCAGGCCGCCCTCGAACAACGGATCAGGGAGATC
TGTCAGGTTCGCGTCCGCTACGGTTATCGCCGCATTCACGTCGTGCTTCGTCGCGAAGGATGGCGCCACGGGCAGAACAAGACGCGGCGGGTCTATCCCG
AATTGGGCCTGCAATTACGCAATAAGGCGCCGAAGCGTCGAGTCAGGGCCAAGTTGCGGGATGATCGCAGGCCGGCGACGCGATCGAACGAGACCTGGGC
GATGGACTTCGTCCACGATCAGTTGGCAACCGGACGTAAGCTACGGGTGCTCACGATCGTCGACATCTTCTTCCGATTCTCGCCAGCGCTGACACCGCGG
TTCACTTTCCGCGGCACCGATGTCGTGGAGATACTGGAAGGGGTCCGCGATGAAGTGGGATTCCCGGCAACGATCCGTGTCGATCAAGGCACCGAGTTCG
TGTCGCGCGATCTCGACGTCTGGGCGTATCAGCGCGGCATCACGCTGGACTTCTCCAGGCCCGGCAAGCCGACGGACAATGCTTTCATCGAGGCGTTCAC
TTTCATCGAGGCGTTCAACGGACGCTTCCCGGGAGGAATGTCTCAATGCCCATTGGTTCCTGACGCTTGCTGATGCCGAGGAAAAAGTGGAGACTTGGCG
CAGGTACTACAACGAAGAACGGCCGCACGGCGCGATCGGCAACAAACCGCCGATTCTGCTGCAAAACCACGATGGCGCAACCAGCCCGCCACCGTATCAG
AGCGCAAAACTCTAGTTCTGGGTGGTCCAAAGCTCGGCCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 76 | 342 | + | No |
Description : First part of the transposase
ORF sequence :
MKASKFSDAQKAFILKQGKDGVPVTDICRKAGISPATYFSWKKKYDGLLPTEMRRLKQLEDENGKLRKLVADLSLDKEMLQDMIRRKL
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 819 bp | 272 aa | 255 | 1073 | + | No |
Description : Second part of the transposase
ORF sequence :
GRERQAEEAGGRSVARQGDAAGHDPPKAVKPGRKRKLVDEICGKWQVSIRRACEALEFDRSTYHYRSRRSDQAALEQRIREICQVRVRYGYRRIHVVLRR
EGWRHGQNKTRRVYPELGLQLRNKAPKRRVRAKLRDDRRPATRSNETWAMDFVHDQLATGRKLRVLTIVDIFFRFSPALTPRFTFRGTDVVEILEGVRDE
VGFPATIRVDQGTEFVSRDLDVWAYQRGITLDFSRPGKPTDNAFIEAFTFIEAFNGRFPGGMSQCPLVPDAC
EGWRHGQNKTRRVYPELGLQLRNKAPKRRVRAKLRDDRRPATRSNETWAMDFVHDQLATGRKLRVLTIVDIFFRFSPALTPRFTFRGTDVVEILEGVRDE
VGFPATIRVDQGTEFVSRDLDVWAYQRGITLDFSRPGKPTDNAFIEAFTFIEAFNGRFPGGMSQCPLVPDAC
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 998 bp | 332 aa | 76 | 1073 | + | Yes |
Chemistry : DDE
ORF sequence :
MKASKFSDAQKAFILKQGKDGVPVTDICRKAGISPATYFSWKKKYDGLLPTEMRRLKQLEDENGKLRKLVADLSLDKEMLQDMIRRKAVKPGRKRKLVDE
ICGKWQVSIRRACEALEFDRSTYHYRSRRSDQAALEQRIREICQVRVRYGYRRIHVVLRREGWRHGQNKTRRVYPELGLQLRNKAPKRRVRAKLRDDRRP
ATRSNETWAMDFVHDQLATGRKLRVLTIVDIFFRFSPALTPRFTFRGTDVVEILEGVRDEVGFPATIRVDQGTEFVSRDLDVWAYQRGITLDFSRPGKPT
DNAFIEAFTFIEAFNGRFPGGMSQCPLVPDAC
ICGKWQVSIRRACEALEFDRSTYHYRSRRSDQAALEQRIREICQVRVRYGYRRIHVVLRREGWRHGQNKTRRVYPELGLQLRNKAPKRRVRAKLRDDRRP
ATRSNETWAMDFVHDQLATGRKLRVLTIVDIFFRFSPALTPRFTFRGTDVVEILEGVRDEVGFPATIRVDQGTEFVSRDLDVWAYQRGITLDFSRPGKPT
DNAFIEAFTFIEAFNGRFPGGMSQCPLVPDAC
Blast result :
Comments
ISBrsp1 ORF1 is 81% aa similar to ISRso12 ORF1 and ORF2 is 63% aa similar to ISRso12 ORF2.
References
1] Chaintreuil,C., Boivin,C., Dreyfus,B. and Giraud,E. (2001) FEMS Microbiol. Lett. 194 (1), 83-86