ISAeca6

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Aeromonas caviae
Aeromonas caviae
DNA section
IS Length : 1198 bp

Ends


IR Length : 11/12

IRL : GGTAATCCCCCCGAAAAACTGAGGTGCTCATAAGTAGAATTTTCCCGTAC
IRR : GGTATTCCCCCCATTCTTAGGGGCACTCAAAAGTAGAGGTCATGCGGCCT

Insertion site


Left flankDirect repeatRight flankDR Length
GGTGGTAGTGTTATCATCAATAAT4

DNA sequence

GGTAATCCCCCCGAAAAACTGAGGTGCTCATAAGTAGAATTTTCCCGTACCTTGATACCCGGAGGTTCTACATGAAAACATCACGCTTCAGCGATGCCCA
GATCATCGCCATTCTTAACCAGGCCGAGGCTGGCTCCCCCGTTCCAGAGCTCTGCCGCGAACATGGCATCAGCTCCGCCACCTTCTACAAGTGGCGCTCT
AAATACGGCGGAATGGATGCCTCCCTCATGGCTCGCCTCAAAGAGCTGGAGGACGAGAACCGCCGCCTCAAGAAGATGTACGCCGAGGAGCGCCTCAAAG
CCGAGATCATCGCTGAGGCCATGGCAAAAAAGTGGTGACGCCATCTGCCCGCCGGGAGATGGCAACCAGGGCCGTAGCGGATTACGCCATCTCCATCCGC
CGGGCTTGCCAGGTGTTCAAGGTCAGTGAGGGCTGCTATCGCTATCAGCCGGTGCTGGCCGATGAAAATCAGGAGATTGCCGATTGGCTGCTGCGCCTCA
CCGCTAATCAGCGTAATTGGGGTTTTGGACTGTGCTTTCTGTACCTGCGTAACGTCAAAGGCTTTGGCTGGAACCACAAGCGGGTGTACCGGATTTATCG
CGCCCTTGAACTTAATCTGAGGATAAAGCCGCGAAAACGGCTGGTACGGGCCAAACCGGAGCCGCTGGCGGTCCCTGAAAAACCCAATCACTGCTGGTCG
ATGGACTTCATGCATGACCAGCTCGGTGATGGCCGTTCGGTGCGGCTGTTTAATGTGATCGACGACTTCAACCGCGAAGCACTGTGCATCGAAGTGGATT
TCTCCTTGCCTGCCACACGGGTCAAACGGGCGCTGGAGCAAGTGATCGCGTGGCGGGGAAAGCCGGTGGAGATCCGCTGCGATAATGGCCCGGAATATAT
CAGCGAGGAGCTCAAGCGCTGGGCTCAGCAGGAAGGGATAGCGCTACGGTATATCCAGCCGGGCAATCCCCAACAGAACGCGTATATCGAGCGGTTTAAC
CGCACGGTACGTTACGACTGGCTGGGTCATTACCTGTTTGAGTCACTGAATGAATTGCAGGCATTTGCCACAAACTGGTTGTGGACGTATAACCATGAGC
GGCCCAATATGGCGCTGGGTGGCTACACACCAAAACAGCGCCTAATGCAGGCCGCATGACCTCTACTTTTGAGTGCCCCTAAGAATGGGGGGAATACC
Recoding section
  • Recoding by frameshift
  • Frame -1
  • Type translational
  • Experimentally demonstrated No

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa72338+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

ATGAAAACATCACGCTTCAGCGATGCCCAGATCATCGCCATTCTTAACCAGGCCGAGGCTGGCTCCCCCGTTCCAGAGCTCTGCCGCGAACATGGCATCA
GCTCCGCCACCTTCTACAAGTGGCGCTCTAAATACGGCGGAATGGATGCCTCCCTCATGGCTCGCCTCAAAGAGCTGGAGGACGAGAACCGCCGCCTCAA
GAAGATGTACGCCGAGGAGCGCCTCAAAGCCGAGATCATCGCTGAGGCCATGGCAAAAAAGTGGTGA

 

Blast result :Comments : First part of the transposase
ORF 2
LengthBeginEndStrandFusion ORF
843 bp280 aa3171159+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

ATGGCAACCAGGGCCGTAGCGGATTACGCCATCTCCATCCGCCGGGCTTGCCAGGTGTTCAAGGTCAGTGAGGGCTGCTATCGCTATCAGCCGGTGCTGG
CCGATGAAAATCAGGAGATTGCCGATTGGCTGCTGCGCCTCACCGCTAATCAGCGTAATTGGGGTTTTGGACTGTGCTTTCTGTACCTGCGTAACGTCAA
AGGCTTTGGCTGGAACCACAAGCGGGTGTACCGGATTTATCGCGCCCTTGAACTTAATCTGAGGATAAAGCCGCGAAAACGGCTGGTACGGGCCAAACCG
GAGCCGCTGGCGGTCCCTGAAAAACCCAATCACTGCTGGTCGATGGACTTCATGCATGACCAGCTCGGTGATGGCCGTTCGGTGCGGCTGTTTAATGTGA
TCGACGACTTCAACCGCGAAGCACTGTGCATCGAAGTGGATTTCTCCTTGCCTGCCACACGGGTCAAACGGGCGCTGGAGCAAGTGATCGCGTGGCGGGG
AAAGCCGGTGGAGATCCGCTGCGATAATGGCCCGGAATATATCAGCGAGGAGCTCAAGCGCTGGGCTCAGCAGGAAGGGATAGCGCTACGGTATATCCAG
CCGGGCAATCCCCAACAGAACGCGTATATCGAGCGGTTTAACCGCACGGTACGTTACGACTGGCTGGGTCATTACCTGTTTGAGTCACTGAATGAATTGC
AGGCATTTGCCACAAACTGGTTGTGGACGTATAACCATGAGCGGCCCAATATGGCGCTGGGTGGCTACACACCAAAACAGCGCCTAATGCAGGCCGCATG
A

 

Blast result :Comments : Second part of the transposase
ORF 3
LengthBeginEndStrandFusion ORF
1088 bp362 aa721159+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKTSRFSDAQIIAILNQAEAGSPVPELCREHGISSATFYKWRSKYGGMDASLMARLKELEDENRRLKKMYAEERLKAEIIAEAMAKKVVTPSARREMATR
AVADYAISIRRACQVFKVSEGCYRYQPVLADENQEIADWLLRLTANQRNWGFGLCFLYLRNVKGFGWNHKRVYRIYRALELNLRIKPRKRLVRAKPEPLA
VPEKPNHCWSMDFMHDQLGDGRSVRLFNVIDDFNREALCIEVDFSLPATRVKRALEQVIAWRGKPVEIRCDNGPEYISEELKRWAQQEGIALRYIQPGNP
QQNAYIERFNRTVRYDWLGHYLFESLNELQAFATNWLWTYNHERPNMALGGYTPKQRLMQAA

 

Blast result :
Comments
ISAeca6 is 97% aa similar to ISAs22.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] F. Pfeiffer (2017) Direct submission
2] M. Blettinger, M.A. Zamora-Lagos, F. Pfeiffer, B. Habermann, S. Gruber (in preparation)