ISfinder Mirror Site

ISBth21

Family IS5
Group IS427

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
CP015351	ND	Bacillus thuringiensis	Bacillus thuringiensis MYBT18246 p150790

DNA section

IS Length : 843 bp

Ends

IR Length : 14

IRL : GATAGTGTCTACACAAAAAAATAACACCTGTTAAACTAGAATCAAAAACA
IRR : GATAGTGTCTACACGAGTTTCAGCCACTGAGAAATAGCGGCCAAGGTAAC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATTTTTTGAC	TA	GTCATAACAA	2

DNA sequence

GATAGTGTCTACACAAAAAAATAACACCTGTTAAACTAGAATCAAAAACATATTGGAGATGATTCGCTATGAGTAATCCATATAATAGACATGATGTAAC
AGATGAGAATTGGAATAAGTTGGAACCAATCATTACCGAGTTACTCGGGAAATGGGGCGGTTGTAACGCTAACGATAATCGTCTATTTGTGAATGCCTGT
TTATGGATCATTCGTACAGGTTCTCCATGGCGGGATCTACCAACTGAATATGGGAAATTTAACGCGGTACATCGTCGCTACAAGCGATGGTGTGATAAAG
GGATTTGGGATAAAATCCTAGCAAAGCTAATGGACGAGCCAGATTATGAATGGATAAGGATTGATGCTTCTCATTGTAAAGTGCATCCACATGCGGCAGG
AGCTGTCGGTGGAAATCAAGACATGGGACGCACAAAAGGGGGCTAAACACGAAGATTCATCTGGTTGTAGATGCCAATGGAAATCCTATAAAATTCACGA
TTACTGGTGGGGAAGTGTCCGATTTTACAGAAACAGTCGATTTGATTACAGGATTGCAGGCAGAATACCTTCTAGCTGACAAGGGCTATGATTCTGATGA
AACTGTTCGCTTTGCCAAAAACCAAGGAATGAATCCTGTGATTCCACCTAGAAAAAATAGAAACGAACAACGTGAATATGATAAACATCTTTACAAACTA
CGACATCTTGTTGAAAATGCCTTTTTGAAACTAAAAAGGTTTCGTGGCATCGCCACACGTTATACAAAAACCACTTCTGCTTTTAGAGGCGCCGTTACCT
TGGCCGCTATTTCTCAGTGGCTGAAACTCGTGTAGACACTATC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated No

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
378 bp	125 aa	69	446	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSNPYNRHDVTDENWNKLEPIITELLGKWGGCNANDNRLFVNACLWIIRTGSPWRDLPTEYGKFNAVHRRYKRWCDKGIWDKILAKLMDEPDYEWIRIDA
SHCKVHPHAAGAVGGNQDMGRTKGG

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
456 bp	151 aa	380	835	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SASTCGRSCRWKSRHGTHKRGLNTKIHLVVDANGNPIKFTITGGEVSDFTETVDLITGLQAEYLLADKGYDSDETVRFAKNQGMNPVIPPRKNRNEQREY
DKHLYKLRHLVENAFLKLKRFRGIATRYTKTTSAFRGAVTLAAISQWLKLV

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
767 bp	255 aa	69	835	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSNPYNRHDVTDENWNKLEPIITELLGKWGGCNANDNRLFVNACLWIIRTGSPWRDLPTEYGKFNAVHRRYKRWCDKGIWDKILAKLMDEPDYEWIRIDA
SHCKVHPHAAGAVGGNQDMGRTKKRGLNTKIHLVVDANNPIKFTITGGEVSDFTETVDLITGLQAEYLLADKGYDSDETVRFAKNQGMNPVIPPRKNRNE
QREYDKHLYKLRHLVENAFLKLKRFRGIATRYTKTTSAFRGAVTLAAISQWLKLV

Blast result :

Comments

ISBth21 is 74% aa similar to ISMasp6.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2017)
2] Hollensteiner,J., Poehlein,A., Sproeer,C., Bunk,B., Rosenstiel,P., Schulenburg,H. and Liesegang,H. (2016) Direct GenBank submission.