ISEae2
- Family IS3
- Group IS2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Enterobacter aerogenes | Enterobacter aerogenes |
DNA section
IS Length : 1332 bp
Ends
IR Length : 4/5
IRL : TGGGGCGGCCCCGAGAAACACCAGACAGTAGATGTATCTTAAAATAAGAG
IRR : TGTGGTTATAGGGGTCTGAAGCCCTGATCTTGATTAAGTTAACGATGCCC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TCAGGTTGCG | TACTTTATTT | 0 |
DNA sequence
TGGGGCGGCCCCGAGAAACACCAGACAGTAGATGTATCTTAAAATAAGAGATAGGCTTAAACATATGTCTAATACTGGTTCTAATTTTGAGATGACAGGA
ATACTGTTGGGGCAGGAGCTCCGCAAGCGTAAGACTCCGCAGGAAAAAATTGCCATTATCCGGCAGACCATGGAGCCGGGGATGACCGTCTCCCATGTTG
CTCGTTTGCATGGCATTCAGCCCAGCCAGCTATTTAAGTGGAAAAAACAGTATCTGGAAGGCAGCCTGACTGCCGTTGCTGCCGGAGAAGATGTCGTTCC
GGCATCAGAGCTGTCCGCCGCAAACAAGCAGATCCGTGAGCTTCAGCGCCTTCTGGGTAAGAAGACGATGGAAGTAGAGATCCTGAAAGAAGCCGTGGAG
TACGGACAGGCCCGAAAATGGATAGCGCATGCGCCCTTGTTGCCAAAGGACAAGGAATAGCCCAGGTCAGCAGCATTATGGGCGTGTCGCGTGCGCAATT
ATCACTGCGGGTTCACCGTTCTGCCGACTGGCAGGACGGGCGCACTCTCCGGCGAGATGAGGAAGCAGACGCAGAGGTCCTGTCGCGCATCCTCGGTATC
ATCAGCGATATGCCCAGCTATGGCTATCGCCGGGTGTGTGGAATAATGCGGAGACAGCTTCGCGATGAAGGTCTTCCCCCGGTGAATGCCAAACGGGTTT
ACCGTGTGATGAGCGAGCATAATCTGTTGCTGCTGCATGACAAACCACAACGGCCTCAGCGCGAGCATAACGGTAAAATCGCGGTAGCAGAGAGCAACCA
GCGCTGGTGCTCAGACGGCTTCGAGTTCGGATGTGACGACGGCGAAAAACTGCGCGTGACGTTCGCGCTGGACTGCTGCGACAGAGAGGCCATAGACTGT
GCCGCGAGCACCGGCGGCTATGACAGCATGACCGTACAGGACGTGATGCTGGGAGCCGTGGAGAAGCGCTTCGGAGCGATGCTCCCGGACAAGCCGGTGC
AGTGGCTGACAGACAACGGTTCGGCGTATACGGCGCATGACACGCGCAAGTTCGCCGCAGATCTGAATCTGGAAGTCTGCACAACGGCAGTGAGCAGTCC
ACAGAGTAACGGTATGGCCGAACGGTTCGTAAAGACGATGAAGGAAGACTACATCGCGTTCATGCCAAAACCGAATGTGCGAACGGCGTTGCAGAACCTT
GCTGAGGCGTTCAATCATTACAATGAAAACCATCCGCACAGCGCACTGGGATATCACTCCCCGAGAGAATACCGGCGCCTGCGGGCATCGTTAACTTAAT
CAAGATCAGGGCTTCAGACCCCTATAACCACA
ATACTGTTGGGGCAGGAGCTCCGCAAGCGTAAGACTCCGCAGGAAAAAATTGCCATTATCCGGCAGACCATGGAGCCGGGGATGACCGTCTCCCATGTTG
CTCGTTTGCATGGCATTCAGCCCAGCCAGCTATTTAAGTGGAAAAAACAGTATCTGGAAGGCAGCCTGACTGCCGTTGCTGCCGGAGAAGATGTCGTTCC
GGCATCAGAGCTGTCCGCCGCAAACAAGCAGATCCGTGAGCTTCAGCGCCTTCTGGGTAAGAAGACGATGGAAGTAGAGATCCTGAAAGAAGCCGTGGAG
TACGGACAGGCCCGAAAATGGATAGCGCATGCGCCCTTGTTGCCAAAGGACAAGGAATAGCCCAGGTCAGCAGCATTATGGGCGTGTCGCGTGCGCAATT
ATCACTGCGGGTTCACCGTTCTGCCGACTGGCAGGACGGGCGCACTCTCCGGCGAGATGAGGAAGCAGACGCAGAGGTCCTGTCGCGCATCCTCGGTATC
ATCAGCGATATGCCCAGCTATGGCTATCGCCGGGTGTGTGGAATAATGCGGAGACAGCTTCGCGATGAAGGTCTTCCCCCGGTGAATGCCAAACGGGTTT
ACCGTGTGATGAGCGAGCATAATCTGTTGCTGCTGCATGACAAACCACAACGGCCTCAGCGCGAGCATAACGGTAAAATCGCGGTAGCAGAGAGCAACCA
GCGCTGGTGCTCAGACGGCTTCGAGTTCGGATGTGACGACGGCGAAAAACTGCGCGTGACGTTCGCGCTGGACTGCTGCGACAGAGAGGCCATAGACTGT
GCCGCGAGCACCGGCGGCTATGACAGCATGACCGTACAGGACGTGATGCTGGGAGCCGTGGAGAAGCGCTTCGGAGCGATGCTCCCGGACAAGCCGGTGC
AGTGGCTGACAGACAACGGTTCGGCGTATACGGCGCATGACACGCGCAAGTTCGCCGCAGATCTGAATCTGGAAGTCTGCACAACGGCAGTGAGCAGTCC
ACAGAGTAACGGTATGGCCGAACGGTTCGTAAAGACGATGAAGGAAGACTACATCGCGTTCATGCCAAAACCGAATGTGCGAACGGCGTTGCAGAACCTT
GCTGAGGCGTTCAATCATTACAATGAAAACCATCCGCACAGCGCACTGGGATATCACTCCCCGAGAGAATACCGGCGCCTGCGGGCATCGTTAACTTAAT
CAAGATCAGGGCTTCAGACCCCTATAACCACA
Recoding section
- Recoding by frameshift
- Frame -1
- Type transcriptional
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 369 bp | 122 aa | 92 | 460 | + | No |
Description : First part of the transposase
ORF sequence :
MTGILLGQELRKRKTPQEKIAIIRQTMEPGMTVSHVARLHGIQPSQLFKWKKQYLEGSLTAVAAGEDVVPASELSAANKQIRELQRLLGKKTMEVEILKE
AVEYGQARKWIAHAPLLPKDKE
AVEYGQARKWIAHAPLLPKDKE
Blast result :Comments : 91% identical to ISECl1 Orf1ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 939 bp | 312 aa | 361 | 1299 | + | No |
Description : Second part of the transposase
ORF sequence :
EDDGSRDPERSRGVRTGPKMDSACALVAKGQGIAQVSSIMGVSRAQLSLRVHRSADWQDGRTLRRDEEADAEVLSRILGIISDMPSYGYRRVCGIMRRQL
RDEGLPPVNAKRVYRVMSEHNLLLLHDKPQRPQREHNGKIAVAESNQRWCSDGFEFGCDDGEKLRVTFALDCCDREAIDCAASTGGYDSMTVQDVMLGAV
EKRFGAMLPDKPVQWLTDNGSAYTAHDTRKFAADLNLEVCTTAVSSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALQNLAEAFNHYNENHPHSALGYHS
PREYRRLRASLT
RDEGLPPVNAKRVYRVMSEHNLLLLHDKPQRPQREHNGKIAVAESNQRWCSDGFEFGCDDGEKLRVTFALDCCDREAIDCAASTGGYDSMTVQDVMLGAV
EKRFGAMLPDKPVQWLTDNGSAYTAHDTRKFAADLNLEVCTTAVSSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALQNLAEAFNHYNENHPHSALGYHS
PREYRRLRASLT
Blast result :Comments : 83% identical to ISEcl1 Orf2ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1208 bp | 402 aa | 92 | 1299 | + | Yes |
Chemistry : DDE
ORF sequence :
MTGILLGQELRKRKTPQEKIAIIRQTMEPGMTVSHVARLHGIQPSQLFKWKKQYLEGSLTAVAAGEDVVPASELSAANKQIRELQRLLGKKTMEVEILKE
AVEYGQARKWIAHAPLLPKGQGIAQVSSIMGVSRAQLSLRVHRSADWQDGRTLRRDEEADAEVLSRILGIISDMPSYGYRRVCGIMRRQLRDEGLPPVNA
KRVYRVMSEHNLLLLHDKPQRPQREHNGKIAVAESNQRWCSDGFEFGCDDGEKLRVTFALDCCDREAIDCAASTGGYDSMTVQDVMLGAVEKRFGAMLPD
KPVQWLTDNGSAYTAHDTRKFAADLNLEVCTTAVSSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALQNLAEAFNHYNENHPHSALGYHSPREYRRLRAS
LT
AVEYGQARKWIAHAPLLPKGQGIAQVSSIMGVSRAQLSLRVHRSADWQDGRTLRRDEEADAEVLSRILGIISDMPSYGYRRVCGIMRRQLRDEGLPPVNA
KRVYRVMSEHNLLLLHDKPQRPQREHNGKIAVAESNQRWCSDGFEFGCDDGEKLRVTFALDCCDREAIDCAASTGGYDSMTVQDVMLGAVEKRFGAMLPD
KPVQWLTDNGSAYTAHDTRKFAADLNLEVCTTAVSSPQSNGMAERFVKTMKEDYIAFMPKPNVRTALQNLAEAFNHYNENHPHSALGYHSPREYRRLRAS
LT
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISEae2 is 92% aa similar to ISEcl1.
ISEae2 is 92% aa similar to ISEcl1.
References
1] Boyd D. (2017) Direct submission