ISfinder Mirror Site

ISAsh1

Family IS1595
Group ISSod11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
BCQV01000026	ND	Alicyclobacillus shizuokensis	Alicyclobacillus shizuokensis NBRC 103103 Alicyclobacillus macrosporangiidus CPP55

DNA section

IS Length : 1012 bp

Ends

IR Length : 17/23

IRL : CATGGCTATCCCCATGTCTCAGCTTGACTTGATACGCGAACGCATGTTTC
IRR : CATGGCTATCCCGTCAGTTCAGCAATGGTGATGGGACTGGCAGAAATGGA

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AAAGTCAAGA	AAATCAAA	TTATGAAATA	8
ACCGGGGATT	TACCACGA	AAACCTGGC	8

DNA sequence

CATGGCTATCCCCATGTCTCAGCTTGACTTGATACGCGAACGCATGTTTCCTAGAATCATTTTGAAAAACTAGAATCATGCATGACCGAGGCGACGCGCC
GATGTTTCAGCGCCCGATGACGCTTCTGGAGTTCCAAGCCAAATTCCCCACGGACGACGCATGCCGCAATTACCTGTTCTCCCTACGCTTTCCTGATGGA
TTCCGTTGCCCGCGGTGCGGGCATGACCGGTTCTACGTGATCCGGAGCCGACACCTGTTGCAGTGTCAGTCGTCCGCTTGCCGACATCAGACTTCCCTGA
CGGTCGGCACCATCTTCGAAAAGTCGCGCACCCCACTGCGCAAGTGGTTCTGGGCGATCTTCCTCGCATCCCACGACAAGCGGGGCATATCGGCGTTGAC
TCTTGCGCGCGAAATTGACGTCACCTACAAGACCGCTTGGGCGATGCTGCACAAGATCCGCCACGCCATGCGCACCCGCGAGCAATCCTACAAGCTCGGC
GGTATCGTCGAGGTGGACGAGACGTACGTCGGCGGTCCTGGCGAGAACCCGAAACCAGGGCGTGGTACCAACAAGACGCCCACCCTCGTCGCACTGTCGC
TGTCTCCCGACGGGAAGCCCGGCTACTTGCGCATGCAGGCCGTCGAGAATGTGAAGGGTGAGACCATCGCTGCCTTTCTGAATCAGCATGTCGAGAAGGA
AGCGACCATCGTGAGCGACGGGTTTCCCTCCTACGCGCCCGTGGCTCAAGACCGGCCGGTGTTGGCGATGAAATTCGACCCTAAGAATAATCCGGACCAT
ATGAAGTGGCTGCACCGCGCCGTGTCCAACGCCAAAACTTTTCTGCTGGGTACGTACCACGGGATCAAGGGCAAGCATCTGCAAGCCTATCTGAGTGAGT
ACACGTATCGGTATAACCGTCGCCATTTCCAGGGCGAATGGTTCAATCGCTTGCTACAGGCATCCATTTCTGCCAGTCCCATCACCATTGCTGAACTGAC
GGGATAGCCATG

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
906 bp	301 aa	102	1007	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFQRPMTLLEFQAKFPTDDACRNYLFSLRFPDGFRCPRCGHDRFYVIRSRHLLQCQSSACRHQTSLTVGTIFEKSRTPLRKWFWAIFLASHDKRGISALT
LAREIDVTYKTAWAMLHKIRHAMRTREQSYKLGGIVEVDETYVGGPGENPKPGRGTNKTPTLVALSLSPDGKPGYLRMQAVENVKGETIAAFLNQHVEKE
ATIVSDGFPSYAPVAQDRPVLAMKFDPKNNPDHMKWLHRAVSNAKTFLLGTYHGIKGKHLQAYLSEYTYRYNRRHFQGEWFNRLLQASISASPITIAELT
G

Blast result :

Comments

ISAsh1 is 71% aa similar to ISPaen6.

References

1] ISfinder annotation (2017)
2] Hosoyama,A., Noguchi,M., Numata,M., Tsuchikane,K., Hirakata,S., Uohara,A., Kitahashi,Y., Ohji,S., Ichikawa,N., Kimura,A., Yamazoe,A. and Fujita,N. (2016) Direct GenBank submission.