ISAlba3

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MEMJ01000011 ND Alphaproteobacteria bacterium
Alphaproteobacteria bacterium RIFCSPHIGHO2_12_42_13
DNA section
IS Length : 1120 bp

Ends


IR Length : 28/32

IRL : CCTGCGTATCCGGTTGTCACATAATGATGGTTGAGCTAAAGTGCATTGAG
IRR : CTTGCGTATCCGGTTGTCACATAATTGAGGTTTCGTCGTTGATTGTGGGG

Insertion site


Left flankDirect repeatRight flankDR Length
CGCGAACCAGGATTATTTGTTCATTGGA8

DNA sequence

CCTGCGTATCCGGTTGTCACATAATGATGGTTGAGCTAAAGTGCATTGAGGGGAAATGCGACGATGCCAAATGTAGCTCAAGATCAACAATCAGCGATCA
CAAGCGCGCCTCTTGAGTGCCCAATAAATCTTTTAGATTTCCAGAAGATGTTTCCAGATGAGGCCGCATGTCTTCGATATTTGGAACAGATGCGTTGGCC
AGGCGGTTTTGTCTGCGAGAAGTGCTCTCAATTAAGCGAGCCATTTAGAGTGAGCCTCCGCCCACGAGTGCTTAAATGCAGATCTTGCTATTATGAATCA
TCGGTTACTGCTGGTACAGTTATGCACCGCAGCAAAACCAGTATGCTTGTATGGTTTTGGGCTGCTTATCTTGTTTCAACGCAAACCCCTGGAATATCGG
CAATGGAGCTTCAGAAAAAATTGGGCATTGCTCGCTATGAAACGGCATTTCAGATTTTACACAAATTAAGAGGTGCGATGGTACGCCCATGCCGCGATAA
GATCGGCACTGAGTGGCCTATAGAATTGGACATCATTTTCATTGGAGGCAAGACGAGAAGCGGTATTCAGGGCAAAACGAATCAAGCGCCAGTAATCATT
GCAGTGGAAATTCGTCGTCGAGAAGTTCGCGATCCCAAGACCAGAAAGGTAGTTAAACGCGCTATTGCTGGCCGCCTAAGGGTGCAAAAACTACCGAATA
AAAAAGCTGTCTCTGTTGATCAATTTACAAGAAATTGCATCGTGCCTGGTGCTGCCATTATTAGTGATGATGGTACTGAATTCACAAACCTAAGCAATTT
GGGCTTTGACCATCAACCAGTTCCAATGCGCGGAGACAGAGTCAAAATGGATAGCACATTGCCCATGGTCAGCTTAGTCACAGCCAATTTGAAAACATGG
ATTGACGGCACATTCCATGGTGTTCGAAAAAAACATTTACAAGCCTATCTGGATGAGTTCATGTTTAGATTCAATCGTCGTTTCTATCGTTCGGTATCAT
TCCGTTCTTTGCTAGGCATAGGAACGCTTAATACGGGCATTACCTACAAAGAGGTCTATGCTGTTTTGGACCCCACAATCAACGACGAAACCTCAATTAT
GTGACAACCGGATACGCAAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1041 bp346 aa641104+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPNVAQDQQSAITSAPLECPINLLDFQKMFPDEAACLRYLEQMRWPGGFVCEKCSQLSEPFRVSLRPRVLKCRSCYYESSVTAGTVMHRSKTSMLVWFWA
AYLVSTQTPGISAMELQKKLGIARYETAFQILHKLRGAMVRPCRDKIGTEWPIELDIIFIGGKTRSGIQGKTNQAPVIIAVEIRRREVRDPKTRKVVKRA
IAGRLRVQKLPNKKAVSVDQFTRNCIVPGAAIISDDGTEFTNLSNLGFDHQPVPMRGDRVKMDSTLPMVSLVTANLKTWIDGTFHGVRKKHLQAYLDEFM
FRFNRRFYRSVSFRSLLGIGTLNTGITYKEVYAVLDPTINDETSIM

 

Blast result :
Comments
ISAlba3 is 66% aa similar to ISCARN100.
References
1] ISfinder annotation (2017)
2] Anantharaman,K., Brown,C.T., Hug,L.A., Sharon,I., Castelle,C.J., Probst,A.J., Thomas,B.C., Singh,A., Wilkins,M.J., Karaoz,U., Brodie,E.L., Williams,K.H., Hubbard,S.S. and Banfield,J.F. (2016) Nat Commun 7, 13219.