ISAzbe2

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
FOFJ01000022 ND Azotobacter beijerinckii
Azotobacter beijerinckii DSM 378
DNA section
IS Length : 1041 bp

Ends


IR Length : 24/26

IRL : TGGGCTATCCACTTAGCTCCTTGGACGGCAAACTGCTCGTATGAACGAGC
IRR : TGCGGGCTATCCACTTAGCTCCTTGGTGGACTTTTCGGCGGATTGACAAG

Insertion site


Left flankDirect repeatRight flankDR Length
TAGGCCTGTATCGGGAAACAGGTGTTTG8

DNA sequence

TGGGCTATCCACTTAGCTCCTTGGACGGCAAACTGCTCGTATGAACGAGCACCGAGCACCTCCCGTTGCAGGGCACGACTATCCCCAAACATGGAGCCAA
TTCACGGATTGGTTTCATAACGAAGGTCCCTGCCTCGACTATCTGGAGCGGCTACGCTGGCCGCAACAGTTCATTTGTCCGAAGTTCGGAATCATTGGCG
CACCTCTCAAAACAAGTCGTAACCGACTGGTTTGCCAGGCATGCAGTCACCAAACCAGCGTGACCGCAGGAACGATTTTCGACAAAACGAGAACCCCGCT
CAAGGTATGGTTGGCCGCCGCGTGGTACATCACCAATCAGAAACATGGCGTCAGCGCCTTGGGGTTGCAGCGCGTTCTGGGACTTGGAAGCTATCAGACA
GCGTGGACCCTGCTTCATCGCTTTCGTCGTGCGATGATTCGCCCTGGCAGAGAGCTGCTCAGCGGTCTTGTAGAAGTCGATGAAACTTACGTTGCCATTG
GCGATAAATCGCAACCGGTTGATGTGAAAGGCCGTAAGTCCCGCACGGCCAAGACCTTGGTCATCATTGCTGTAGAGATGTTGGAGCCCAAAGGGTTCGG
GCGAATACGCCTGCGCCGAATACTTGATGACTCTGAACACCAGGTCATCCCTTTTGTGAAGGATGTCGTTCAAGTGGGGGCGACAGTGCATACCGATGGA
TCGGCTGCTTACCGAAGCCTTGAGCGCGAGGGATATGTTCACCGAAAAAGTGTCATGCTCGGCTCGCCAACAGCAGCACATGCCTCAATGCCAGGTGTTC
ACAGGGTAGCCGCGCTCATCCATCGATGGTTACTTGGAACACACCAAGGCGCAGTGCAACCTTCCAAGCTCGACAGTTACCTTGACGAGTTCGTTTTTCG
GTTCAACAGGCGAAAATCGTCGTCACGCGGCATGCTGTTCTACAGGTTGCTGGAGCAGGCTGTTGTCACCACACCCATGACGTACCAGAACCTTGTCAAT
CCGCCGAAAAGTCCACCAAGGAGCTAAGTGGATAGCCCGCA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
987 bp328 aa411027+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNEHRAPPVAGHDYPQTWSQFTDWFHNEGPCLDYLERLRWPQQFICPKFGIIGAPLKTSRNRLVCQACSHQTSVTAGTIFDKTRTPLKVWLAAAWYITNQ
KHGVSALGLQRVLGLGSYQTAWTLLHRFRRAMIRPGRELLSGLVEVDETYVAIGDKSQPVDVKGRKSRTAKTLVIIAVEMLEPKGFGRIRLRRILDDSEH
QVIPFVKDVVQVGATVHTDGSAAYRSLEREGYVHRKSVMLGSPTAAHASMPGVHRVAALIHRWLLGTHQGAVQPSKLDSYLDEFVFRFNRRKSSSRGMLF
YRLLEQAVVTTPMTYQNLVNPPKSPPRS

 

Blast result :
Comments
ISAzbe2 is 82% aa similar to ISAisp8.
References
1] ISfinder annotation (2017)
2] Varghese,N. and Submissions,S. (2016) Direct GenBank submission.