ISfinder Mirror Site

ISAcba1

Family IS1595
Group ISSod11

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
MKSW01000042	ND	Actinobacteria bacterium	Actinobacteria bacterium 69-20

DNA section

IS Length : 3517 bp

Ends

IR Length : 24/27

IRL : ATTGGGTATCCGCTTGAGTCCAGTAGGTTTGAGTGGTGATCGAGCGGCTG
IRR : AGTGGGTATCTGCTTGAGTCCAGTCGGCCGATCTTCGACGGAACTACCTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GAGCACTCGT	ACAGTTGT	TCGATGGTCT	8

DNA sequence

ATTGGGTATCCGCTTGAGTCCAGTAGGTTTGAGTGGTGATCGAGCGGCTGCCGGTGGCCGGCGTGGATTACCCGCGCACGCATCAGCAGCTGTCCGCGTG
GTTCTCGGACGAGGACGCGTGCCTGGAGTACCTGGCCCGCTTGCGCTGGCCAGACGGATTCCGCTGCCCCTCGTGCGGTGGGGATGCCGCCTGGCGCACC
GCGCGGCGCGCGTTGTGGATGTGCGCAGGGTGCGGGCGGCAGACGTCGGTCACGGCCGGCACGATCTTCCACCGCTCGCACACCCCGTTGACGACGTGGT
TCGCGGCGATCTGGTTCGTGTGCGCGTCGAAGAACGGGGTCTCGGCGAGCACGTTGCAGCAGGTCCTCGGGTTCGGCTCGTACGAGACCGCGTGGGCGTG
GCTGCACAAGCTACGCCGGGTCATGGTTCGCCCCGATCGTGACCTGCTCGGCGGGGTCGTCGAGCTGGACGAGACCCTCGTCGGCGGGCGCGCCAAGCCC
GCCTCCAGCGTGGTGATCACCAAGGTCCCGGTCATGGTCGCGGTCGAGACCCCGGCCGGGCACCAGCTCGGACGCATTCGCCTGGCCCGCGCCGACGCCC
GTAACTCGGGCCAGCTGATCGACTTCGCCCACCAGGTCATCGCCCCTGGCACGTTGATTCGCACCGACGGTGCCCGCAACCTGCGCCGGCTCGCCGAGCA
GGGCTACACCCACGAGTACAAGGTCATGCTGACCTCACCGGACCCGGCCCATGAGTACCTGCCTGGCGTGCACCTGGTCGCTTCCCTGCTCAAACGCTGG
GTCGCCGGCACGCTGCACCACGGCATCTCCCGCCGCCAGCTCGACTACTACCTCGACGAGTTCACCTTCCGGTTCAACCGGCGCAGCTCACGCTCCCGCG
GCCTGCTGTTCTACCGGCTCCTGCAACAAGCCGTCGCCACCGACCCGCACCCATTGGCCGCACTGGTCGGCGGCACCGACCAAGACCGAGGTGGCGGCGC
GTCGTAGCAGCGTCTATCGGGCCATGGCCCGCTGTGGACTCTGCACGCTGGTCACGGTGCTTGTGGTGTGCAGGTTCGTGGCCCGTCCGGCGCGCACGCC
GTTGGCGATGACGACGACCTCGGCCAGCTCATGGACCAGGACGACCGCGGCCAGCCCAAGCACTCCGGCGACTGCCAGCGGGATCAGGATCGCGATCAGG
GTGAGCGACAGGCCGACGTTCTGCAGCATGATGCGCCGGGCCCGTCGGGCGTGGGCGAATGCCTGAGGCAGGGCGCGCAGGTCCGTGCCCATCAGGGCGA
CGTCCGCGGTCTCCAGTGCGACGTCCGAGCCCATGGCGCCCATCGCGATGCCGAGGTCGGCGGTGGCCAGGGCGGGGGCGTCGTTGACGCCGTCGCCGAC
CATTGCTGTGGGTGCCTTCGTACGCAGGGCGGCGACCAGCGTGGACTTGTCCTCTGGACGCAGCTCGGCGTGGACGTCGGCGATGCCGGCCTGGGCGGCC
ACGGCGTAAGCGGTGGCGGTGTTGTCGCCGGTAAGCATGGTGACGTTGTAGTCGTGGCGGTGCAGCTCGGCGACGACCGCGCGCGCTTCGGGTCGCAGCT
CGTCTCGGACGGCGATCGCGCCGGTCACCGTCCCGTCGTCCTCGACCAGGACGGCCGTCGCCCCGGCTTCCTGCATTCCCTGGACCTGGGCGGCCAGGTC
CGCGGCGGTGATCCACCCGGGGCGGCCCAGCCGCACGGCCCGCCCGTCGACGGTGCCGGTCAGGCCGGCGCCGGGGACCGCCTGGACATCAGCGGCGTCG
GCCACGGTGTCGACCGCGGTGAGGATCGCCCGGGCCAACGGGTGCTCGCTGCGGGCCTCCAAGGCGCCCGCCACGGCGAGGACCTGCTCGCGGGTGTGGC
CCGCGGTGGTGGCGACGGCGACGACGGTGGGGGCGTTGGCTGTCAGGGTGCCGGTCTTGTCCAGGGCGATCATGCGGATCGCGCCGAGCGCCTCCAGGGC
GGCGCCACCCTTGATCAGCACGCCGAATCGGCTGGCGGCCCCAATGGCGGAGACGACCGAGACCGGCACGGAGATGGCCAACGCGCACGGGGACGCGGCT
ACCAGCACGACCAGCGCCCGTTGGATCCATAGTCCCGGGTCCCCGAGCAGGGAGCCGATCACTGCGATGAGGGTGGCGACGATCATGATGGCGGGCACGA
GCGGCTTGGCGACCCGGTCGGCGAGCCGCTGGGTGGTGCCCTTGCGCGACTGCTCGGCCTCGACGATGTGCACGATGCGGGCCAGGGAGTTGTCTTCCGA
AGTAGTGGTGACCTCGACCTCGAGGGCTCCGGTGCCGTTGATGGATCCCGCGTACACCTGGGTGCCGGGTCCGACCTCGACGGGGATCGATTCTCCGGTG
ATGGCCGAGACGTCGAGGGTGGTGCGGCCGGTGCGGATGGTGCCGTCGGTGGCGAGCCGTTCCCCCGGCTTGACGAGCATCGTGTCGCCCACAGCGAGAT
CGTCCGGGTCGATGACCTGCTCGATTCCCGCGCGTCGGACCGTGGCCGTGTCGGGGACCAGGGAGAGGAGGGCCCTCAGCCCACCGCGGGTCTTGGCCAG
GGAGTACTCCTCCAGGCCCTCGCTCAGGGAGTACAAGAACGCGAGGGTGGCGGCCTCGGCGAACTCACCGAGGATCACTGCGCCGACGGCGGCGATCGTC
ATCAAGGTGCCGACGCCGATCTTGCCCCGGGCCAGCTTCTGCAGGGTCTCGGGCACGAAGGTGCTCGCCCCGACCAGCAGGGCGGCGATGTTCAAGCCGA
GCGCCCAACCGTGCAGGCCGCCCAGTCCGAGCAGGAAGGCAACCAGCAGGAGCGTGCCCGCGACGAGACCGGCGCGGATCTCGCTGACACCCCAGATGCT
CGTGACGTCCTTATCGTCGTCATCGTCGATCTCATCGGCGGTGGTCTGATCGGCGCCGCGGTCGTCGGTCATCGGGTGCTCTCCTCGTGGGTTGATGTGT
TCGCGGGCATCGAGGTCTGGCTGCCCGTCCGGCCAAGTCCGTAAGTGGGGCACAGCTCGACCGCGTGCCCGGTCGCGTCCAGCAACAGCTCGGCCGCGGC
GAGGACCGCAAGCAGCTCCGGGGCCGTTGTCAGGAAGAACATCGACGCCCGACCCTGCGGGCGGGAGTCGACCAGGCCACAGTCCTTCAGGCACGCCAGG
TGTGCGCTCACCGTGCTCTGCGCCAGTCCGGTGTGCTCGGTGAGATCCCGCACTCGATGCTCACCGTGCGTCAGATGCTGCAGGACGGCCAGGCGCGTCG
GGTCGGAGAGCGAGTGGAACAGCGCCGCCGCCGGGGCCAGCACACCGCCCGCGGGATCGCCGGGTTCCCCGTCGACAACCCGATCCGGTCGTATCGCCAT
GTGACGATGATAGCGGCAGGTCTGGATGGGTCCTGCCTGGCTCGCGCGCCGCCGGAGCACAAGACACGAGGTAGTTCCGTCGAAGATCGGCCGACTGGAC
TCAAGCAGATACCCACT

Protein section

ORF number : 3

ORF 3

Length		Begin	End	Strand	Fusion ORF
432 bp	143 aa	3400	2969	-	No

ORF function : Passenger Gene
Annotation : Transcriptional regulatorDescription : Transcriptional Regulator factor

ORF sequence :

MAIRPDRVVDGEPGDPAGGVLAPAAALFHSLSDPTRLAVLQHLTHGEHRVRDLTEHTGLAQSTVSAHLACLKDCGLVDSRPQGRASMFFLTTAPELLAVL
AAAELLLDATGHAVELCPTYGLGRTGSQTSMPANTSTHEESTR

Blast result :

ORF 1

Length		Begin	End	Strand	Fusion ORF
960 bp	319 aa	48	1007	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPVAGVDYPRTHQQLSAWFSDEDACLEYLARLRWPDGFRCPSCGGDAAWRTARRALWMCAGCGRQTSVTAGTIFHRSHTPLTTWFAAIWFVCASKNGVSA
STLQQVLGFGSYETAWAWLHKLRRVMVRPDRDLLGGVVELDETLVGGRAKPASSVVITKVPVMVAVETPAGHQLGRIRLARADARNSGQLIDFAHQVIAP
GTLIRTDGARNLRRLAEQGYTHEYKVMLTSPDPAHEYLPGVHLVASLLKRWVAGTLHHGISRRQLDYYLDEFTFRFNRRSSRSRGLLFYRLLQQAVATDP
HPLAALVGGTDQDRGGGAS

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
1959 bp	652 aa	2972	1014	-	No

ORF function : Passenger Gene
Annotation : Cadmium-translocating P-type ATPaseDescription :

ORF sequence :

MTDDRGADQTTADEIDDDDDKDVTSIWGVSEIRAGLVAGTLLLVAFLLGLGGLHGWALGLNIAALLVGASTFVPETLQKLARGKIGVGTLMTIAAVGAVI
LGEFAEAATLAFLYSLSEGLEEYSLAKTRGGLRALLSLVPDTATVRRAGIEQVIDPDDLAVGDTMLVKPGERLATDGTIRTGRTTLDVSAITGESIPVEV
GPGTQVYAGSINGTGALEVEVTTTSEDNSLARIVHIVEAEQSRKGTTQRLADRVAKPLVPAIMIVATLIAVIGSLLGDPGLWIQRALVVLVAASPCALAI
SVPVSVVSAIGAASRFGVLIKGGAALEALGAIRMIALDKTGTLTANAPTVVAVATTAGHTREQVLAVAGALEARSEHPLARAILTAVDTVADAADVQAVP
GAGLTGTVDGRAVRLGRPGWITAADLAAQVQGMQEAGATAVLVEDDGTVTGAIAVRDELRPEARAVVAELHRHDYNVTMLTGDNTATAYAVAAQAGIADV
HAELRPEDKSTLVAALRTKAPTAMVGDGVNDAPALATADLGIAMGAMGSDVALETADVALMGTDLRALPQAFAHARRARRIMLQNVGLSLTLIAILIPLA
VAGVLGLAAVVLVHELAEVVVIANGVRAGRATNLHTTSTVTSVQSPQRAMAR

Blast result :

Comments

ISAcba1 is 72% aa similar to ISMva1.

References

1] ISfinder annotation (2017)
2] Kantor,R.S., Huddy,R.J., Iyer,R., Thomas,B.C., Brown,C.T., Anantharaman,K., Tringe,S., Hettich,R.L., Harrison,S.T. and Banfield,J.F. (2016) Direct GenBank submission.