ISAlba2

  • Family IS1595
  • Group ISSod11
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MEMC01000040 ND Alphaproteobacteria bacterium
Alphaproteobacteria bacterium RBG_16_42_14
DNA section
IS Length : 1013 bp

Ends


IR Length : 32/35

IRL : GCTGCTTACCCATTTGACTCCGGTGACTAAATTTGCCAGAATGGAGAAAT
IRR : GCTGCTTACCCATTTGACTCCGGTTGCTTAATTTGTCGTAGGGGTGCTGG

Insertion site


Left flankDirect repeatRight flankDR Length
AAAATACCGACANNNNN0

DNA sequence

GCTGCTTACCCATTTGACTCCGGTGACTAAATTTGCCAGAATGGAGAAATGCGCAAGATAGAATATCCTAAAAACCTGCGTGAGTTTCGTCAAAAATTTT
TAAATGATATTGCCTGCTTAGAATATCTATCAGAAAAACGTTGGCCAGAGGGATTTGTCTGCCCCCGATGTTTAAGTAAATCTGCTTGGTTAAATACGAA
GCGCTATATTTATGAATGTCAAGATTGCCGCAAACAAACATCCCCTACCGCAGGAACCCTTATGCATCGCTCTCACGTTCCTATTCAGGAATGGTTTTGG
GCAGCCTATCTTGTAGCCACTCATACTCCTGGGATTAGTGCTTTACAACTTCAAAGACAACTTGGAATTGCTAAATACGATACTGCTTGGTACTTGCTGC
ATCGTTTGCGTAAGGGAATGGTGGATGACTGCCGTACAAAACTTTCTGGGCTTATTGAGGCTGACGAGACAATTATTGGGGGACCAGCAAAGGGCAAGAA
AGGCCGCGGTGTTACCGTCGCACACAACAAATCTCTCGTAATCGGGGCCGTAGAGGTTCGCTCTTATACTAATAAGCAGGGTAAAACACAAGAACGAGCT
GGTAGGGTTAGGCTGGAGGTTATTTCCCACGCTAATGAAACCCTTATCAAGTCCTTTATGTTACAAAACGTTGAGAAAGGCTCGAATATCCGCACAGATG
GATGGAGAGGATACTCCAAAACGGCACTTGCTGATTATAAACATATGCCTCTGGTTCTCGGCTCTCCCCAGAATGCACATCAACTCGCGCCACACATTCA
TCGTGTCTTTAGCAATCTCAAAACTTGGTTGAATGGTACACACCATAGCGTTGATCCAAAGTATCTTCAAAGTTACTTGAACGAGTACGTTTTCCGATTT
AATCGTCGCATAATCCCTATGGCCGCCTTTCAAACCCTCCTCAAGATTTCATCCCAGAAAAATCCAGCACCCCTACGACAAATTAAGCAACCGGAGTCAA
ATGGGTAAGCAGC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
960 bp319 aa491008+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKIEYPKNLREFRQKFLNDIACLEYLSEKRWPEGFVCPRCLSKSAWLNTKRYIYECQDCRKQTSPTAGTLMHRSHVPIQEWFWAAYLVATHTPGISALQ
LQRQLGIAKYDTAWYLLHRLRKGMVDDCRTKLSGLIEADETIIGGPAKGKKGRGVTVAHNKSLVIGAVEVRSYTNKQGKTQERAGRVRLEVISHANETLI
KSFMLQNVEKGSNIRTDGWRGYSKTALADYKHMPLVLGSPQNAHQLAPHIHRVFSNLKTWLNGTHHSVDPKYLQSYLNEYVFRFNRRIIPMAAFQTLLKI
SSQKNPAPLRQIKQPESNG

 

Blast result :
Comments
ISAlba2 is 65% aa similar to ISEba1.
References
1] ISfinder annotation (2017)
2] Anantharaman,K., Brown,C.T., Hug,L.A., Sharon,I., Castelle,C.J., Probst,A.J., Thomas,B.C., Singh,A., Wilkins,M.J., Karaoz,U., Brodie,E.L., Williams,K.H., Hubbard,S.S. and Banfield,J.F. (2016) Nat Commun 7, 13219.