ISAsp1

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
U13767 ND Anabaena sp.
Anabaena sp. PCC7120
DNA section
IS Length : 1419 bp

Ends


IR Length : 19/22

IRL : GGTTCTTCCGGTAGTTTTATGGTGTTTATTAGAATTGACTTGATTTTAAT
IRR : GGTTCTTCAGTTAATTTTATGGAGAATTAATAGTAAAATGCCAGTTTAAC

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

GGTTCTTCCGGTAGTTTTATGGTGTTTATTAGAATTGACTTGATTTTAATAACTTATAATTTAGTGATATCAATGCCTATAAGATAGATTACATAGGCAT
TTAGTAAATATCAAGCATAGTTAAAGAACTCTAAAATTATCATCTATATTATAATATAGTAAAGTTTTAGGCGATCGCTATGAAGTTTCCAGTAGAACAA
ATCCTGAATCTTCCCGATATGAAAGTATTAGATTTTCAAGAACTTGAAGGTGAAGAAATAATTATAACGATAGAAAAAAGTGTGAACTATTCGACTTGTC
CTTGGTGTGGTCAAATGACTCACAGTATACATCAAAATCACTGGCGAATAATTCATGATTTACCGTGGAATAAAAAACCAGTGTTATTAAAAATAAATCG
TCGGCAATTTAAATGTCATAAGTGTAAAAAAGTATTTAGCGAACAACTAGACTTTGTAGATAAAAGTAAAGGATATACTAAAAGATTAGCGACCGACATA
GTTCAACAAGTATTAAATAGTAATATCCGGAGTGTTGCCCAAAGAAATGGATTAAGTGATGAAGAAGTAGAATCAATGTTAAAAAAGCAAGTCTCGCACA
TATTAAACATTAACCTAAGTCAGGTAAAAAAGTTAGGTATAGATGAAATAGCTTTAGTTAAAGGAAAAGGAAACTACTTAGCAATATTAGTGGATTTAGA
TAGTCATAAACCAATTGAGATAGTGCAATCAAGACGAATAGAAGATATTCGTGAAGTACTTGTCAGAAGGGGAGTTGAGATACTTAAGGTCAAAATTGAA
GAAGTGAGCATTGACCTCTGGTTGCCTTATAAAAATTTAGTAGAAGAGTTAATGCCAAATGCTAATATAACTGCTGATAGATTTCATGTAATGAAACAAG
TAAATGACGAATTAGATACTATGCGTAAAGCTGAAAAGAAAGCAGCTATATCGCTAGATAATAAATCAGAAAAAGAGCGAATATTAGAGGCATTGAATAA
GAGAAATATGTCGATTATTAAAAATGAAGACTCTCTAAATGAAAAGCAAAGAGAAAAATTAAAATCTGTGCAAGAGGTGTCGCCAAATCTCGCCAAAATG
CACTCACTTAAAGAACAATTTCGAGATATATTTGAAACAACTAAATCCTGGGGAGATAGTATAATAAAACTATTGGATTGGATGTATGATGCACTTTCAT
ATTTCCCGAAAAGTATAGGTACAATAGTTAGATGGTTTGGTGAAATAGTCGGTTATTTTGACGGAAGAACCACAAGTGGTGCTGTAGAAGGAATTAATAA
TAAACTCAAGTTAATTAAAAGACTTGGATATGGCTTTCGTAACTTTACCAATTTCAGATTACGCAGTTTGTTAAACTGGCATTTTACTATTAATTCTCCA
TAAAATTAACTGAAGAACC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1224 bp407 aa1801403+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MKFPVEQILNLPDMKVLDFQELEGEEIIITIEKSVNYSTCPWCGQMTHSIHQNHWRIIHDLPWNKKPVLLKINRRQFKCHKCKKVFSEQLDFVDKSKGYT
KRLATDIVQQVLNSNIRSVAQRNGLSDEEVESMLKKQVSHILNINLSQVKKLGIDEIALVKGKGNYLAILVDLDSHKPIEIVQSRRIEDIREVLVRRGVE
ILKVKIEEVSIDLWLPYKNLVEELMPNANITADRFHVMKQVNDELDTMRKAEKKAAISLDNKSEKERILEALNKRNMSIIKNEDSLNEKQREKLKSVQEV
SPNLAKMHSLKEQFRDIFETTKSWGDSIIKLLDWMYDALSYFPKSIGTIVRWFGEIVGYFDGRTTSGAVEGINNKLKLIKRLGYGFRNFTNFRLRSLLNW
HFTINSP

 

Blast result :
Comments
ISAsp1 ORF is 25 % identical to the putative transposase of ISPp2 from Pseudomonas putida ML2 (in a 373 aa overlap). ISAsp1 is located 280 bp downstream of the hlyA gene (Bauer et al., 1994).
References
1] Bauer, C.C., Ramaswamy, K.S., Endley, S., Golden, J.W., and Haselkorn, R. (1994) "Suppression of heterocyst differentiation in Anabaena sp. strain PCC 7120 by a cosmid carrying wild-type genes encoding enzymes of polyketide or fatty acid synthesis". EMBL Data Library, Rel. 41.