ISAsp1
- Family ISL3
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| U13767 | ND | Anabaena sp. | Anabaena sp. PCC7120 |
DNA section
IS Length : 1419 bp
Ends
IR Length : 19/22
IRL : GGTTCTTCCGGTAGTTTTATGGTGTTTATTAGAATTGACTTGATTTTAAT
IRR : GGTTCTTCAGTTAATTTTATGGAGAATTAATAGTAAAATGCCAGTTTAAC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|
DNA sequence
GGTTCTTCCGGTAGTTTTATGGTGTTTATTAGAATTGACTTGATTTTAATAACTTATAATTTAGTGATATCAATGCCTATAAGATAGATTACATAGGCAT
TTAGTAAATATCAAGCATAGTTAAAGAACTCTAAAATTATCATCTATATTATAATATAGTAAAGTTTTAGGCGATCGCTATGAAGTTTCCAGTAGAACAA
ATCCTGAATCTTCCCGATATGAAAGTATTAGATTTTCAAGAACTTGAAGGTGAAGAAATAATTATAACGATAGAAAAAAGTGTGAACTATTCGACTTGTC
CTTGGTGTGGTCAAATGACTCACAGTATACATCAAAATCACTGGCGAATAATTCATGATTTACCGTGGAATAAAAAACCAGTGTTATTAAAAATAAATCG
TCGGCAATTTAAATGTCATAAGTGTAAAAAAGTATTTAGCGAACAACTAGACTTTGTAGATAAAAGTAAAGGATATACTAAAAGATTAGCGACCGACATA
GTTCAACAAGTATTAAATAGTAATATCCGGAGTGTTGCCCAAAGAAATGGATTAAGTGATGAAGAAGTAGAATCAATGTTAAAAAAGCAAGTCTCGCACA
TATTAAACATTAACCTAAGTCAGGTAAAAAAGTTAGGTATAGATGAAATAGCTTTAGTTAAAGGAAAAGGAAACTACTTAGCAATATTAGTGGATTTAGA
TAGTCATAAACCAATTGAGATAGTGCAATCAAGACGAATAGAAGATATTCGTGAAGTACTTGTCAGAAGGGGAGTTGAGATACTTAAGGTCAAAATTGAA
GAAGTGAGCATTGACCTCTGGTTGCCTTATAAAAATTTAGTAGAAGAGTTAATGCCAAATGCTAATATAACTGCTGATAGATTTCATGTAATGAAACAAG
TAAATGACGAATTAGATACTATGCGTAAAGCTGAAAAGAAAGCAGCTATATCGCTAGATAATAAATCAGAAAAAGAGCGAATATTAGAGGCATTGAATAA
GAGAAATATGTCGATTATTAAAAATGAAGACTCTCTAAATGAAAAGCAAAGAGAAAAATTAAAATCTGTGCAAGAGGTGTCGCCAAATCTCGCCAAAATG
CACTCACTTAAAGAACAATTTCGAGATATATTTGAAACAACTAAATCCTGGGGAGATAGTATAATAAAACTATTGGATTGGATGTATGATGCACTTTCAT
ATTTCCCGAAAAGTATAGGTACAATAGTTAGATGGTTTGGTGAAATAGTCGGTTATTTTGACGGAAGAACCACAAGTGGTGCTGTAGAAGGAATTAATAA
TAAACTCAAGTTAATTAAAAGACTTGGATATGGCTTTCGTAACTTTACCAATTTCAGATTACGCAGTTTGTTAAACTGGCATTTTACTATTAATTCTCCA
TAAAATTAACTGAAGAACC
TTAGTAAATATCAAGCATAGTTAAAGAACTCTAAAATTATCATCTATATTATAATATAGTAAAGTTTTAGGCGATCGCTATGAAGTTTCCAGTAGAACAA
ATCCTGAATCTTCCCGATATGAAAGTATTAGATTTTCAAGAACTTGAAGGTGAAGAAATAATTATAACGATAGAAAAAAGTGTGAACTATTCGACTTGTC
CTTGGTGTGGTCAAATGACTCACAGTATACATCAAAATCACTGGCGAATAATTCATGATTTACCGTGGAATAAAAAACCAGTGTTATTAAAAATAAATCG
TCGGCAATTTAAATGTCATAAGTGTAAAAAAGTATTTAGCGAACAACTAGACTTTGTAGATAAAAGTAAAGGATATACTAAAAGATTAGCGACCGACATA
GTTCAACAAGTATTAAATAGTAATATCCGGAGTGTTGCCCAAAGAAATGGATTAAGTGATGAAGAAGTAGAATCAATGTTAAAAAAGCAAGTCTCGCACA
TATTAAACATTAACCTAAGTCAGGTAAAAAAGTTAGGTATAGATGAAATAGCTTTAGTTAAAGGAAAAGGAAACTACTTAGCAATATTAGTGGATTTAGA
TAGTCATAAACCAATTGAGATAGTGCAATCAAGACGAATAGAAGATATTCGTGAAGTACTTGTCAGAAGGGGAGTTGAGATACTTAAGGTCAAAATTGAA
GAAGTGAGCATTGACCTCTGGTTGCCTTATAAAAATTTAGTAGAAGAGTTAATGCCAAATGCTAATATAACTGCTGATAGATTTCATGTAATGAAACAAG
TAAATGACGAATTAGATACTATGCGTAAAGCTGAAAAGAAAGCAGCTATATCGCTAGATAATAAATCAGAAAAAGAGCGAATATTAGAGGCATTGAATAA
GAGAAATATGTCGATTATTAAAAATGAAGACTCTCTAAATGAAAAGCAAAGAGAAAAATTAAAATCTGTGCAAGAGGTGTCGCCAAATCTCGCCAAAATG
CACTCACTTAAAGAACAATTTCGAGATATATTTGAAACAACTAAATCCTGGGGAGATAGTATAATAAAACTATTGGATTGGATGTATGATGCACTTTCAT
ATTTCCCGAAAAGTATAGGTACAATAGTTAGATGGTTTGGTGAAATAGTCGGTTATTTTGACGGAAGAACCACAAGTGGTGCTGTAGAAGGAATTAATAA
TAAACTCAAGTTAATTAAAAGACTTGGATATGGCTTTCGTAACTTTACCAATTTCAGATTACGCAGTTTGTTAAACTGGCATTTTACTATTAATTCTCCA
TAAAATTAACTGAAGAACC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1224 bp | 407 aa | 180 | 1403 | + | No |
Chemistry : Unknow
ORF sequence :
MKFPVEQILNLPDMKVLDFQELEGEEIIITIEKSVNYSTCPWCGQMTHSIHQNHWRIIHDLPWNKKPVLLKINRRQFKCHKCKKVFSEQLDFVDKSKGYT
KRLATDIVQQVLNSNIRSVAQRNGLSDEEVESMLKKQVSHILNINLSQVKKLGIDEIALVKGKGNYLAILVDLDSHKPIEIVQSRRIEDIREVLVRRGVE
ILKVKIEEVSIDLWLPYKNLVEELMPNANITADRFHVMKQVNDELDTMRKAEKKAAISLDNKSEKERILEALNKRNMSIIKNEDSLNEKQREKLKSVQEV
SPNLAKMHSLKEQFRDIFETTKSWGDSIIKLLDWMYDALSYFPKSIGTIVRWFGEIVGYFDGRTTSGAVEGINNKLKLIKRLGYGFRNFTNFRLRSLLNW
HFTINSP
KRLATDIVQQVLNSNIRSVAQRNGLSDEEVESMLKKQVSHILNINLSQVKKLGIDEIALVKGKGNYLAILVDLDSHKPIEIVQSRRIEDIREVLVRRGVE
ILKVKIEEVSIDLWLPYKNLVEELMPNANITADRFHVMKQVNDELDTMRKAEKKAAISLDNKSEKERILEALNKRNMSIIKNEDSLNEKQREKLKSVQEV
SPNLAKMHSLKEQFRDIFETTKSWGDSIIKLLDWMYDALSYFPKSIGTIVRWFGEIVGYFDGRTTSGAVEGINNKLKLIKRLGYGFRNFTNFRLRSLLNW
HFTINSP
Blast result :
Comments
ISAsp1 ORF is 25 % identical to the putative transposase of ISPp2 from Pseudomonas putida ML2 (in a 373 aa overlap). ISAsp1 is located 280 bp downstream of the hlyA gene (Bauer et al., 1994).
References
1] Bauer, C.C., Ramaswamy, K.S., Endley, S., Golden, J.W., and Haselkorn, R. (1994) "Suppression of heterocyst differentiation in Anabaena sp. strain PCC 7120 by a cosmid carrying wild-type genes encoding enzymes of polyketide or fatty acid synthesis". EMBL Data Library, Rel. 41.