ISNesp1
- Family IS1595
- Group IS1016
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LTSK01000089 | ND | Neisseria sp. | Neisseria sp. HMSC056A03 |
DNA section
IS Length : 720 bp
Ends
IR Length : 20/21
IRL : GGGGCTGTACTAGATAACTAGGGAAATCTAATTTTAGGTTAGAATAATCC
IRR : GGGGCTGTACTAGATAACCAGAATAAATTCTGCTTTACTAATTGTTTTAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TAAAAAGATA | TAAAAATA | TTTTTGTTTT | 8 |
DNA sequence
GGGGCTGTACTAGATAACTAGGGAAATCTAATTTTAGGTTAGAATAATCCCTATGAGAAGAAGCCGTTTAAGTCAGTACAAACAAAACAAACTGATTGAG
CTGTTTATTGCAGGTGTTACCGCACGCACAGCAGCGCAATTAGTCAGTGTTAATAAAAACACATCAGCCTATTACTTTCATCGTTTACGTGTACTAATTT
TTCATAACAGCCCGCATCTGGAAATGTTTGATGGCGAAGTAGAAGTTGATGAAAGCTATTTTGGCGGGCAGCGCAAAGGCAAACGCGGTCGCGGTGCAGC
CGGCAAAGTGGCTGTATTCGGACTTCTGAAGCGCAACGGCAAGGTTTACACCGTTGCTGTGCCCAATACACAAACTGCCACTTTATTGCCGATGATACGT
GAGCAAGTAAAGCCTGACAGTATTGTTTATACTGATTGTTACAAAAGCTATGACGTACTTGATGTGAGTGAGTTTAGCCACTTTCGCATTAATCACAGCA
CACATTTTGCTGATCGACAAAATCACATCAACGGAATTGAGAACTTTTGGAATCAGGCAAAACGCCACTTACGTAAGTTTAACGGCATTCCCAAAGAGCA
TTTTGAGCTGTATTTGAAGGAATGTGAATGGCGTTTTAACCATGGTGAAATAAAAGTTCAAATTTCTATTTTAAAACAATTAGTAAAGCAGAATTTATTC
TGGTTATCTAGTACAGCCCC
CTGTTTATTGCAGGTGTTACCGCACGCACAGCAGCGCAATTAGTCAGTGTTAATAAAAACACATCAGCCTATTACTTTCATCGTTTACGTGTACTAATTT
TTCATAACAGCCCGCATCTGGAAATGTTTGATGGCGAAGTAGAAGTTGATGAAAGCTATTTTGGCGGGCAGCGCAAAGGCAAACGCGGTCGCGGTGCAGC
CGGCAAAGTGGCTGTATTCGGACTTCTGAAGCGCAACGGCAAGGTTTACACCGTTGCTGTGCCCAATACACAAACTGCCACTTTATTGCCGATGATACGT
GAGCAAGTAAAGCCTGACAGTATTGTTTATACTGATTGTTACAAAAGCTATGACGTACTTGATGTGAGTGAGTTTAGCCACTTTCGCATTAATCACAGCA
CACATTTTGCTGATCGACAAAATCACATCAACGGAATTGAGAACTTTTGGAATCAGGCAAAACGCCACTTACGTAAGTTTAACGGCATTCCCAAAGAGCA
TTTTGAGCTGTATTTGAAGGAATGTGAATGGCGTTTTAACCATGGTGAAATAAAAGTTCAAATTTCTATTTTAAAACAATTAGTAAAGCAGAATTTATTC
TGGTTATCTAGTACAGCCCC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 668 bp | 222 aa | 53 | 720 | + | No |
Chemistry : DDE
ORF sequence :
MRRSRLSQYKQNKLIELFIAGVTARTAAQLVSVNKNTSAYYFHRLRVLIFHNSPHLEMFDGEVEVDESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYT
VAVPNTQTATLLPMIREQVKPDSIVYTDCYKSYDVLDVSEFSHFRINHSTHFADRQNHINGIENFWNQAKRHLRKFNGIPKEHFELYLKECEWRFNHGEI
KVQISILKQLVKQNLFWLSSTA
VAVPNTQTATLLPMIREQVKPDSIVYTDCYKSYDVLDVSEFSHFRINHSTHFADRQNHINGIENFWNQAKRHLRKFNGIPKEHFELYLKECEWRFNHGEI
KVQISILKQLVKQNLFWLSSTA
Blast result :
Comments
ISNesp1 is 93% aa similar to ISHpa1.
The STOP codon is missing, the transposase use the first STOP codon after the end of the IS. The transposase was in silico deleted of the 8 last amino acids.
The STOP codon is missing, the transposase use the first STOP codon after the end of the IS. The transposase was in silico deleted of the 8 last amino acids.
References
1] ISfinder annotation (2017)
2] Mitreva,M., Pepin,K.H., Mihindukulasuriya,K.A., Fulton,R., Fronick,C., O'Laughlin,M., Miner,T., Herter,B., Rosa,B.A., Cordes,M., Tomlinson,C., Wollam,A., Palsikar,V.B., Mardis,E.R. and Wilson,R.K. (2016) Direct GenBank submission.
2] Mitreva,M., Pepin,K.H., Mihindukulasuriya,K.A., Fulton,R., Fronick,C., O'Laughlin,M., Miner,T., Herter,B., Rosa,B.A., Cordes,M., Tomlinson,C., Wollam,A., Palsikar,V.B., Mardis,E.R. and Wilson,R.K. (2016) Direct GenBank submission.