ISAr1

  • Family IS630
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) Riat1
Accession numberTranspositionOriginHost
K03313 ND Agrobacterium rhizogenes
Agrobacterium rhizogenes HR1
DNA section
IS Length : 1146 bp

Ends


IR Length : 11

IRL : GTGGATTGATTGAAACAAAGGAGTCCGAGTTGGGATTCCCTTTCGGTCTT
IRR : GTGGATTGATTCCAACGCTTGGTGCCCACGTTTGACGGCTGCAATGATCT

Insertion site


Left flankDirect repeatRight flankDR Length
TTGTCTTAACTAGTCCCAATTT2

DNA sequence

GTGGATTGATTGAAACAAAGGAGTCCGAGTTGGGATTCCCTTTCGGTCTTCGTCGTGCAACGATATCGTATGCGTACAGGTATCACATTTAACGTTGCTG
CGGCGGACCGAGCCCGCTTGGAAGCGATTGTTGCAGCTCCAACTTCTGCTCAGAAGCACGTGTGGCGAGCGAAGATCATCTTGATGAGCAGTGATGGCTC
GGGAACGGTCGCGATCATGGAGGCAACCGGTAAATCCAAAACCTGTGTCTGGCGCTGGCAGGAGCGCTTCATGACTGAGGGCGTCGATGGCCTTTTGCAC
GACAAGAGCAGACCGCCCGGCATTGCGCCGCTTGATGGCGAACTCGTTGAGCGTGTCGTCGCACTGACGCTTGAGACGCCTCAACAGGAAGCAACGCACT
GGACTGTTCGTGCGATGGCCAAGGCCGTTGGGATTGCAGCCTCTTCGGTTGTGAAGATCTGGCACGAGCATGGTCTTGCGCCGCATCGCTGGCGCTCTTT
CAAACTGTCGAACGACAAGGCCTTTGCCGAGAAGCTTCACGACGTCGTTGGCCTCTACGTCTCGCCACCGGCCCATGCCATTGTCCTGTCCGTCGATGAG
AAGAGCCAGATCCAGGCACTCGATCGGACGCAACCGGGACTCCCCTTGAAGAAAGGGCGCGCCGGCACAATGACCCACGATTACAAGCGCCACGGCACCA
CCACCCTATTTGCCGCCCTCAACATCCTCGACGGCTCGGTGATCGGCCGAAACATGCAGCGTCACCGGCATCAGGAGTTCATCCGTTTTCTCAACGCCAT
CGAGGCGGAACTGCCAAAGGACAAGGCCGTCCACGTCATTCTCGACAATTACGCGACCCATAAGCAGCCGAAGGTCCGCGCCTGGCTGGCAAGGCATCCG
CGCTGGACCTTCCACTTCGTCCCAACATCATGTTCATGGCTGAACGCCGTCGAGGGATTCTTCGCTAAATTGACACGTCGACGTCTGAAGCACGGTGTCT
TTCATTCCGTCGTTGACCTCCAGGCCACCATCAACCGCTTCGTCAGAGAGCATAATCAGGAACCAAAGCCGTTCATCTGGAGAGCAGATCCAGACGAGAT
CATTGCAGCCGTCAAACGTGGGCACCAAGCGTTGGAATCAATCCAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1080 bp359 aa691148+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRTGITFNVAAADRARLEAIVAAPTSAQKHVWRAKIILMSSDGSGTVAIMEATGKSKTCVWRWQERFMTEGVDGLLHDKSRPPGIAPLDGELVERVVALT
LETPQQEATHWTVRAMAKAVGIAASSVVKIWHEHGLAPHRWRSFKLSNDKAFAEKLHDVVGLYVSPPAHAIVLSVDEKSQIQALDRTQPGLPLKKGRAGT
MTHDYKRHGTTTLFAALNILDGSVIGRNMQRHRHQEFIRFLNAIEAELPKDKAVHVILDNYATHKQPKVRAWLARHPRWTFHFVPTSCSWLNAVEGFFAK
LTRRRLKHGVFHSVVDLQATINRFVREHNQEPKPFIWRADPDEIIAAVKRGHQALESI

 

Blast result :
Comments
The ORF stop codon is the TAG of the CTAG target site. ISAr1 transposase is 89 % identical to that of IS870. A duplication of two bp have been considered here, although this statement needs confirmation. Coordinates on the plasmid Ri : 5071-6217.
References