ISAxf1

  • Family IS1595
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JXYS01000006 ND Acidithrix ferrooxidans
Acidithrix ferrooxidans Py-F3
DNA section
IS Length : 1040 bp

Ends


IR Length : 22

IRL : CATACTTATCAAGTTTCGTAAGTTTCGCCTCCACCGAGACCAGATTGGAG
IRR : CATACTTATCAAGTTTCGTAAGCAAGAGCCATTTTGGAATCAGATTCGCG

Insertion site


Left flankDirect repeatRight flankDR Length
AAGGGTAACTGGTTCCGAAACTACCCATTGGG8

DNA sequence

CATACTTATCAAGTTTCGTAAGTTTCGCCTCCACCGAGACCAGATTGGAGCTAATCTTTAGTAGAACAGATGTTCGATAAAGGAGGCATGGTGGATATCT
CAGGCTTTATGGTACGCTTCGCTTCAGAGGAAGCATGTGAAGATCATTTGATCAAATTGCGGTGGAAAGAGGGATTTAGATGCCCAAAGTGTGACTCTGA
CCAGTTTACCTTAATCAGGCCAAACCATCGAAGAAATGCAGCATCTCGAGCTCCACTTTTTCAATGTAAATCTTGTCACCGTCAAACTAGCGTTACCTCC
GGAACCATCTTCCATCGAAGCCATATCTCTTTATCCAAGTGGTTCTCCGCTATCTATCTATTGTCCAACGACAAGCGTGGTCTTTCGGCTACTACCATTG
CTAAATTCGTTCAAGTATCTTATTCGACTGGCTGGTTGATGCTAAATAAGCTACGAAAAGCAATGGCTGACCGAAATGGTTTATATAAACTTGGAGGAAA
CGTCCAGGTAGATGAGTTCTTTTTAGGTGGTGAGAGCCATGGCGAAGGCAGAAGAGGAAAGGGAACAAAACAAACCAATGTCCTAATTGGAGTGTCGATT
AGCAATGGTGCTCCTACTCATTGCTTCATGGAAGTAATCAAAGATACCACTGCTAAATCATTCAAAGATGTTTTTGTGAGACGTTTAGATTCCGATACCA
AGCTGATAAGTGATGGTAACCCAAGTTACGGGGTATGTGCTCGAGACCTTGGACTAGCGCATTCGATCACTCTATCCAAAGACGAACAAGCACATGTAAC
ATTCAAATGGCTCAACATCCTGATAGGGAACTGCAAGAAATTTATCGACGGAACATACCACGGACGTGAGGAACATAAGCAACTTTACCTCGAGGAATTC
GCATACCGATTCAACCGAAGGCACTTTGAAATGTCCTTGGTAGAGCGACTTCTCAATACATGTGTGTTTGCGTCTCCACATCCGCTACTCCGCGAATCTG
ATTCCAAAATGGCTCTTGCTTACGAAACTTGATAAGTATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
942 bp313 aa911032+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MDISGFMVRFASEEACEDHLIKLRWKEGFRCPKCDSDQFTLIRPNHRRNAASRAPLFQCKSCHRQTSVTSGTIFHRSHISLSKWFSAIYLLSNDKRGLSA
TTIAKFVQVSYSTGWLMLNKLRKAMADRNGLYKLGGNVQVDEFFLGGESHGEGRRGKGTKQTNVLIGVSISNGAPTHCFMEVIKDTTAKSFKDVFVRRLD
SDTKLISDGNPSYGVCARDLGLAHSITLSKDEQAHVTFKWLNILIGNCKKFIDGTYHGREEHKQLYLEEFAYRFNRRHFEMSLVERLLNTCVFASPHPLL
RESDSKMALAYET

 

Blast result :
Comments
ISAxf1 is 61% aa similar to ISPaen7.
References
1] ISfinder annotation (2017).
2] Eisen,S., Poehlein,A., Johnson,D.B., Daniel,R., Schlomann,M. and Muhling,M. (2015) Genome Announc 3 (2).