ISAe1

  • Family ISL3
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s) ISAE1
Accession numberTranspositionOriginHost
M86608 Y Ralstonia eutropha
Ralstonia eutropha ATCC17699 plasmid pHG1
Ralstonia eutropha H1-4
Ralstonia eutropha ATCC17698 plasmid pAE1
DNA section
IS Length : 1313 bp

Ends


IR Length : 18/24

IRL : GCCTCTTTTGAATTTCAAGTGGGTGGTATCGTGGCGGGTTTCAGGTAAAG
IRR : GGCTCTTTTGAAAAATGAGTGGGTTATGCGGCATGCGGGTTGATCGCGGA

Insertion site


Left flankDirect repeatRight flankDR Length
NNNNNNNNNNATAAGAATNNNNNNNNNN8
NNNNNNNNNNCAAAAACCNNNNNNNNNN8
NNNNNNNNNNATAGAGAGNNNNNNNNNN8
NNNNNNNNNNATAGAGAGNNNNNNNNNN8
NNNNNNNNNNCAACAAGANNNNNNNNNN8
NNNNNNNNNNAATCTTTTNNNNNNNNNN8
NNNNNNNNNNAATCTTTTNNNNNNNNNN8
NNNNNNNNNNTAGTTTCANNNNNNNNNN8
NNNNNNNNNNTCTTTTCTNNNNNNNNNN8

DNA sequence

GCCTCTTTTGAATTTCAAGTGGGTGGTATCGTGGCGGGTTTCAGGTAAAGAGCAACAAGAGCGATGCATAGCAAACTGTTTGAAGCAGCCCTTGGCATAA
GCGATCCGTGGTTCGTCCGGGAAGTCGACTTCAACGCGCAAACCAAGACGTTGACGATCCAGATTGACTTTGTCGCCGGCAGCCGCTTTTCCCACCCGGA
GGTCGCCGGTGGGCACCCGGTTCACGACACGGTGACCAAACGCTATCGGCACCTGAACTTCTTCGAGCACGACTGCTATCTGGAAGTCCGCACGCCACGC
GTGAAGCTTCCCGACGGCCGCGTGGTGTTGGTCGAGCCGGACTGGGCTGGCAAGCTCTCTGGCTTCACGTTGCTGTTTGAAGCCATGGTGGTGGCGCTTG
CGCAGCAGATGCCGTTTTCTGCTGTAGCCAGAACGGTGGGTGAGTCGTGGCATCGCGTGTACGCTATCTGCGAGCGCTATGTCGACCTCGCGGTGGCCGA
ACTCGATCTGGCCGGCGTGACCGCTGCCGCCGTTGACGAAACGTCCTACAGGCGCGGTCACAACTACCTGACGCTTGTCGCTGATGCCGACGCGCGTAAG
GTCGTGTTTGTGACCGAAGGCAAGGATGCTGCCACGGTTGGCAAGTTCGCTGCACATCTGCGCGAGAACAACGCTGCGCCTGAGCAGATCGGGGTGGTCA
GTATCGACATGTCGCCGGCGTTCATCAAGGGCGTCAGCGAGCATTTGCCCAACGCGCGCATCACCTTCGACAAGTTCCATGTCGTGGCTCACGCTTCTGC
AGCCGTGGACAAGACCCGGCGTATCGAACAGAAGACCGATCCAAGCCTCAAAGGCCTGCGCTGGACGCTGCTCAAGGATCGCGACGGGTTGCCAGCCGCG
CAGCGTGCCGATCTCGACGCGTTGATCGCCAACGTCACGACCAAACGCACTGCCCGTGCCTGGCTGTATCGCGAGCAGTTGCGCGAAATCCTTGAGCGCA
AGCAGATCAACGTTGTTTCCGCAATGCTCGAGCAGTGGTGCACGAACGTCATGCGATCCAAGGTCGAGCCAATGAAGGAGGTCGCGCGCATGATCCGCAA
GCATTTCGACGGTATCGTTGCATGGACCCAGACGCGCCAGACCAATGGCTTCCTGGAGGCGCTCAACGGCCTGTTCCAAGCCGCCAAGCGCAAGGCCCGC
GGTTATGTGAGCTTCAAAACCATGCGCACCGTGATCTTCCTGATCGCCGGAAAGCTCGACTTCTCCGCGATCAACCCGCATGCCGCATAACCCACTCATT
TTTCAAAAGAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1227 bp408 aa641290+No
ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MHSKLFEAALGISDPWFVREVDFNAQTKTLTIQIDFVAGSRFSHPEVAGGHPVHDTVTKRYRHLNFFEHDCYLEVRTPRVKLPDGRVVLVEPDWAGKLSG
FTLLFEAMVVALAQQMPFSAVARTVGESWHRVYAICERYVDLAVAELDLAGVTAAAVDETSYRRGHNYLTLVADADARKVVFVTEGKDAATVGKFAAHLR
ENNAAPEQIGVVSIDMSPAFIKGVSEHLPNARITFDKFHVVAHASAAVDKTRRIEQKTDPSLKGLRWTLLKDRDGLPAAQRADLDALIANVTTKRTARAW
LYREQLREILERKQINVVSAMLEQWCTNVMRSKVEPMKEVARMIRKHFDGIVAWTQTRQTNGFLEALNGLFQAAKRKARGYVSFKTMRTVIFLIAGKLDF
SAINPHAA

 

Blast result :
Comments
Note that Ralstonia eutropha was formerly named Alcaligenes eutrophus. Evidences are presented that the ISAe1 transposition is stimulated or selected for by the growth of bacteria under unfavourable conditions (temperature shift). Transposition events have been deduced from variations of the IS patterns in several strains.
References
1] Kung, S.-S., Chen, J., and Chow, W.-Y. (1992) J. Bacteriol. 174, 8023-8029.