ISAcpr1

  • Family IS1595
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JQSG02000001 ND Acidihalobacter prosperus
Acidithiobacillus caldus ATCC 51756
Acidihalobacter prosperus DSM 5130
Acidithiobacillus caldus ATCC 51756 plasmid megaplasmid mpAca1.1
DNA section
IS Length : 977 bp

Ends


IR Length : 23/28

IRL : CATGCCTCTGAACCATTCTCAGCAACAAAGCTCTAACTCGTTGTGAAAAA
IRR : CATGCCTATTAATCTTTCTCAGCAACAAGTCGGACGGGCAGATGGTCCAT

Insertion site


Left flankDirect repeatRight flankDR Length
TTGTGGCACTTTATTAAAATGAAATATG8
GGCTTCCAGCTCTTGGCAAAATAAAATTCGTGAATGATGATCTCTTCA0
CCAGAGATGAGTCCACCAAGATCCAGAGATGAGTTCCACCAA0
CATCGGGCTTATCATTTTAGAATATCTGTCGATCCTGGCGGTG8
AGGTTTCGCGGCAATACCAAAAAGCGCGGTGCTCG8

DNA sequence

CATGCCTCTGAACCATTCTCAGCAACAAAGCTCTAACTCGTTGTGAAAAATGGAGAAAGGAGCCCTGAGATGAAAGCTGAAGCGATGAACCTCGTGCAAT
GGCAAGCGCGGTTTGGCACGGAAGAGGCCTGCTTGGAGGCGCTGAAACAAAAGCGCTGGCCCGAGGGGTTTCAATGCCCCAAGTGCGGGCATGACCGCGG
ATACTGGATCGCTGGACGAAAACTCTTTCAGTGCGGGCACTGTCGCCATCAGACCTCGGTGACGGCCGGAACGATTTTTCATTCGTCCAATGTGCCCCTG
GTGCAATGGTTCCTGGCCATCTATTTGATGGCGAGCGACAAGGGCGGACTGTCGGCCCTGCGGCTGTCGAAGCAGATTGGGGTCTCCTGGATCACGGCTC
ACCGGATGTTGCGCCGCATGCGCCGCGCCATGGGCGATCGCGACAGCTTGTATCGCTTGGAAGGGCTGGTGGAGTTGGACGATGCCTTTGTCGGTGGACG
CCGGTCTGGCGGGAAGAGCGGTCGAGGGGCCGAAGGCAAGACACCGATCCTGGTTGCCGTGGAAAACCGGGGCAAGAAGGCGGGCTTTATTGCCATAGAG
ACGACGCCTTCGGTCTCTGCCGACCGGATTCGCGAATTCGCGCGGCGACGCTTGCGCCCCAAGCAACCTACCCGTACCGACGGCTTGATAGCGCTGCGGG
TTCTCGGAGAGAGCCAGGAGCATGAGGGGCGCGTGACCAAATCGCAACAGGTGGACGAATGGTTGCCGTGGGTACACATCGCCATTGGTAACCTCAAGGC
CTTTCTTTTGGGCACATTCCATGGGGTATCCGGCAAATATCTGCAGGAATATCTGAACGAATTCGTCTATCGCTTCAATCGCCGTTTCTGGGAGCCAGAA
CTGCCGTTGAGGCTGCTCAATGCCTGTATGGACCATCTGCCCGTCCGACTTGTTGCTGAGAAAGATTAATAGGCATG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
885 bp294 aa85969+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNLVQWQARFGTEEACLEALKQKRWPEGFQCPKCGHDRGYWIAGRKLFQCGHCRHQTSVTAGTIFHSSNVPLVQWFLAIYLMASDKGGLSALRLSKQIGV
SWITAHRMLRRMRRAMGDRDSLYRLEGLVELDDAFVGGRRSGGKSGRGAEGKTPILVAVENRGKKAGFIAIETTPSVSADRIREFARRRLRPKQPTRTDG
LIALRVLGESQEHEGRVTKSQQVDEWLPWVHIAIGNLKAFLLGTFHGVSGKYLQEYLNEFVYRFNRRFWEPELPLRLLNACMDHLPVRLVAEKD

 

Blast result :
Comments
ISAcpr1 is 91% aa similar to ISAth1.
References
1] ISfinder annotation (2017).
2] Ossandon,F.J., Cardenas,J.P., Corbett,M., Quatrini,R., Holmes,D.S. and Watkin,E. (2014) Genome Announc 2 (5).