ISPsy41

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP012179 ND Pseudomonas syringae
Pseudomonas syringae pv. actinidiae
DNA section
IS Length : 2576 bp

Ends


IR Length : 13

IRL : TGCGCATTCCACGCCATCCGGCCACCCAGTCCACCAACATCCGGCCACCT
IRR : TGCGGATTCCACGGTCATCCGGCCACCCATTCCATGAACATCCGGCCACT

Insertion site


Left flankDirect repeatRight flankDR Length

DNA sequence

TGCGCATTCCACGCCATCCGGCCACCCAGTCCACCAACATCCGGCCACCTATTCCACGCTCATCCGGCCAGGCAGTCGGAGCGCAGCGACGCAGGTTCGC
ATTGTTAGTCTGAAGTCCCTGTCGTCGTCAATTTCGTTGCGCGCCTGCGCATCGATTCGCCCTTCAACTCGATTCGATAAGCGTTATGCACCAGCCGGTC
GAGGATCGCATCGCCCAAGGTCGGGTCGCCGATCAGCGCATGCCATTTGTCCACCGGCATCTGGCTGGTGACCAGCGTCGAGCGGTTGCCGTAGCGGTCA
TCCAGCAGCTCAAGCATGTCGCGCCGTTGGGCGGCGGTAAACGGTGCCAGGCCCCAGTCGTCCAGGATCAGCAGGTCAGTCTTGGCGTAGCCGGCCATCA
GTTTGGCGAAGCGGCCGTCGCCGTGGGCCAGGCCCAGTTCCTCCATCAAGCGCGGTAGACGCAGGTAACGCACGCTGTAGCCATCTCGGCAGGCCTTGTG
GGCCAGCGCGCAGGCGAGCCAGGTTTTGCCCACGCCGGTGGGGCCGCCGATGATCAGGTTCAGGCCATCGCGCAGCCATTGGCCGCTGCCCAGTTGCAGG
ATCAAGGCCTTGTCCAGGCCGCGCGGGCTGCGGTAGTCGATGTCTTCCAGGCAGGCGTTGTGGCGCAGTCGCGCGGCTTTCAGGCGAGTCGTCAGACGTG
CGTCTTCGCGCTCGGTCAGCTCGCGGTCGACCATCAGACCGAGGCGTTCGTCGAAGCTGAGATCGTTGATGTCAGGCGTTGCGTGTTGTTCGCCAAGCGC
CTTGATCATGCCGTGCAGGCGCAGGGTTTGCAGCTTGTCGAGCGTTGGATTCGGTAGCATTTCGAAGCTCCTTTTTCAGGTCAGTGGTAGTAGCCAGGGC
CGCGCAGGTTGATGTGCTCGTCGGGCAGCAGCGGCAGGTTTTGCTGGGACAGCGGTAGCTTTTCCAGCCCTTGGCGCAGGATCGATTCGAGGCTTTTGTA
GCTGCATGCGCCCAGTGCGAGCGCACGCTGGCACGCGGCCTCCAGCCGCGCTTCGCCGTGCTGTTTGCTCAGGCGCAGGATGCCCAGGCAGGCGCGGAAG
CCGTGTTGCGGATGGATTCGGCGTTCGAGGATGTAGGTGATGACGCCAGCGGTGTTGGGCCCGGTCTGCTCTGCCCAGCGGATCAGCCGTTGCGGTGTCC
ATTCGGCGTGCTCGCGGTGGCTCTTGGGCATGTGCTCGGTTTGGGTGGTGTGCCGGCCTTTGTGCTGCGAGCGAAGGTGGCTGGCCACGCGCTGGTTGGC
GTGGAAGCACTCGACGGTCTGGGCGGTGAGTCGTACTTCGAGTTGGTGCTTCACCAACTGGTATGGCACCGAGTAAAAGTGGCCGTCGACCTCGACGTGG
TAGTCGATATGGACGCGTACTTTTTTCCATTCGGCGTAGACGTACGGATGCTCCGGTAGCGCTTGCAGCGCCGGTTGGTCGATGGCCTCGAAGGCTGACC
GGCGCGAGCCGGGCAGCTTTTTGAAGGGCTTGTGGTTGAGGCGATCCAGCAGTAGCGCGATGGCTGTGTTGAGTTCGCCAAGCGAGAAAAACTGGCGGTT
GCGCAGCACCGCCAGGATCCAGCGCTCGACCACTTGAACACCGACTTCGACCTTGGCTTTGTCCTTGGGTTTTCGTGAGCGAGCAGGCAAGACGGCGATG
CCGTAATGCTCGGCCAGGTCGCGGTAACTGGGGTTGATGTCGGGCTCGTAACGATGAGCTTTAGTCACGCCACTGCGCAGATTGTCTGGTACCAGGATCT
GCGTTGTGCCGCCGAAAAAAACAAAGCATCGGACGTGCGAGCCCAGCCAGTCGGGCAGTTTCTGCGACCAGGTGGCCTCGGCAAAGGTGTAGCTGGAGGC
GCCGAGGACAGCGCCGAAGATCTGCGCCTGACGGATCTCGCCGGTTTGCCGGTCGATGATCGGCGCTGTTTGCCCGGCGTAATCGACGAACAGCTTTTCG
CCAGCACGATGCTCCTGGCGCATGACCACGTCGACCTTGGCGGCCCAAAGGCGGTAATGCTCGCAGAACCAGCTGTATTGGAAGCCTTGCGGGTGAGCGA
GTCGATATTCCTGCCAGAGCAAGGCCAAAGTGACGCCGGGACGGCGCAACTCGGCATGGACATGAGCCCAGTCAGGCATCGGACGTTGATCACTGGGCAC
ATCGGGCGGCGGCGGAAAAAGATGCCGTTGCAGCTCGGAGTCGGTCAGCACAGACGGCCAGGTCAGGCCGCTGGCAGCAAAGCGATTGAGGTATTCACCG
ACGCTGGCTCGACCTACTTGCAAGCTGGCAGCAACCTGGCGGGCAGACAGGCCGACCTCGAATTTGAGACGTAGCACTTCTCGAATCTTACGCATGGATA
ACCGCTCCACGACGACCTCTCTGCTTCGATAAAAGAGGTCGATGGTAGTGAATTAAACCTGCGTTGCCGCCTGCCTTATGGGTGGCCGGATAGCCGTGGA
ATGGGTGGCCGGATCATCGTGGAATCAGTGGCCGGATGTTCATGGAATGGGTGGCCGGATGACCGTGGAATCCGCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1515 bp504 aa1821696+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRKIREVLRLKFEVGLSARQVAASLQVGRASVGEYLNRFAASGLTWPSVLTDSELQRHLFPPPPDVPSDQRPMPDWAHVHAELRRPGVTLALLWQEYRLA
HPQGFQYSWFCEHYRLWAAKVDVVMRQEHRAGEKLFVDYAGQTAPIIDRQTGEIRQAQIFGAVLGASSYTFAEATWSQKLPDWLGSHVRCFVFFGGTTQI
LVPDNLRSGVTKAHRYEPDINPSYRDLAEHYGIAVLPARSRKPKDKAKVEVGVQVVERWILAVLRNRQFFSLGELNTAIALLLDRLNHKPFKKLPGSRRS
AFEAIDQPALQALPEHPYVYAEWKKVRVHIDYHVEVDGHFYSVPYQLVKHQLEVRLTAQTVECFHANQRVASHLRSQHKGRHTTQTEHMPKSHREHAEWT
PQRLIRWAEQTGPNTAGVITYILERRIHPQHGFRACLGILRLSKQHGEARLEAACQRALALGACSYKSLESILRQGLEKLPLSQQNLPLLPDEHINLRGP
GYYH

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
756 bp251 aa17172472+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MLPNPTLDKLQTLRLHGMIKALGEQHATPDINDLSFDERLGLMVDRELTEREDARLTTRLKAARLRHNACLEDIDYRSPRGLDKALILQLGSGQWLRDGL
NLIIGGPTGVGKTWLACALAHKACRDGYSVRYLRLPRLMEELGLAHGDGRFAKLMAGYAKTDLLILDDWGLAPFTAAQRRDMLELLDDRYGNRSTLVTSQ
MPVDKWHALIGDPTLGDAILDRLVHNAYRIELKGESMRRRATKLTTTGTSD

 

Blast result :
Comments
ISPsy40 is 97% (ORFA : the transposase) and 98% (ORFB : the helper of transposition) aa similar to ISPre4.
References
1] Butler M. (2017) Direct submission to ISfinder
2] Butler M.I., Stockwell P.A., Black M.A., Day R.C., Lamont I.L. and Poulter R.T. (2013) PLoS ONE 8 (2), E57464