ISAca1
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| AF121266 | ND | Acinetobacter calcoaceticus | Acinetobacter calcoaceticus |
DNA section
IS Length : 1186 bp
Ends
IR Length : 18/21
IRL : GGTAATCCCCCCGAAAAATAAAAGGATGTAAAAGTAGAATTTTCTCTTAA
IRR : GGTAATCCTCCCATAAATAACCGAAGTTAAAAGTAGAGGTTAAGCAGCCA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGCGAGAAGC | ACCA | TAGCGTTCGC | 4 |
DNA sequence
GGTAATCCCCCCGAAAAATAAAAGGATGTAAAAGTAGAATTTTCTCTTAAGATACAAGCAGGAGATTCTGCATGAAAACATCGAAATTTACTGACAGTCA
GATCATGTCCATCTTGAAGCAGGCGGAATCTGGCACACCTGTAGCCGCCCTTTGTCGTGAACACGGCATGAGTAATGCCACGTTTTATAAATGGCGTGCC
AAATATGGCGGTATGGATACCTCATTGATGGCTCGACTCAAAGAACTGGAAGCAGAAAATACCAGACTTAAAAAGATGTATGCGGAAGAACGATTAAAGG
CAGAGATCATCCAGGAAGCGATGGCAAAAAAGTGGTGAGGCCATCTTGCCGACGTAAGATGGCACAACAGGCAGTTGCCCACTATGCCATTAGCATTCGT
TTGGCTTGCAGCGCATTTAGTATCAGTGAAACCTGCTACCGTTATCAGGCTAAACTCAGTGATGACAATGCACTCATTGCAGAACAGCTCATTGAACTCA
CTGAAGAAAATACAGATTGGGGCTTTGGTCTGTGTTTCTCTTATCTACGTCATGTTGAAAATTATGTTTGGAATCACAAGCGTGTTTATCGTATTTACTG
TGAATTAGCACTGAATCTGCGTATTAAGCCAAGAAGAAGATTGAAACGACATGCACCAGAACCACTGAAAGAGCCGATCCGGGCAAATCAGGTCTGGTCA
CTGGATTTTATGCATGACCAGCTGATCGATGGTCGTAAGTATCGGCTATTGAATGTAATTGATGATTACCGTCGAGAAGGTTTAGCGATTGAAGCAGGAT
TCTCATTACCGACAATCAGGGTTATTCGTACGTTGAATCAGTTGCTGGAGTGGCGAGAAAAGCCGTTAGTTATTCGGTGTGACAATGGCCCGGAGTTTAT
CAGTCATGAATTTGTACGCTGGGCAACTGAACAAGGTATTCGTATTGAATATATTCAACCAGGTAAGCCACAGCAGAATGCGTATATTGAACGTTATAAT
CGGACTATACGCTATAGCTGGCTAAGCAAGCATCTATTTGACACACTGGAAGAAGTGCAAGATTATGCAACGAGTTGGCTGTGGCATTACAACCATGAAA
GACCACACCAAGCAAACAAAGGAAAGCCACCTCTAATGGCTGCTTAACCTCTACTTTTAACTTCGGTTATTTATGGGAGGATTACC
GATCATGTCCATCTTGAAGCAGGCGGAATCTGGCACACCTGTAGCCGCCCTTTGTCGTGAACACGGCATGAGTAATGCCACGTTTTATAAATGGCGTGCC
AAATATGGCGGTATGGATACCTCATTGATGGCTCGACTCAAAGAACTGGAAGCAGAAAATACCAGACTTAAAAAGATGTATGCGGAAGAACGATTAAAGG
CAGAGATCATCCAGGAAGCGATGGCAAAAAAGTGGTGAGGCCATCTTGCCGACGTAAGATGGCACAACAGGCAGTTGCCCACTATGCCATTAGCATTCGT
TTGGCTTGCAGCGCATTTAGTATCAGTGAAACCTGCTACCGTTATCAGGCTAAACTCAGTGATGACAATGCACTCATTGCAGAACAGCTCATTGAACTCA
CTGAAGAAAATACAGATTGGGGCTTTGGTCTGTGTTTCTCTTATCTACGTCATGTTGAAAATTATGTTTGGAATCACAAGCGTGTTTATCGTATTTACTG
TGAATTAGCACTGAATCTGCGTATTAAGCCAAGAAGAAGATTGAAACGACATGCACCAGAACCACTGAAAGAGCCGATCCGGGCAAATCAGGTCTGGTCA
CTGGATTTTATGCATGACCAGCTGATCGATGGTCGTAAGTATCGGCTATTGAATGTAATTGATGATTACCGTCGAGAAGGTTTAGCGATTGAAGCAGGAT
TCTCATTACCGACAATCAGGGTTATTCGTACGTTGAATCAGTTGCTGGAGTGGCGAGAAAAGCCGTTAGTTATTCGGTGTGACAATGGCCCGGAGTTTAT
CAGTCATGAATTTGTACGCTGGGCAACTGAACAAGGTATTCGTATTGAATATATTCAACCAGGTAAGCCACAGCAGAATGCGTATATTGAACGTTATAAT
CGGACTATACGCTATAGCTGGCTAAGCAAGCATCTATTTGACACACTGGAAGAAGTGCAAGATTATGCAACGAGTTGGCTGTGGCATTACAACCATGAAA
GACCACACCAAGCAAACAAAGGAAAGCCACCTCTAATGGCTGCTTAACCTCTACTTTTAACTTCGGTTATTTATGGGAGGATTACC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 267 bp | 88 aa | 72 | 338 | + | No |
Description : First part of the transposase
ORF sequence :
MKTSKFTDSQIMSILKQAESGTPVAALCREHGMSNATFYKWRAKYGGMDTSLMARLKELEAENTRLKKMYAEERLKAEIIQEAMAKKW
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 876 bp | 291 aa | 270 | 1145 | + | No |
Description : Second part of the transposase
ORF sequence :
KDVCGRTIKGRDHPGSDGKKVVRPSCRRKMAQQAVAHYAISIRLACSAFSISETCYRYQAKLSDDNALIAEQLIELTEENTDWGFGLCFSYLRHVENYVW
NHKRVYRIYCELALNLRIKPRRRLKRHAPEPLKEPIRANQVWSLDFMHDQLIDGRKYRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLV
IRCDNGPEFISHEFVRWATEQGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATSWLWHYNHERPHQANKGKPPLMAA
NHKRVYRIYCELALNLRIKPRRRLKRHAPEPLKEPIRANQVWSLDFMHDQLIDGRKYRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLV
IRCDNGPEFISHEFVRWATEQGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATSWLWHYNHERPHQANKGKPPLMAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1074 bp | 358 aa | 72 | 1145 | + | Yes |
Chemistry : DDE
ORF sequence :
MKTSKFTDSQIMSILKQAESGTPVAALCREHGMSNATFYKWRAKYGGMDTSLMARLKELEAENTRLKKMYAEERLKAEIIQEAMAKKVVRPSCRRKMAQQ
AVAHYAISIRLACSAFSISETCYRYQAKLSDDNALIAEQLIELTEENTDWGFGLCFSYLRHVENYVWNHKRVYRIYCELALNLRIKPRRRLKRHAPEPLK
EPIRANQVWSLDFMHDQLIDGRKYRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEQGIRIEYIQPGKP
QQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATSWLWHYNHERPHQANKGKPPLMAA
AVAHYAISIRLACSAFSISETCYRYQAKLSDDNALIAEQLIELTEENTDWGFGLCFSYLRHVENYVWNHKRVYRIYCELALNLRIKPRRRLKRHAPEPLK
EPIRANQVWSLDFMHDQLIDGRKYRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEQGIRIEYIQPGKP
QQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATSWLWHYNHERPHQANKGKPPLMAA
Blast result :
Comments
ORFa is 71% aa similar with ISR1, IS1403, IS1404 and IS1122, 78% with ISD1. ORFb is 64% aa similar with ISD1, 59% with IS1400 and 54% with IS1403 and IS1404.
Note that the ends are ambiguous.The extremities was redefined -6 bp on the left end and -2 bp on the right end.
Note that the ends are ambiguous.The extremities was redefined -6 bp on the left end and -2 bp on the right end.
References
1] Koo,J.H., Jung,S.B., Byun,H.S. and Kim,Y.S. (1997) Biochim. Biophys. Acta 1354 (1), 49-54
2] Koo,J.H. and Kim,Y.S. (1999) Eur. J. Biochem. 266, 683-690.
2] Koo,J.H. and Kim,Y.S. (1999) Eur. J. Biochem. 266, 683-690.