ISAca1

  • Family IS3
  • Group IS407
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF121266 ND Acinetobacter calcoaceticus
Acinetobacter calcoaceticus
DNA section
IS Length : 1186 bp

Ends


IR Length : 18/21

IRL : GGTAATCCCCCCGAAAAATAAAAGGATGTAAAAGTAGAATTTTCTCTTAA
IRR : GGTAATCCTCCCATAAATAACCGAAGTTAAAAGTAGAGGTTAAGCAGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
TGCGAGAAGCACCATAGCGTTCGC4

DNA sequence

GGTAATCCCCCCGAAAAATAAAAGGATGTAAAAGTAGAATTTTCTCTTAAGATACAAGCAGGAGATTCTGCATGAAAACATCGAAATTTACTGACAGTCA
GATCATGTCCATCTTGAAGCAGGCGGAATCTGGCACACCTGTAGCCGCCCTTTGTCGTGAACACGGCATGAGTAATGCCACGTTTTATAAATGGCGTGCC
AAATATGGCGGTATGGATACCTCATTGATGGCTCGACTCAAAGAACTGGAAGCAGAAAATACCAGACTTAAAAAGATGTATGCGGAAGAACGATTAAAGG
CAGAGATCATCCAGGAAGCGATGGCAAAAAAGTGGTGAGGCCATCTTGCCGACGTAAGATGGCACAACAGGCAGTTGCCCACTATGCCATTAGCATTCGT
TTGGCTTGCAGCGCATTTAGTATCAGTGAAACCTGCTACCGTTATCAGGCTAAACTCAGTGATGACAATGCACTCATTGCAGAACAGCTCATTGAACTCA
CTGAAGAAAATACAGATTGGGGCTTTGGTCTGTGTTTCTCTTATCTACGTCATGTTGAAAATTATGTTTGGAATCACAAGCGTGTTTATCGTATTTACTG
TGAATTAGCACTGAATCTGCGTATTAAGCCAAGAAGAAGATTGAAACGACATGCACCAGAACCACTGAAAGAGCCGATCCGGGCAAATCAGGTCTGGTCA
CTGGATTTTATGCATGACCAGCTGATCGATGGTCGTAAGTATCGGCTATTGAATGTAATTGATGATTACCGTCGAGAAGGTTTAGCGATTGAAGCAGGAT
TCTCATTACCGACAATCAGGGTTATTCGTACGTTGAATCAGTTGCTGGAGTGGCGAGAAAAGCCGTTAGTTATTCGGTGTGACAATGGCCCGGAGTTTAT
CAGTCATGAATTTGTACGCTGGGCAACTGAACAAGGTATTCGTATTGAATATATTCAACCAGGTAAGCCACAGCAGAATGCGTATATTGAACGTTATAAT
CGGACTATACGCTATAGCTGGCTAAGCAAGCATCTATTTGACACACTGGAAGAAGTGCAAGATTATGCAACGAGTTGGCTGTGGCATTACAACCATGAAA
GACCACACCAAGCAAACAAAGGAAAGCCACCTCTAATGGCTGCTTAACCTCTACTTTTAACTTCGGTTATTTATGGGAGGATTACC
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
267 bp88 aa72338+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MKTSKFTDSQIMSILKQAESGTPVAALCREHGMSNATFYKWRAKYGGMDTSLMARLKELEAENTRLKKMYAEERLKAEIIQEAMAKKW

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
876 bp291 aa2701145+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KDVCGRTIKGRDHPGSDGKKVVRPSCRRKMAQQAVAHYAISIRLACSAFSISETCYRYQAKLSDDNALIAEQLIELTEENTDWGFGLCFSYLRHVENYVW
NHKRVYRIYCELALNLRIKPRRRLKRHAPEPLKEPIRANQVWSLDFMHDQLIDGRKYRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLV
IRCDNGPEFISHEFVRWATEQGIRIEYIQPGKPQQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATSWLWHYNHERPHQANKGKPPLMAA

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1074 bp358 aa721145+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKTSKFTDSQIMSILKQAESGTPVAALCREHGMSNATFYKWRAKYGGMDTSLMARLKELEAENTRLKKMYAEERLKAEIIQEAMAKKVVRPSCRRKMAQQ
AVAHYAISIRLACSAFSISETCYRYQAKLSDDNALIAEQLIELTEENTDWGFGLCFSYLRHVENYVWNHKRVYRIYCELALNLRIKPRRRLKRHAPEPLK
EPIRANQVWSLDFMHDQLIDGRKYRLLNVIDDYRREGLAIEAGFSLPTIRVIRTLNQLLEWREKPLVIRCDNGPEFISHEFVRWATEQGIRIEYIQPGKP
QQNAYIERYNRTIRYSWLSKHLFDTLEEVQDYATSWLWHYNHERPHQANKGKPPLMAA

 

Blast result :
Comments
ORFa is 71% aa similar with ISR1, IS1403, IS1404 and IS1122, 78% with ISD1. ORFb is 64% aa similar with ISD1, 59% with IS1400 and 54% with IS1403 and IS1404.
Note that the ends are ambiguous.The extremities was redefined -6 bp on the left end and -2 bp on the right end.
References
1] Koo,J.H., Jung,S.B., Byun,H.S. and Kim,Y.S. (1997) Biochim. Biophys. Acta 1354 (1), 49-54
2] Koo,J.H. and Kim,Y.S. (1999) Eur. J. Biochem. 266, 683-690.