ISElsp1
- Family IS3
- Group IS3
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| LNOK01000025.1 | ND | Elizabethkingia sp. | Elizabethkingia sp. Elizabethkingia genomosp. 4 G4122 |
DNA section
IS Length : 1348 bp
Ends
IR Length : 24/26
IRL : TGTCTCGTCCTGAAAAAGGTTGACTAAAAAACATCAGAAATAGTTATCCT
IRR : TGACTAGTCCTGAAAAAGGTTGACTAGTTTAATAAATCAAATATATTAAA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| NNNN | CTTGCCTCAGTCGCTG | 0 |
DNA sequence
TGTCTCGTCCTGAAAAAGGTTGACTAAAAAACATCAGAAATAGTTATCCTTATGAAATTAAAAAAAAGCCCATCCCCTGTACAAGAATACAGCGAGGGAT
TCAAAAGACAAGTTGTCTCCGAATATGAACGGGGATTATTTACTAAATCACAACTACAGACTCGATACAACATTCGAGGTAATTCCTGTATTTCCAGTTG
GTTAGTAAAATATGGTAACTTTACTTATTCAAAGCAACTAAGTAAAGGCAGGCCTATGAAAGATCCACAAAAGCAAAAGATTAAAGAGCTTGAAGCAGCT
TTAGCCAAAAAAGAGGAAGAACTTAAAGTGTTTCGGAAGTTCATAGAAATAGCTGAGCGTGAGCTAAAGATTGAAATTGTAAAAAAGTCTGGTTCCAATC
AATCCCGGAAATAAGGCCATATACCACTCTTTCAACTGAAGATATTTGCCGATTGTTTGGATACAGTAAACAAGCATATTACAAAAGAACAAAACAGTTT
AATTCTGGTAATAATCAGGAAAGAATTATAGAATTGGTCTTATCAATTCGTAAAAAGATGCCTAGGTTAGGCACCCGAAAACTTTATGCCATACTTAAGA
GTGATTTTAATAAGGAGAATATTCATATAGGAAGAGATCAGTTATTTAAGATTTTAAGATCAAATTATCTTTTAATTCCCCGAAGACATAGTTACTTTAA
AACAACGAATTCCAGACACTGGATGAAGAAATATCCTAATATCATCAAAGAAAAAGAGCCAGAATTTTCAGAGAAGATATGGGTAGCAGATATTACCTAT
CTGAAAACTAAAGAGAGAAATTATTATCTTCATTTAATTACTGATGCTTATTCGAAGAAAATTGTAGGATACGAATTGAGTGACAATTTACAAACGAGCT
CTACATTGAAAGCGTTAAAGCAAGCTATAAAGAGTAGAATCTATAAACATCCTTTAATTCACCACTCAGACAGAGGCTTACAATATTGTAGTAAAGAATA
TATCGAGGTGCTAAAGAAAAATGATATTCTTATCAGTATGACCGAGAACTCTGATCCTTATGAAAATGCCGTAGCAGAAAGAGTAAATGGTATTCTGAAA
TATGAATTTGGATTGATTGATACTTTTGAAAATTTTAAAAATCTTTCACAGCAAATTGATCAGTCAATTTACTATTATAATAATTTAAGACCGCACTTTT
CCTTGAATTACAACATTCCAAGTCAGGTACACATCAATAATAATGTGAAATTGAAAACTTATAAAAAACAAAATCAGAATAGAAAGATTCTACTCTGATT
TAATATATTTGATTTATTAAACTAGTCAACCTTTTTCAGGACTAGTCA
TCAAAAGACAAGTTGTCTCCGAATATGAACGGGGATTATTTACTAAATCACAACTACAGACTCGATACAACATTCGAGGTAATTCCTGTATTTCCAGTTG
GTTAGTAAAATATGGTAACTTTACTTATTCAAAGCAACTAAGTAAAGGCAGGCCTATGAAAGATCCACAAAAGCAAAAGATTAAAGAGCTTGAAGCAGCT
TTAGCCAAAAAAGAGGAAGAACTTAAAGTGTTTCGGAAGTTCATAGAAATAGCTGAGCGTGAGCTAAAGATTGAAATTGTAAAAAAGTCTGGTTCCAATC
AATCCCGGAAATAAGGCCATATACCACTCTTTCAACTGAAGATATTTGCCGATTGTTTGGATACAGTAAACAAGCATATTACAAAAGAACAAAACAGTTT
AATTCTGGTAATAATCAGGAAAGAATTATAGAATTGGTCTTATCAATTCGTAAAAAGATGCCTAGGTTAGGCACCCGAAAACTTTATGCCATACTTAAGA
GTGATTTTAATAAGGAGAATATTCATATAGGAAGAGATCAGTTATTTAAGATTTTAAGATCAAATTATCTTTTAATTCCCCGAAGACATAGTTACTTTAA
AACAACGAATTCCAGACACTGGATGAAGAAATATCCTAATATCATCAAAGAAAAAGAGCCAGAATTTTCAGAGAAGATATGGGTAGCAGATATTACCTAT
CTGAAAACTAAAGAGAGAAATTATTATCTTCATTTAATTACTGATGCTTATTCGAAGAAAATTGTAGGATACGAATTGAGTGACAATTTACAAACGAGCT
CTACATTGAAAGCGTTAAAGCAAGCTATAAAGAGTAGAATCTATAAACATCCTTTAATTCACCACTCAGACAGAGGCTTACAATATTGTAGTAAAGAATA
TATCGAGGTGCTAAAGAAAAATGATATTCTTATCAGTATGACCGAGAACTCTGATCCTTATGAAAATGCCGTAGCAGAAAGAGTAAATGGTATTCTGAAA
TATGAATTTGGATTGATTGATACTTTTGAAAATTTTAAAAATCTTTCACAGCAAATTGATCAGTCAATTTACTATTATAATAATTTAAGACCGCACTTTT
CCTTGAATTACAACATTCCAAGTCAGGTACACATCAATAATAATGTGAAATTGAAAACTTATAAAAAACAAAATCAGAATAGAAAGATTCTACTCTGATT
TAATATATTTGATTTATTAAACTAGTCAACCTTTTTCAGGACTAGTCA
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence : No
- Secondary structure : stem-loop
Recoding motif :
GAGCTAAAGATTGAAATTGTAAAAAAGTCTGGTTCCAATCAATCCCGGAA
..................................((((.(((((..((((
ATAAGGCCATATACCACTCTTTCAACTGAAGATATTTGCCGATTGTTTGG
(((.((.......))..(((((.....)))))))))).))))))).))))
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 363 bp | 120 aa | 52 | 414 | + | No |
Description : First part of the transposase
ORF sequence :
MKLKKSPSPVQEYSEGFKRQVVSEYERGLFTKSQLQTRYNIRGNSCISSWLVKYGNFTYSKQLSKGRPMKDPQKQKIKELEAALAKKEEELKVFRKFIEI
AERELKIEIVKKSGSNQSRK
AERELKIEIVKKSGSNQSRK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 924 bp | 307 aa | 375 | 1298 | + | No |
Description : Second part of the transposase
ORF sequence :
NCKKVWFQSIPEIRPYTTLSTEDICRLFGYSKQAYYKRTKQFNSGNNQERIIELVLSIRKKMPRLGTRKLYAILKSDFNKENIHIGRDQLFKILRSNYLL
IPRRHSYFKTTNSRHWMKKYPNIIKEKEPEFSEKIWVADITYLKTKERNYYLHLITDAYSKKIVGYELSDNLQTSSTLKALKQAIKSRIYKHPLIHHSDR
GLQYCSKEYIEVLKKNDILISMTENSDPYENAVAERVNGILKYEFGLIDTFENFKNLSQQIDQSIYYYNNLRPHFSLNYNIPSQVHINNNVKLKTYKKQN
QNRKILL
IPRRHSYFKTTNSRHWMKKYPNIIKEKEPEFSEKIWVADITYLKTKERNYYLHLITDAYSKKIVGYELSDNLQTSSTLKALKQAIKSRIYKHPLIHHSDR
GLQYCSKEYIEVLKKNDILISMTENSDPYENAVAERVNGILKYEFGLIDTFENFKNLSQQIDQSIYYYNNLRPHFSLNYNIPSQVHINNNVKLKTYKKQN
QNRKILL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1247 bp | 415 aa | 52 | 1298 | + | Yes |
Chemistry : DDE
ORF sequence :
MKLKKSPSPVQEYSEGFKRQVVSEYERGLFTKSQLQTRYNIRGNSCISSWLVKYGNFTYSKQLSKGRPMKDPQKQKIKELEAALAKKEEELKVFRKFIEI
AERELKIEIVKKVWFQSIPEIRPYTTLSTEDICRLFGYSKQAYYKRTKQFNSGNNQERIIELVLSIRKKMPRLGTRKLYAILKSDFNKENIHIGRDQLFK
ILRSNYLLIPRRHSYFKTTNSRHWMKKYPNIIKEKEPEFSEKIWVADITYLKTKERNYYLHLITDAYSKKIVGYELSDNLQTSSTLKALKQAIKSRIYKH
PLIHHSDRGLQYCSKEYIEVLKKNDILISMTENSDPYENAVAERVNGILKYEFGLIDTFENFKNLSQQIDQSIYYYNNLRPHFSLNYNIPSQVHINNNVK
LKTYKKQNQNRKILL
AERELKIEIVKKVWFQSIPEIRPYTTLSTEDICRLFGYSKQAYYKRTKQFNSGNNQERIIELVLSIRKKMPRLGTRKLYAILKSDFNKENIHIGRDQLFK
ILRSNYLLIPRRHSYFKTTNSRHWMKKYPNIIKEKEPEFSEKIWVADITYLKTKERNYYLHLITDAYSKKIVGYELSDNLQTSSTLKALKQAIKSRIYKH
PLIHHSDRGLQYCSKEYIEVLKKNDILISMTENSDPYENAVAERVNGILKYEFGLIDTFENFKNLSQQIDQSIYYYNNLRPHFSLNYNIPSQVHINNNVK
LKTYKKQNQNRKILL
Blast result :
Comments
ISElsp1 is 61% (ORFAB transposase) aa similar to ISSpe1. The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Ainsley Nicholson (2015) Direct submission.