ISAav1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AF086815 ND Acidovorax avenae
Acidovorax avenae subsp. citrulli
DNA section
IS Length : 2532 bp

Ends


IR Length : 27

IRL : TGTTGAATCACGAGCAAAACTGAGCCACTAAAGGGCATCCGTCACGTCCA
IRR : TGTTGAATCACGAGCAAAACTGAGCCAGGCGTCACGTCCAAAACTGAGCC

Insertion site


Left flankDirect repeatRight flankDR Length
GATAGGCGACGCTGCTGTACCA2

DNA sequence

TGTTGAATCACGAGCAAAACTGAGCCACTAAAGGGCATCCGTCACGTCCAATTTTGAGCCACGTTATCCACCTACCCTGCTGTTTTTTGCAGCAGTGGGG
TGCAGGAGTGATCAACGTGAGCACATTGAGCAAATTGCGCCGCATGGTCCACCGTGACCACCTGAGCGTGCGCGAAGCCAGCCGAAGGCTGGGCATCTCC
CGCAATACAGCCGCCAAGTGGCTCGAGGCCGACGAGATGGTCGAGCCCCGCTACCCCAAGCGGGCCTCTCTTCCCAGCGTTCTCGATCCCTACAAGGAAC
AGCTGGCCACCTGGCTGAAGGCCGACAGCCACCGCAACAAGCGCGAGCGCCGTGGCATCAAGGCCATGTTCCGGGCACTGCAGGCCATGGGCTACCCTGG
CAGCCGAGGCCCGGTCTATGAGTTCGCCAAGCGCTGGCAGCAGGCACAGTCCGACGCGCCAACCCGAATGGCCTTCGTACCCATGAGTTTCGAGATGGGC
GAGGCCTTCCAGTTCGACTGGAGCTGTGAGTACCTGTTCGTGGGCGGCATGCGCAAACGCCTGGAGGCTTCGCACACCAAGCTGGCGGCCAGTCGGGCCT
TCATGCTCACCGCCTACTTCAGCCAGGCCCACGAGATGTTGTTCGACGCTCATGCCCGAGCCTTTGCTGCTTTTGGTGGCGTGCCGCGCCGGGGCATCTA
CGACAACATGAAGACCGCCGTCGACAAGGTTGGTCAGGGCAAGAGCCGCACCGTCAATGCGCGCTTCGAGGCCATGACGGGTCACTACCTGTTCGAGCCA
GAGTTCTGCAACCGGGCCGCAGGCTGGGAGAAGGGTATCGTCGAGAAGAACGTGCAGGATCGCCGTCGTGGCATCTGGATGGAGGCAGCGGAACGCCGCT
GGCCCGACCTGGAGAGCCTCAATGCGTGGCTGCACCAGGCTTGCCTGGACGCCTGGCATGAGCTGCCTCACCCCGAGTGGCCAGAGCTGACGATCGCCGA
CGTCTGGCAGCAAGAGCAGACGCACCTGATGCCCAACCCTGCGCCATTTGATGGCTATGTTGAGCAGATGGTGCGGGTGACGGCCACCTCGCTGATCCAC
TACCAGCGCAATCGCTACAGCGTGCCATGCGAGTGGGCCCACACCAGTGTCAGCGTGCGGGCTTATCCCGACCGCCTGGTGGTGGTCGGCCCCAACTCGG
AACCGCCAGAACAGCCGGTGAGCCTGCCGCGCAGCTTCGAGCGTGGCCAGACCTTGTACGACTGGCGGCACTACGTCAGCTTGCTCGAACGCAAGCCCGG
CGCTTTGCGCAACGGCGCCCCCTTCAAGACCATGCCCGAGCCCTTGCAGCATCTGCAAGCGCACCTGCTTCGCCACCCTGGCGGCGACCGGGTGATGGCC
CAGGTGCTCATGGCCATCACCCTGCACGGGCTCGATGACGTGCTGGTGGCCGTGGAGTTGGCGCTGCAATCGGGCCGCGTGAGTGCCGACCATGTGCTCA
ACGTGCTGGCCAGGCTCAAGGAGCCCCAAGCCGTGCAAAGCCTGCCTGAAGCAGCCTTGCCTTCACTGACGCTGCACGAGCCGCCTCAGGCCGACGTGTC
GCGCTACGACAGCCTGCGCCAGTCCCAGGAGGATGACCATGTCCAATGACATCGCAGCAAGCCTCAAGGGCTTGAGCCTGCACGGCATGGCCAGTGCCTG
GCCGGAACTGCTGGGCATCGCCCGGCTCAAATCGCTCGACCACGAAGCCCTTCTGCATCAGCTCATCAAGGCCGAGGGCGCGCACCGGGAAGTGCGCTCC
ATGGCCTACCAGATGCGGGTGGCCCGATTCCCTCACCACCGCGATCTGGCTGGCTTTGCCTTTGATCAGGCCCAAGTGGACGAGGCGCTGGTGCGTCAGC
TGCACGAGTTCAAGTTCATCGACTCAGCCCACAACGTGGTCTTCGTGGGCGGCCCAGGCACGGGCAAAACACACCTTGCCACAAGCCTGGGCGTTCATGC
CATCCGTGCACATGGTAAGCGGGTGCGCTTCTTCTCGACCGTCGAGCTGGTCAATCTGCTGGAGGCCGAGAAAGCTCAAGGTAAGGCCGGGCAACTGGCA
CATCGGCTCATGTACGTGGATCTCGTCATCCTCGACGAGATGGGCTATTTGCCCTTCAGTCAAGCCGGTGGGGCCTTGCTGTTCCACCTGCTGTCCAAGC
TGTACGAGCGCACCAGCGTGGTCGTCACCACCAACCTGTCGTTCTCGGAGTGGGCCAGCGTGTTCGGCGACGCCAAGATGACCACCGCGCTACTCGACCG
ACTCACCCATCACTGCCACATCGTCGAAACCGGCAACCAGAGCTGGCGCTTCAGGCACTCGACTGCACAACCGTCTTCGATCATCAGAGCCACACGAACC
AAAACGGCCAAAGGAGCACCCCAGACAGATCACGCAGTAGACTTATCCACATCCGAACAGTCCATTTCTTCGTCAACTTAGTGGCTCAGTTTTGGACGTG
ACGCCTGGCTCAGTTTTGCTCGTGATTCAACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1533 bp510 aa1171649+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSTLSKLRRMVHRDHLSVREASRRLGISRNTAAKWLEADEMVEPRYPKRASLPSVLDPYKEQLATWLKADSHRNKRERRGIKAMFRALQAMGYPGSRGPV
YEFAKRWQQAQSDAPTRMAFVPMSFEMGEAFQFDWSCEYLFVGGMRKRLEASHTKLAASRAFMLTAYFSQAHEMLFDAHARAFAAFGGVPRRGIYDNMKT
AVDKVGQGKSRTVNARFEAMTGHYLFEPEFCNRAAGWEKGIVEKNVQDRRRGIWMEAAERRWPDLESLNAWLHQACLDAWHELPHPEWPELTIADVWQQE
QTHLMPNPAPFDGYVEQMVRVTATSLIHYQRNRYSVPCEWAHTSVSVRAYPDRLVVVGPNSEPPEQPVSLPRSFERGQTLYDWRHYVSLLERKPGALRNG
APFKTMPEPLQHLQAHLLRHPGGDRVMAQVLMAITLHGLDDVLVAVELALQSGRVSADHVLNVLARLKEPQAVQSLPEAALPSLTLHEPPQADVSRYDSL
RQSQEDDHVQ

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
843 bp280 aa16392481+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSNDIAASLKGLSLHGMASAWPELLGIARLKSLDHEALLHQLIKAEGAHREVRSMAYQMRVARFPHHRDLAGFAFDQAQVDEALVRQLHEFKFIDSAHNV
VFVGGPGTGKTHLATSLGVHAIRAHGKRVRFFSTVELVNLLEAEKAQGKAGQLAHRLMYVDLVILDEMGYLPFSQAGGALLFHLLSKLYERTSVVVTTNL
SFSEWASVFGDAKMTTALLDRLTHHCHIVETGNQSWRFRHSTAQPSSIIRATRTKTAKGAPQTDHAVDLSTSEQSISSST

 

Blast result :
Comments
There are two copies of ISAav1. ISAav1 is 66% (for ORF1) and 81% (for ORF2)aa similar to IS1600.
References
1] Eaton, R.W. (2001) Direct submission GenBank.