ISAlg2

  • Family IS1595
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
MDJD01000054 ND Algibacter sp.
Algibacter sp. SK-16
DNA section
IS Length : 988 bp

Ends


IR Length : 22/25

IRL : TTGAATATCCGTAATTACTAATAATTGATTAAAAATGCTTATTTTGTTAA
IRR : TTGAATATCCGTTTTTACTCATAATCCAGTAATTCCAGCTATTACTAGTC

Insertion site


Left flankDirect repeatRight flankDR Length
CAATTTATAA AAAGCAAA TT AAAGCAAA ATTGAAAAATACTT0
CGTGATATTA TAAAGCTTT TAAAGCTT TTTATTTAACCCCT0
GTTTTATTTA AATTATTT TT AATTATTT AAAACTTCACCAAA0
TAAAAAGTC AATAACTTT AAATAACTTT TAAGTAATATCAAA0
TTTATTGATAAATTCTTTTCTTGATTTAAATTA9
GTTATTTCAAATAAAAATATAATAAGAAAGAAA9

DNA sequence

TTGAATATCCGTAATTACTAATAATTGATTAAAAATGCTTATTTTGTTAAAAAAACAAGTCATGAATATTTTTAGTTTTACCGCCCATTTTGATAGTGAA
GAAGCTTGTCGTACTCATTTTAAGCAAGAAAGAGATAAGATAGGAGTTAAATGCCAGCGTTGTGGTCATACTCATCACTATTGGATAAAGGCTGTTTGGA
GCTATGAGTGCAAATCTTGTAGAGCCCGTACATCTTTGAGAAGTGGTACTATTATGCAAAATTCTAATCTATCTTTTATGATATGGTATAAGACAATGTT
TTTGATGACTACTACCAAGAAAGGATTTTCTTCTAAAGAAATCCAGAAACAGCTTGGTTTAAAAAGGTATGAACCTGTCTGGGCGATGGTTCATAAACTT
CGTAAAGCAATGGGTAACAGAGATGCCCGCTATACTTTAGAAGGTATGATTGAAATGGATGAAGGTTATTTTACGGTCGAATCTTCTGAAATAGAGCAAC
AAAAAGGAAAAAGAGGTCGTGGTGCAGTAGGAAAGCAAAACGTAATGATAATGGCAGAATCCACTCCCTTAGAAGACCCGAAAACCAACAAGAAGGATCG
CCAATGTCGCTACTTTAAAGCCAAAGTGCTGACAGATCATAAGGCTGATGGTGTAAATGATACTGTTAAAGAGTCTTTAGACGAACAAAGCATTGTTTTT
ACTGATAAAAGTACTTCATATGTTGATATTGCTGATTTTGTAGAACTACATATTAGTGAAAAATCAGATAAACAAACTACTAAAGAGACCTTGAAATGGG
TACATATTACCATAAGCAATGCCAAACGAAACTTACTTGGAAATTATCACAAAATTAAAGGCAAATACCTACAACTCTACCTAAACGAATTTGTTTACAA
ACTCAATCGAAGATATTTTGAGGAAAAACTATTCGATAGACTAGTAATAGCTGGAATTACTGGATTATGAGTAAAAACGGATATTCAA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
909 bp302 aa62970+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNIFSFTAHFDSEEACRTHFKQERDKIGVKCQRCGHTHHYWIKAVWSYECKSCRARTSLRSGTIMQNSNLSFMIWYKTMFLMTTTKKGFSSKEIQKQLGL
KRYEPVWAMVHKLRKAMGNRDARYTLEGMIEMDEGYFTVESSEIEQQKGKRGRGAVGKQNVMIMAESTPLEDPKTNKKDRQCRYFKAKVLTDHKADGVND
TVKESLDEQSIVFTDKSTSYVDIADFVELHISEKSDKQTTKETLKWVHITISNAKRNLLGNYHKIKGKYLQLYLNEFVYKLNRRYFEEKLFDRLVIAGIT
GL

 

Blast result :
Comments
ISAlg2 is 92% aa similar to ISFba1.
References
1] ISfinder annotation (2017)
2] Wong,S.-K., Yoshizawa,S., Nakajima,Y., Ogura,Y., Tetsuya,H. and Hamasaki,K. (2016) Direct GenBank submission.