ISAac1

  • Family IS200/IS605
  • Group IS200
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
D64078 ND Actinobacillus actinomycetemcomitans
Actinobacillus actinomycetemcomitans Y4
DNA section
IS Length : 712 bp

Ends


Left end : TATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAA II struct. : Yes
Right end : TCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGTGCGGATTGTTAT II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TAACATTTATTCAGCCTTTTTATGATTTTTTTGTACttat

DNA sequence

TATTATAGCTAGACAACGTCTAGCTATAAACAAAACCGTACGCTATGCGTATGGAATCAAAGAGCTTAAGCGGTCAACATAAAAAATCCTCACTATATAA
GAATAAGGCTGACAACCCAAATTCAAGATATAGGAGGATAAGTCAATGGTAAGTAAAGCCAATGACGATTCAAGTCTATCACATACCAAATGGAACTGTA
AGTATCACATCGTCTTCACCCCGAAATATAGAAGAAAAGCCATTTACGGAAGGCTTAGAACGGATATAGGTAGCATATTAAGGCAGTTATGCGACTATAA
AAATGTGGAAATAATAGAAGCGCACGCAATGAAAGAGCATATTCATATGCTTCTAAAAATTCCGCCGAAATTATCGGTATCAAGTTTTATGGGGTATTTA
AAGGGAAAATCATTTGAAAGGCACGCAAACCTAAAATACAACTATGGTAACCGACATTTTTGGTCGAAAGGCTATTATGTAAGCACGGTAGGGCTAAATA
CAAAGGTAGTGTAGGAGTATATAAGGAATCAGGAAAAGGAAGACATGATTCAGGATAATTTATCGAAGAAAGAATATGTGGACCCCTTTAAGGGGTAGGT
CATAGAGTTATTTCACGGTTGTTGGTTTTCATATGCCCTTTGAAGGGCATGTGAAGTACTAGGTCTTTATAGAGTAGCCTAAAAACTGCACGTTTTACGT
GCGGATTGTTAT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
453 bp150 aa146598+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MVSKANDDSSLSHTKWNCKYHIVFTPKYRRKAIYGRLRTDIGSILRQLCDYKNVEIIEAHAMKEHIHMLLKIPPKLSVSSFMGYLKGKSFERHANLKYNY
GNRHFWSKGYYVSTVGLNTKVV*EYIRNQEKEDMIQDNLSKKEYVDPFKG

 

Blast result :
Comments
78% aa homologous with IS200.
There is a STOP codon in frame, this transposase is probably inactive.
This file was updated April 3 2012.
References
1] Hayashida,H., Hotokezaka,H., Ohara,N., Kimura,M., Takagi,O. and Yamada,T.(1996) Microbiology 142, 2449-2452